中国粮油学报
中國糧油學報
중국량유학보
JOURNAL OF THE CHINESE CEREALS AND OILS ASSOCIATION
2010年
2期
23-30
,共8页
韦志彦%王金水%张艳%李兴军
韋誌彥%王金水%張豔%李興軍
위지언%왕금수%장염%리흥군
小麦%后熟%蛋白质%氨基酸%谷蛋白%硬麦%软麦
小麥%後熟%蛋白質%氨基痠%穀蛋白%硬麥%軟麥
소맥%후숙%단백질%안기산%곡단백%경맥%연맥
wheat%post-maturation%protein%amino acid%glutenin%soft wheat%hard wheat
分析了储存在4℃和室温条件下新收获的软麦和硬麦在后熟过程中的生理生化变化.新收获小麦在20℃的发芽率超过80%,在收获后3~4个月中呼吸有2次高峰,尤其第1个呼吸峰非常明显,室温条件较4 ℃提高第1个呼吸峰值.当呼吸速率趋于不变时,软麦硬度增大在第4个月,硬麦在第5个月.水溶蛋白、盐溶蛋白及谷蛋白各有2次合成高峰.硬麦醇溶蛋白在第60天有一个积累高峰,软麦至少有2个积累高峰,分别在第30天和60天.谷蛋白合成最高峰在第90天,与水溶蛋白类似,而醇溶蛋白合成最高峰在第60天,与盐溶蛋白类似.随着醇溶蛋白和游离巯基含量减少,谷蛋白迅速积累.常温条件下谷蛋白的氧化聚合较低温条件下迅速,积累量大.软麦谷蛋白氧化聚合过程较硬麦早.谷蛋白积累之前,绝大部分氨基酸急剧积累,在室温条件较4℃下积累明显.半胱氨酸含量在90~120天时增加到最大,之后迅速减少.
分析瞭儲存在4℃和室溫條件下新收穫的軟麥和硬麥在後熟過程中的生理生化變化.新收穫小麥在20℃的髮芽率超過80%,在收穫後3~4箇月中呼吸有2次高峰,尤其第1箇呼吸峰非常明顯,室溫條件較4 ℃提高第1箇呼吸峰值.噹呼吸速率趨于不變時,軟麥硬度增大在第4箇月,硬麥在第5箇月.水溶蛋白、鹽溶蛋白及穀蛋白各有2次閤成高峰.硬麥醇溶蛋白在第60天有一箇積纍高峰,軟麥至少有2箇積纍高峰,分彆在第30天和60天.穀蛋白閤成最高峰在第90天,與水溶蛋白類似,而醇溶蛋白閤成最高峰在第60天,與鹽溶蛋白類似.隨著醇溶蛋白和遊離巰基含量減少,穀蛋白迅速積纍.常溫條件下穀蛋白的氧化聚閤較低溫條件下迅速,積纍量大.軟麥穀蛋白氧化聚閤過程較硬麥早.穀蛋白積纍之前,絕大部分氨基痠急劇積纍,在室溫條件較4℃下積纍明顯.半胱氨痠含量在90~120天時增加到最大,之後迅速減少.
분석료저존재4℃화실온조건하신수획적연맥화경맥재후숙과정중적생리생화변화.신수획소맥재20℃적발아솔초과80%,재수획후3~4개월중호흡유2차고봉,우기제1개호흡봉비상명현,실온조건교4 ℃제고제1개호흡봉치.당호흡속솔추우불변시,연맥경도증대재제4개월,경맥재제5개월.수용단백、염용단백급곡단백각유2차합성고봉.경맥순용단백재제60천유일개적루고봉,연맥지소유2개적루고봉,분별재제30천화60천.곡단백합성최고봉재제90천,여수용단백유사,이순용단백합성최고봉재제60천,여염용단백유사.수착순용단백화유리구기함량감소,곡단백신속적루.상온조건하곡단백적양화취합교저온조건하신속,적루량대.연맥곡단백양화취합과정교경맥조.곡단백적루지전,절대부분안기산급극적루,재실온조건교4℃하적루명현.반광안산함량재90~120천시증가도최대,지후신속감소.
The physiological change of newly harvested soft wheat and hard wheat variety grain samples stored at room temperature and 4 ℃ for 3~4 months were studied. Results : The germination rates at20 ℃ of seven newly harvest wheat varieties are over 80%. Both soft wheat and hard wheat grains present two peaks of respiration, and the first respiration peak is higher for storage at room temperature than at 4 ℃. When grain respiration trends stable, grain hardness increase occurs at the 5th month for hard wheat and at the 4th month for soft wheat. The accumulations of water-soluble protein, salt-soluble protein and glutenin exhibit two peaks. The peak accumulation of alcoholsoluble protein appears on the 60th day after harvest for hard wheat, while two peaks for soft wheat appear on the 30th day and 60th day, respectively. The highest peak for glutenin accumulation on the 90th day is similar to that of water-soluble protein. The highest accumulations for both alcohol-soluble protein and salt-soluble protein are on the 60th day. The glutenin content increases quickly when both alcohol-soluble protein and free SH contents decrease, especially at room temperature. The oxidative polymerization of glutenin for soft wheat is earlier than for hard wheat. Most amino acids accumulate before glutenin accumulation, and the amino acid accumulation is more evident at room temperature than at 4 ℃. Cysteine increases to a peak value during the 90~120 th day, and then decreases.