CAJ | 학술논문

为了解舟山沿海贝类产品中副溶血性弧菌污染情况,我们于2008年3个不同季节,从不同的农贸市场采集贝类标本,采用实时荧光定量PCR检测方法和常规培养方法,对舟山沿海贝类产品中的副溶血性弧菌进行了检测,同时对分离到的菌株进行血清学分型和毒力基因检测.结果60份贝类产品中,采用常规的分离培养方法,阳性率为83.33%,而采用实时荧光定量PCR检测方法,全部检出副溶血性弧菌.对分离到的50株副溶血性弧菌进行耐热溶血素(tdh)基因检测,结果4株tdh阳性.监测结果表明舟山贝类海产品中携带副溶血性弧菌情况比较严重,应引起足够的重视.
위료해주산연해패류산품중부용혈성호균오염정황,아문우2008년3개불동계절,종불동적농무시장채집패류표본,채용실시형광정량PCR검측방법화상규배양방법,대주산연해패류산품중적부용혈성호균진행료검측,동시대분리도적균주진행혈청학분형화독력기인검측.결과60빈패류산품중,채용상규적분리배양방법,양성솔위83.33%,이채용실시형광정량PCR검측방법,전부검출부용혈성호균.대분리도적50주부용혈성호균진행내열용혈소(tdh)기인검측,결과4주tdh양성.감측결과표명주산패류해산품중휴대부용혈성호균정황비교엄중,응인기족구적중시.
To evaluate the contamination status of Vibrio parahaemolyticus in seashells in Zhoushan coastal area, a total of 60 fresh seashells samples were collected from different Zhoushan's retail markets and detected for Ⅴ. parahaemolyticus by real-time quantitative PCR and cultivation method. The serotype and tdh genes were also tested. The results showed that the contaimination of Ⅴ. parahaemolyticus was 83.33 % by cultivation method, and 100 % by real-time quantitative PCR. The themostale direct hemolysin (tdh) genes were detected in 4 out of 50 isolates of Ⅴ. parahaemolyticus. Heavy Ⅴ. parahaemolyticus contamination was evident in seashells in Zhoushan.