CAJ | 학술논문

表面等离子体谐振生物传感器是基于光学原理的生化分析系统,可以对分子间的相互作用动力学进行实时分析,并且无须对分子进行标记.该研究利用自行研制的表面等离子谐振生物传感器对特定条件下人血清白蛋白与其抗体之间的相互作用动力学进行了分析.结果表明:人血清蛋白与固相抗体之间结合速率常数为0.538 M~(-1)s~(-1),解离速率常数为7.37×10~(-3)s~(-1).相互作用动力学分析为发展微量白蛋白的检测技术奠定了基础.
표면등리자체해진생물전감기시기우광학원리적생화분석계통,가이대분자간적상호작용동역학진행실시분석,병차무수대분자진행표기.해연구이용자행연제적표면등리자해진생물전감기대특정조건하인혈청백단백여기항체지간적상호작용동역학진행료분석.결과표명:인혈청단백여고상항체지간결합속솔상수위0.538 M~(-1)s~(-1),해리속솔상수위7.37×10~(-3)s~(-1).상호작용동역학분석위발전미량백단백적검측기술전정료기출.
Surface Plasmon resonance biosensor is a kind of analysis system based on the principle of physio-optics allowing real time and label free kinetic analysis of molecular interaction.A self-developed SPR biosensor was used to study the kinetics between human serum albumin and the antibody. The associate constant is calculated to be 0.538 M~(-1)s~(-1) ,and the dissociate constant is 7.37×10~(-3)s~(-1). The study lay the foundation of the developing human serum albumin detection technology.