中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2010年
3期
221-226
,共6页
哌仑西平%受体,毒蕈碱%近视%豚鼠
哌崙西平%受體,毒蕈堿%近視%豚鼠
고륜서평%수체,독심감%근시%돈서
Pirenzepine%Receptors,muscarinic%Myopia%Guinea pigs
目的 探讨玻璃体腔注射选择性M_1受体拮抗剂哌仑西平对豚鼠形觉剥夺性近视眼视网膜、脉络膜、巩膜和虹膜-睫状体组织中M_1和M_4受体mRNA表达的影响.方法 采用随机分组设计的实验研究.1～2周龄的三色豚鼠24只,随机分为4组:正常对照组(N)6只,单纯形觉剥夺组(FDM)6只,药物对照组(S)6只,哌仑西平组(P)6只,均以左眼为实验眼,P组隔日玻璃体腔注射500μg哌仑西平;S组隔日玻璃体腔注射生理盐水.21 d后结束实验.提取各组眼视网膜、脉络膜、巩膜和虹膜-睫状体组织总RNA,半定量RT-PCR检测M_1和M_4亚型mRNA表达变化.3组间数据的比较采用单因素方差分析和Tukey post hoc检验.结果 21d时,FDM与N组相比较,FDM组眼轴相对延长0.29mm,产生相对近视-4.92 D,差异有统计学意义(P<0.001).P组与S组相比较,眼轴相对减少了0.30 mm,产生+0.88 D的相对远视,差异均有统计学意义(P<0.001).S组与FDM之间屈光度数的差异无统计学意义;而眼轴变化有统计学意义(S组相对延长0.08 mm,而FDM组相对延长0.29/mm).半定量PCR结果显示:P组与S组相比较,其视网膜、脉络膜和虹膜睫状体组织M_1和M_4亚型mRNA的表达差异无统计学意义(P>0.05).而在后极部巩膜组织,M_1和M_4亚型mRNA的表达较药物对照组显著性增加(P<0.05),其中M_1受体表达增加19.16%,M_4受体表达增加64.29%.结论 哌仑西平能够有效抑制豚鼠形觉剥夺近视的发展.巩膜组织M_1和M_4亚型及其胆碱能通路可能参与M受体拮抗剂抑制近视发展.
目的 探討玻璃體腔註射選擇性M_1受體拮抗劑哌崙西平對豚鼠形覺剝奪性近視眼視網膜、脈絡膜、鞏膜和虹膜-睫狀體組織中M_1和M_4受體mRNA錶達的影響.方法 採用隨機分組設計的實驗研究.1～2週齡的三色豚鼠24隻,隨機分為4組:正常對照組(N)6隻,單純形覺剝奪組(FDM)6隻,藥物對照組(S)6隻,哌崙西平組(P)6隻,均以左眼為實驗眼,P組隔日玻璃體腔註射500μg哌崙西平;S組隔日玻璃體腔註射生理鹽水.21 d後結束實驗.提取各組眼視網膜、脈絡膜、鞏膜和虹膜-睫狀體組織總RNA,半定量RT-PCR檢測M_1和M_4亞型mRNA錶達變化.3組間數據的比較採用單因素方差分析和Tukey post hoc檢驗.結果 21d時,FDM與N組相比較,FDM組眼軸相對延長0.29mm,產生相對近視-4.92 D,差異有統計學意義(P<0.001).P組與S組相比較,眼軸相對減少瞭0.30 mm,產生+0.88 D的相對遠視,差異均有統計學意義(P<0.001).S組與FDM之間屈光度數的差異無統計學意義;而眼軸變化有統計學意義(S組相對延長0.08 mm,而FDM組相對延長0.29/mm).半定量PCR結果顯示:P組與S組相比較,其視網膜、脈絡膜和虹膜睫狀體組織M_1和M_4亞型mRNA的錶達差異無統計學意義(P>0.05).而在後極部鞏膜組織,M_1和M_4亞型mRNA的錶達較藥物對照組顯著性增加(P<0.05),其中M_1受體錶達增加19.16%,M_4受體錶達增加64.29%.結論 哌崙西平能夠有效抑製豚鼠形覺剝奪近視的髮展.鞏膜組織M_1和M_4亞型及其膽堿能通路可能參與M受體拮抗劑抑製近視髮展.
