CAJ | 학술논문

WATERGATE(W3/W5)是NMR实验中使用较为广泛的溶剂峰抑制方法,但该方法通常仅能对一个溶剂信号进行有效抑制.本文对WATERGATE的脉冲序列进行修饰、优化,发展了两种新的双溶剂峰抑制方法.实验结果表明,经过修饰、优化后的WATERGATE,实验的设立非常简单,不需要复杂的优化过程就能够对双(多)溶剂峰进行有效抑制,可用于常规1D & 2D NMR 实验、HPLC-NMR实验,以及天然产物粗提取物的分析中.
WATERGATE(W3/W5)시NMR실험중사용교위엄범적용제봉억제방법,단해방법통상부능대일개용제신호진행유효억제.본문대WATERGATE적맥충서렬진행수식、우화,발전료량충신적쌍용제봉억제방법.실험결과표명,경과수식、우화후적WATERGATE,실험적설립비상간단,불수요복잡적우화과정취능구대쌍(다)용제봉진행유효억제,가용우상규1D & 2D NMR 실험、HPLC-NMR실험,이급천연산물조제취물적분석중.
Solvent signal suppression is essential for biological NMR spectroscopy. For applications using reversed-phase HPLC-NMR or studies of natural product extracts with mixed solvents, simultaneous suppression of the signals from multiple solvents is often required in order to get high quality NMR spectrum. There are a number of approaches developed for solvent signal suppression. Although most of these approaches can be used for multiple solvent signals suppression, simple methods with high suppression efficiency are still needed. In this article, double WATERGATE approach was extended for such purpose. Two simple extensions, DSS-W5 (Double Solvent Suppression WATERGATE) and GASP-W5 (GASP-based WATERGATE), were established and used to acquire one- and two-dimensional (1D & 2D) NMR spectra in HPLC-NMR and from natural product extracts, respectively. The results demonstrated that the new methods are easy to implement and applicable for routine NMR spectroscopy.