CAJ | 학술논문

目的探讨白介素10(IL-10)对大鼠脑缺血再灌注后E-选择素及L-选择素表达的抑制作用.方法 72只成年雄性SD大鼠随机均分为假手术组,脑缺st/再灌注组,溶剂对照组和IL-10干预组.IL-10干预组经侧脑室注射IL-10(1μg溶解于5μl 5 mol/L NaP),对照组经侧脑室注射溶剂(5μl 5 mol/L NaP).免疫组织化学法、RT-PCR法和Western印迹法检测E-选择素及L-选择素的表达变化.结果脑缺血再灌注组E-选择素及L-选择素表达明显高于假手术组(P<0.05).IL-10干预组E-选择素及L-选择素阳性血管(18.8±1.9,15.8±2.4)明显少于溶剂对照组(24.7±2.4,20.9±3.3)(P<0.05).IL-10干预组E-选择素及L-选择素基因表达(0.431±0.029,0.318±0.048)明显低于溶剂对照组(0.497±0.019,0.433±0.087)(P<0.05).IL-10干预组E-选择素及L-选择素蛋白表达(0.349±0.037,0.296±0.035)明显低于溶剂对照组(0.421±0.043,0.348±0.044)(P<0.05).结论 IL-10可抑制大鼠脑缺血再灌注后E-选择素及L-选择素的表达.
목적 탐토백개소10(IL-10)대대서뇌결혈재관주후E-선택소급L-선택소표체적억제작용.방법 72지성년웅성SD대서수궤균분위가수술조,뇌결st/재관주조,용제대조조화IL-10간예조.IL-10간예조경측뇌실주사IL-10(1μg용해우5μl 5 mol/L NaP),대조조경측뇌실주사용제(5μl 5 mol/L NaP).면역조직화학법、RT-PCR법화Western인적법검측E-선택소급L-선택소적표체변화.결과 뇌결혈재관주조E-선택소급L-선택소표체명현고우가수술조(P<0.05).IL-10간예조E-선택소급L-선택소양성혈관(18.8±1.9,15.8±2.4)명현소우용제대조조(24.7±2.4,20.9±3.3)(P<0.05).IL-10간예조E-선택소급L-선택소기인표체(0.431±0.029,0.318±0.048)명현저우용제대조조(0.497±0.019,0.433±0.087)(P<0.05).IL-10간예조E-선택소급L-선택소단백표체(0.349±0.037,0.296±0.035)명현저우용제대조조(0.421±0.043,0.348±0.044)(P<0.05).결론 IL-10가억제대서뇌결혈재관주후E-선택소급L-선택소적표체.
Objective To investigate the inhibiting effects of interleukin-10 (IL-10) on the expression of E-selectin and L-selectin in cerebral ischemia-reperfusions. Methods Seventy-two adult male Sprague-Dawley rats were randomly divided into 4 equal groups, cerebral ischemia-reperfusion (I/R) group undergoing middle cerebral artery occlusion with Longa's thread method, IL-10 group undergoing lateral ventricle injection of IL-10 after the establishment of I/R model, Vehicle group undergoing lateral ventricle injection of nonnM aaline after the establishment of I/R model, and sham operation (Sham) group. Twenty-four hours later the rata were killed with their brains taken out. Immunohistochemistry, RT-PCR and Western blotting were used to detect the mRNA and protein expression of E-selectin and L-selectin. Results The E-selectin and L-selectin expression levels of the I/R group were significantly up-regulated compared with the Sham group (both P < 0.05). The numbers of E-selectin and L-selectin positive vessels of the IL-10 group were 18.8±1.9/10 HP fields and 15.8 + 2.4/10 HP fields respectively, both significantly less than those of the vehicle group (24.7±2.4/10 HP fields and 20.9 + 3.3/10 HP fields respectively, both P <0.05). The E-selectin and L-selectin gene mRNA expression levels of the IL-10 group were (0.431±0.029) and (0.318±0.048) respectively, both significantly lower than those of the Vehicle group [(0.497±0.019) and (0.433±0.087) respectively, both P < 0.05]. The E-selectin and L-selectin protein expression levels of the IL-10 group were (0.349±0.037) and (0.296±0.035) respectively, both significantly lower t6han those of the Vehicle group [(0.421±0.043,) and (0.348±0.044) respectively, both P < 0.05]. Conclusions IL-10 suppresses the expression of E-selectin and L-selectin in cerebral ischemia- reperfusion.