목적 탐토파리체강주사선택성M_1수체길항제고륜서평대돈서형각박탈성근시안시망막、맥락막、공막화홍막-첩상체조직중M_1화M_4수체mRNA표체적영향.방법 채용수궤분조설계적실험연구.1～2주령적삼색돈서24지,수궤분위4조:정상대조조(N)6지,단순형각박탈조(FDM)6지,약물대조조(S)6지,고륜서평조(P)6지,균이좌안위실험안,P조격일파리체강주사500μg고륜서평;S조격일파리체강주사생리염수.21 d후결속실험.제취각조안시망막、맥락막、공막화홍막-첩상체조직총RNA,반정량RT-PCR검측M_1화M_4아형mRNA표체변화.3조간수거적비교채용단인소방차분석화Tukey post hoc검험.결과 21d시,FDM여N조상비교,FDM조안축상대연장0.29mm,산생상대근시-4.92 D,차이유통계학의의(P<0.001).P조여S조상비교,안축상대감소료0.30 mm,산생+0.88 D적상대원시,차이균유통계학의의(P<0.001).S조여FDM지간굴광도수적차이무통계학의의;이안축변화유통계학의의(S조상대연장0.08 mm,이FDM조상대연장0.29/mm).반정량PCR결과현시:P조여S조상비교,기시망막、맥락막화홍막첩상체조직M_1화M_4아형mRNA적표체차이무통계학의의(P>0.05).이재후겁부공막조직,M_1화M_4아형mRNA적표체교약물대조조현저성증가(P<0.05),기중M_1수체표체증가19.16%,M_4수체표체증가64.29%.결론 고륜서평능구유효억제돈서형각박탈근시적발전.공막조직M_1화M_4아형급기담감능통로가능삼여M수체길항제억제근시발전.
Objective To study effects of vitreous injecting M_1-selective muscarinic antagonist,pirenzepine,on expression of M_1 and M_4 receptor in retina,choroid,schera and iris-ciliary body of guinea pig with form-deprived myopia.Methods Twenty-four 1-2 week-old pigmented guinea pigs were randomly divided into four groups.Groupl:normal control (N) (n=6);group 2:simple form-deprived myopia (FDM)(n=6);group 3:drug control (S) (n=6);group 4:pirenzepine(P) (n=6).Expression changes of M_1 and M_4 muscarinic receptors at mRNA level were detected by semi-quantitative RT-PCR in retina,choroid,sclera and iris-ciliary body.Result 1.After 21 days'treatment,FDM group produced relative myopia of -4.92 D and an axial length of 0.29 mm with significance(P<0.001),compared with N group.Compared with group S,the relative refractive error in group P wag+0.88 D,and axial length decreased 0.30 mm with respectively significance(P<0.001).Differences in refractive error between grouP S and group FDM were not significant,but in axial length were significant(0.08mm vs.0.29mm)(P<0.05).2.Semi-quantitative RT-PCR:M_1 and M_4 mRNA expression showed no significant differences in the retina,choroid and iris-ciliary body of group P compared with group S(P>0.05).Of interest,in the posterior sclera,mRNA expression of group P was significantly greater than that of group S for the M_1(P< 0.05)and M_4 subtypes(P<0.05).The M_1 and M_4 subtype in group P was increased by +19.16%and +64.29% respectively. Conclusion M_1-selective muscarinic antagonist,pirenzepine,can effectively inhibit form-deprived myopia in guinea pig. M_1 and M_4 subtype in sclera and their cholinergic signaling may participate in muscarinic antagonist inhibition of myopic development.
