中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2010年
2期
126-129
,共4页
王悦%宋诗铎%祁伟%刘德梦%王玉宝%王哲%郭文学
王悅%宋詩鐸%祁偉%劉德夢%王玉寶%王哲%郭文學
왕열%송시탁%기위%류덕몽%왕옥보%왕철%곽문학
鲍曼不动杆菌%外排泵%多重耐药性%adeRS
鮑曼不動桿菌%外排泵%多重耐藥性%adeRS
포만불동간균%외배빙%다중내약성%adeRS
Acinetobacter baumannii%Efflux pump%Multidrug resistance%adeRS
目的 研究鲍曼不动杆菌(Acinetobacter baumannii)临床株外排泵AdeABC的表达与耐药的关系及表达调控.方法 微量肉汤稀释法检测鲍曼不动杆菌临床株对抗菌药物的敏感性及泵抑制剂作用,RT-PCR检测泵基因adeB的mRNA表达水平,PCR扩增泵调控基因adeRS并测序分析.结果 30株多重耐药的鲍曼不动杆菌临床株及5株敏感株均存在泵结构基因片段adeB和调控基因adeRS,随机选取15株多重耐药株中均检测到adeB的mRNA表达,而在5株敏感株中无表达.测定2株多重耐药株调控基因adeRS序列均出现基因突变,发生氨基酸替代及缺失.结论 鲍曼不动杆菌临床株外排泵AdeABC的表达可能与耐药性有关,在多重耐药株中存在着调控基因adeRS的基因序列变化.
目的 研究鮑曼不動桿菌(Acinetobacter baumannii)臨床株外排泵AdeABC的錶達與耐藥的關繫及錶達調控.方法 微量肉湯稀釋法檢測鮑曼不動桿菌臨床株對抗菌藥物的敏感性及泵抑製劑作用,RT-PCR檢測泵基因adeB的mRNA錶達水平,PCR擴增泵調控基因adeRS併測序分析.結果 30株多重耐藥的鮑曼不動桿菌臨床株及5株敏感株均存在泵結構基因片段adeB和調控基因adeRS,隨機選取15株多重耐藥株中均檢測到adeB的mRNA錶達,而在5株敏感株中無錶達.測定2株多重耐藥株調控基因adeRS序列均齣現基因突變,髮生氨基痠替代及缺失.結論 鮑曼不動桿菌臨床株外排泵AdeABC的錶達可能與耐藥性有關,在多重耐藥株中存在著調控基因adeRS的基因序列變化.
목적 연구포만불동간균(Acinetobacter baumannii)림상주외배빙AdeABC적표체여내약적관계급표체조공.방법 미량육탕희석법검측포만불동간균림상주대항균약물적민감성급빙억제제작용,RT-PCR검측빙기인adeB적mRNA표체수평,PCR확증빙조공기인adeRS병측서분석.결과 30주다중내약적포만불동간균림상주급5주민감주균존재빙결구기인편단adeB화조공기인adeRS,수궤선취15주다중내약주중균검측도adeB적mRNA표체,이재5주민감주중무표체.측정2주다중내약주조공기인adeRS서렬균출현기인돌변,발생안기산체대급결실.결론 포만불동간균림상주외배빙AdeABC적표체가능여내약성유관,재다중내약주중존재착조공기인adeRS적기인서렬변화.
Objective To study the expression of active efflux pump AdeABC in clinical Acineto-bacter baumannii islates and whether this efflux pump confers resistance to antibiotics. Methods The anti-biotic susceptibility and the function of efflux pump inhibitor were tested by micro-dilution broth method. The expression of adeB was examined by RT-PCR. The controlling gene adeRS was amplified by PCR and se-quenced. Results Thirty multidrug resistance Acinetobacter baumannii isolates and 5 sensitive isolates for PCR were both abtained the expected products of adeB and adeRS. The mRNA expression of adeB in 15 multidrug resistance(MDR) isolates were positive, but there was no expression of adeB in 5 sensitive iso-lates. The mutations of adeRS existed in 2 MDR isolates. Conclusion The expression of AdeABC may in-volved in the resistant mechanisms of the clinical MDR Acinetobacter baumannii isolates.
