生物学杂志
生物學雜誌
생물학잡지
JOURNAL OF BIOLOGY
2010年
1期
39-42
,共4页
厉荣玉%钱森和%王萍%董群
厲榮玉%錢森和%王萍%董群
려영옥%전삼화%왕평%동군
白色念珠菌%RAPD反应体系%优化
白色唸珠菌%RAPD反應體繫%優化
백색념주균%RAPD반응체계%우화
Candida albicans%RAPD reaction system%optimization
建立白色念珠菌RAPD的最佳反应体系,并应用于其基因组DNA扩增.通过单因子试验分别研究了Mg~(2+)、dNTPs、Taq酶、引物和模板DNA等浓度对RAPD反应的影响;同时,应用L_(16)(4~5)正交试验研究了DNA模板、Mg~(2+)、Taq酶、dNTPs和引物浓度对RAPD反应的影响.以务带稳定、丰富、清晰为标准,获得了白色念珠菌基因组DNA的RAPD扩增优化条件;对于白色念珠菌的最适RAPD反应体系为Mg~(2+)1.5mmol/L、dNTPs 250μmol/L、引物0.6ttmol/L、模板100ng/25μL、Taq DNA聚合酶1.5U/25μL.
建立白色唸珠菌RAPD的最佳反應體繫,併應用于其基因組DNA擴增.通過單因子試驗分彆研究瞭Mg~(2+)、dNTPs、Taq酶、引物和模闆DNA等濃度對RAPD反應的影響;同時,應用L_(16)(4~5)正交試驗研究瞭DNA模闆、Mg~(2+)、Taq酶、dNTPs和引物濃度對RAPD反應的影響.以務帶穩定、豐富、清晰為標準,穫得瞭白色唸珠菌基因組DNA的RAPD擴增優化條件;對于白色唸珠菌的最適RAPD反應體繫為Mg~(2+)1.5mmol/L、dNTPs 250μmol/L、引物0.6ttmol/L、模闆100ng/25μL、Taq DNA聚閤酶1.5U/25μL.
건립백색념주균RAPD적최가반응체계,병응용우기기인조DNA확증.통과단인자시험분별연구료Mg~(2+)、dNTPs、Taq매、인물화모판DNA등농도대RAPD반응적영향;동시,응용L_(16)(4~5)정교시험연구료DNA모판、Mg~(2+)、Taq매、dNTPs화인물농도대RAPD반응적영향.이무대은정、봉부、청석위표준,획득료백색념주균기인조DNA적RAPD확증우화조건;대우백색념주균적최괄RAPD반응체계위Mg~(2+)1.5mmol/L、dNTPs 250μmol/L、인물0.6ttmol/L、모판100ng/25μL、Taq DNA취합매1.5U/25μL.
To establish the optimization of RAPD reaction system for Candidia Albicans.the genomic DNA amplification was used for test.Effects of the concentrations of different substances like Mg~(2+),dNTPs,DNA,primer,and Taq polymerase on RAPD amplification were studied through single factor experimentation.At the same time,the L_(16)(4~5)orthogonal experimentation was applied to study the effects of different concentrations of DNA,Mg~(2+),Taq polymerase,dNTPs,and primer.Reset showed the optimization of RAPD reaction system for Candidia Albicans was 1.5mmol/L Mg2~(+),250μmol/L dNTPs,0.6mol/L primer,100ng/25μL tempiate DNA,and 1.5U/25μL Taq DNA ploymerase.
