CAJ | 학술논문

[目的]比较新疆和黄河流域两生态区棉花品种的体细胞再生能力,建立高效棉花体细胞再生体系.[方法]以新疆棉区的新陆中15号、新陆中18号、系9;黄河流域棉区的中棉所35号、中棉所44号及对照品种柯字312为研究对象,优化棉花体细胞胚胎发生的相关条件参数.[结果]在IBA+KT的激素组合下,6个棉花品种再生了5个.[结论]新疆棉区和黄河流域棉花品种的植株再生潜力没有明显差别,但新疆棉花品种体细胞胚胎发生所需的时间长.新陆中15号的愈伤组织分化率及胚胎萌发率都比较高,具备作为棉花优良遗传转化受体的基本特征,目前已经用于农杆菌介导的转基因试验,为新疆棉花组织培养及转基因育种工作提供了重要基础材料.
[목적]비교신강화황하류역량생태구면화품충적체세포재생능력,건립고효면화체세포재생체계.[방법]이신강면구적신륙중15호、신륙중18호、계9;황하류역면구적중면소35호、중면소44호급대조품충가자312위연구대상,우화면화체세포배태발생적상관조건삼수.[결과]재IBA+KT적격소조합하,6개면화품충재생료5개.[결론]신강면구화황하류역면화품충적식주재생잠력몰유명현차별,단신강면화품충체세포배태발생소수적시간장.신륙중15호적유상조직분화솔급배태맹발솔도비교고,구비작위면화우량유전전화수체적기본특정,목전이경용우농간균개도적전기인시험,위신강면화조직배양급전기인육충공작제공료중요기출재료.
[Objective and Method]In this study 6 genotypes of upland cotton were selected for comparison of their regenerability,including 2 cultivars from Yellow River valley, 3 cultivars from Xinjiang,using a highly responsible genotypes Coker 312 as a material. [Result]Most of the genotypes showed high regenerability under the IBA/KT hormone regime.[Conclusion]Under the optimized culture procedure, most of the genotypes collected from the two planting areas showed the similar regeneable abilities.The total number of embryos and frequency of embryo germination of "XLZ15"was higher for cotton transformation. This study will improve the transgenic efficiency in Xinjiang.