CAJ | 학술논문

目的研究运动训练对血管性痴呆(VD)大鼠学习记忆功能恢复及组织生长相关蛋白-43(GAP-43)表达的影响.方法选择SD雌性大鼠44只,随机分为运动组20只、制动组20只和假手术组4只,采用双侧颈总动脉反复缺血再灌注加降血压法制作血管性痴呆大鼠模型.运动组大鼠每天进行滚筒、转棒训练,时间为1 h;制动组大鼠被限制自由活动;假手术组置于普通笼中自由活动.3组分别于术后第27,28天进行跳台试验测定学习、记忆能力.取脑组织采用免疫组织化学染色方法观察不同时间点海马CA1区GAP-43的表达.结果以跳台试验对学习记忆能力进行的评估示运动组优于制动组(P<0.01),运动组GAP-43在海马CA1区的表达第1天后逐渐增高,第7天时达高峰,较制动组和假手术组均有明显增加(P<0.01).结论运动训练可改善VD大鼠学习记忆能力,其机制可能与运动训练能促进海马CA1区上GAP-43表达有关.
목적 연구운동훈련대혈관성치태(VD)대서학습기억공능회복급조직생장상관단백-43(GAP-43)표체적영향.방법 선택SD자성대서44지,수궤분위운동조20지、제동조20지화가수술조4지,채용쌍측경총동맥반복결혈재관주가강혈압법제작혈관성치태대서모형.운동조대서매천진행곤통、전봉훈련,시간위1 h;제동조대서피한제자유활동;가수술조치우보통롱중자유활동.3조분별우술후제27,28천진행도태시험측정학습、기억능력.취뇌조직채용면역조직화학염색방법관찰불동시간점해마CA1구GAP-43적표체.결과 이도태시험대학습기억능력진행적평고시운동조우우제동조(P<0.01),운동조GAP-43재해마CA1구적표체제1천후축점증고,제7천시체고봉,교제동조화가수술조균유명현증가(P<0.01).결론 운동훈련가개선VD대서학습기억능력,기궤제가능여운동훈련능촉진해마CA1구상GAP-43표체유관.
Objective To study the effects of rehabilitation training on learning and memory ability and the expression of growth-associated protein-43(GAP-43)in rats with vascular dementia.Methods Forty-four female Sprague-Dawley rats were randomly assigned to a rehabilitation group(n=20),an immobilization group(n=20),and a sham-operation group(n=4).The rats in the former 2 groups were operated on to establish the experimental vascular dementia model by repeatedly ischemia/reperfusion injury of brain induced by ligation of bilateral common carotid arteries and lowering of blood pressure induced by intraabdominal injection of sodium nitroprusside.The rats in rehabilitation group were administered with rotating bar and rolling cage exercises for 1 hour once daily,while those in the immobilization group were immobilized and without any exercise:the rats in sham-operation group could move freely in cage.Learning and memory tests were preformed by using step-down avoidance test at the 27th and 28th days after operation.Immunohistochemical staining was used to detect GAP-43 expression in hippocampus CA1 area at different time points after operation. Results The rats in rehabilitation group demonstrated better learning and memory ability than those in immobilization group(P<0.01),and more GAP-43 expression in hippocampus CA1 ar-ea than those in immobilization group and sham-operation(P<0.01).Conclusion Rehabilitation training can im-prove the learning and memory ability of rats with experimental vascular dementia,and the mechanism is probably re-lated to the increase of GAP-43 in hippocampus CA1 area.