遗传学报
遺傳學報
유전학보
ACTA GENETICA SINICA
2003年
9期
830-834
,共5页
晏学明%任军%郭源梅%丁能水%陈克飞%高军%艾华水%陈从英%麻骏武%黄路生
晏學明%任軍%郭源梅%丁能水%陳剋飛%高軍%艾華水%陳從英%痳駿武%黃路生
안학명%임군%곽원매%정능수%진극비%고군%애화수%진종영%마준무%황로생
猪%大肠杆菌F18%FUT1基因%遗传变异
豬%大腸桿菌F18%FUT1基因%遺傳變異
저%대장간균F18%FUT1기인%유전변이
pig%E.coli F18%FUT1 gene%genetic variation
肠毒素大肠杆菌F18(ECF18)是引起仔猪断奶后水肿和腹泻病的主要病原菌,a1-岩藻糖转移酶基因(FUT1)是ECF18侵染猪小肠的受体蛋白候选基因.通过采用PCR-RFLP方法检测了5个西方商业猪种以及21个中国地方猪种(群)1458个个体在FUT1基因开放阅读框架的307核苷酸位点的G-A点突变(M307G-A)遗传变异.结果表明:5个外来猪种以及中国地方猪种中的临高猪在该FUT1基因位点存在多态性,其他中国地方猪种均表现为极端的单态分布,只有易感的GG基因型,没有多态性.由此提示:1)如果猪FUT1 M307G-A点突变是决定猪小肠上ECF18受体表达与否的关键因素,则绝大部分中国地方猪种均不具备抵抗ECF18的遗传基础,这除了表明ECF18抗性基因有可能起源于西方猪种外,同时也表明对中国地方猪种中在这个位点惟一存在多态性的海南临高猪的品种资源保存具有非常重要的意义.2)一般而言,在中国的养猪生产实践中,中国地方猪种的仔猪抗水肿与腹泻病能力普遍强于外来猪种,研究的结果提示有必要对中国地方猪种所具备的上述遗传抗性做更深入的研究,寻找、定位其相应的QTL或/和抗性基因.
腸毒素大腸桿菌F18(ECF18)是引起仔豬斷奶後水腫和腹瀉病的主要病原菌,a1-巖藻糖轉移酶基因(FUT1)是ECF18侵染豬小腸的受體蛋白候選基因.通過採用PCR-RFLP方法檢測瞭5箇西方商業豬種以及21箇中國地方豬種(群)1458箇箇體在FUT1基因開放閱讀框架的307覈苷痠位點的G-A點突變(M307G-A)遺傳變異.結果錶明:5箇外來豬種以及中國地方豬種中的臨高豬在該FUT1基因位點存在多態性,其他中國地方豬種均錶現為極耑的單態分佈,隻有易感的GG基因型,沒有多態性.由此提示:1)如果豬FUT1 M307G-A點突變是決定豬小腸上ECF18受體錶達與否的關鍵因素,則絕大部分中國地方豬種均不具備牴抗ECF18的遺傳基礎,這除瞭錶明ECF18抗性基因有可能起源于西方豬種外,同時也錶明對中國地方豬種中在這箇位點惟一存在多態性的海南臨高豬的品種資源保存具有非常重要的意義.2)一般而言,在中國的養豬生產實踐中,中國地方豬種的仔豬抗水腫與腹瀉病能力普遍彊于外來豬種,研究的結果提示有必要對中國地方豬種所具備的上述遺傳抗性做更深入的研究,尋找、定位其相應的QTL或/和抗性基因.
장독소대장간균F18(ECF18)시인기자저단내후수종화복사병적주요병원균,a1-암조당전이매기인(FUT1)시ECF18침염저소장적수체단백후선기인.통과채용PCR-RFLP방법검측료5개서방상업저충이급21개중국지방저충(군)1458개개체재FUT1기인개방열독광가적307핵감산위점적G-A점돌변(M307G-A)유전변이.결과표명:5개외래저충이급중국지방저충중적림고저재해FUT1기인위점존재다태성,기타중국지방저충균표현위겁단적단태분포,지유역감적GG기인형,몰유다태성.유차제시:1)여과저FUT1 M307G-A점돌변시결정저소장상ECF18수체표체여부적관건인소,칙절대부분중국지방저충균불구비저항ECF18적유전기출,저제료표명ECF18항성기인유가능기원우서방저충외,동시야표명대중국지방저충중재저개위점유일존재다태성적해남림고저적품충자원보존구유비상중요적의의.2)일반이언,재중국적양저생산실천중,중국지방저충적자저항수종여복사병능력보편강우외래저충,연구적결과제시유필요대중국지방저충소구비적상술유전항성주경심입적연구,심조、정위기상응적QTL혹/화항성기인.
Enterotoxigenic Escherichia coli F18(ECF18) is a main pathogen that causes edema disease and post-weaning diarrhoea in piglets,and a1-fucosytransferase(FUT1) gene has been identified as a candidate gene for controlling the expression of the receptor for ECF18 bacteria.The genetic variations at position 307 nucleotide in open reading frame of FUT1 gene in 26 pig breeds (total 1458 individuals) from 5 western commercial pig breeds and 21 Chinese native pig breeds were investigated by PCR-RFLP.The results showed that the genetic polymorphisms of the FUT1 locus were only detected in 5 western pig breeds and the Chinese Lingao pig breed,5 western pig breeds possessed 3 different genotypes,and Lingao pig breed had two susceptible genotypes GG and AG,while all the other 20 Chinese native pig breeds only presented the susceptible genotype GG.The results indicated that if M307G-A point mutation in the coding region of FUT1 gene was the key factor determining the expression of the ECF18 receptor,most of Chinese native pig breeds were absent of the genetic background on the resistance to ECF18 bacteria.In this case,it was inferred that the resistance gene to ECF18 might be originated from western pig breeds.In addition,it is of great importance for the conservation of Lingao pig breed as it is the only found Chinese native pig breed possessing resistance M307A allele in FUT1 gene.Generally,compared with exotic pig breeds,Chinese native pig breeds have stronger resistance to edema disease and post-weaning diarrhoea in piglets.The results suggested that further study should be done to identify and characterize putative QTL (quantitative trait locus) or/and the functional gene responsible for the resistance to ECF18 in Chinese native pig breeds.
