CAJ | 학술논문

为筛选具有防污活性的物质,对孔石莼进行了提取,利用海洋硅藻检测了各提取物的防污活性.首先,使用极性不同的有机溶剂对孔石莼分步提取,然后,将得到的各种粗提物加入到硅藻培养液中,通过显微镜计数硅藻附着生长数量.实验发现各提取物均明显地抑制了硅藻的附着及生长,特别是乙酸乙酯提取物在1 g/L浓度下对硅藻附着生长的抑制率达到99%以上.对硅藻生长影响程度依次为:乙酸乙酯提取物>乙醇提取物>石油醚提取物>蒸馏水提取物.
위사선구유방오활성적물질,대공석순진행료제취,이용해양규조검측료각제취물적방오활성.수선,사용겁성불동적유궤용제대공석순분보제취,연후,장득도적각충조제물가입도규조배양액중,통과현미경계수규조부착생장수량.실험발현각제취물균명현지억제료규조적부착급생장,특별시을산을지제취물재1 g/L농도하대규조부착생장적억제솔체도99%이상.대규조생장영향정도의차위:을산을지제취물>을순제취물>석유미제취물>증류수제취물.
In order to screen the antifouling active substances,Ulva pertusa Kjellm was extracted by the different solvents. The antifouling activity was tested by the settlement inhibition assay with the benthic diatoms. First,Ulva pertusa Kjellm was extracted by the different organic solvents step-by-step. Then, the crude extracts were put into the liquid medium of diatoms and the settlement of diatoms was observed by a microscope. The results showed that the crude extracts inhibited visibly the settlement and growth of diatoms. Especially the extract of ethyl acetate inhibited the settlement of diatoms by 99% at the concentration of 1 g/L. In the case of settlement inhibition assay with benthic diatoms, the relative potency of extracts in antifouling activity was: ethyl acetate>ethanol>petroleum ether>distilled water.