CAJ | 학술논문

各种植物对NaCl都具有一定的敏感性.本实验利用野生型百脉根组织培养再生苗为研究材料,在附加不同浓度NaCl的MS培养基上观察其生长及生根状态,确定百脉根试管苗对NaCl的临界耐受能力,目的是为筛选转耐盐基因百脉根抗盐性植株提供实验依据,同时也可以初步检测所克隆基因的耐盐效果.实验结果:NaCl浓度为0.7%以上的处理,试管苗叶片发白,长势弱,不生根或根停止伸长变褐;NaCl浓度为0.7%(不包括0.7%)以下的处理中,试管苗叶片绿色,长势旺盛,根系较多,与对照(NaCl浓度为0)试管苗状态接近.
각충식물대NaCl도구유일정적민감성.본실험이용야생형백맥근조직배양재생묘위연구재료,재부가불동농도NaCl적MS배양기상관찰기생장급생근상태,학정백맥근시관묘대NaCl적림계내수능력,목적시위사선전내염기인백맥근항염성식주제공실험의거,동시야가이초보검측소극륭기인적내염효과.실험결과:NaCl농도위0.7%이상적처리,시관묘협편발백,장세약,불생근혹근정지신장변갈;NaCl농도위0.7%(불포괄0.7%)이하적처리중,시관묘협편록색,장세왕성,근계교다,여대조(NaCl농도위0)시관묘상태접근.
Most plants are sensitive to NaCl.In order to identify the NaCl tolerance of Lotus corniculatus L.regenerants in vitro,the growth and rooting of these regenerants cultured in the MS medium supplemented with different concentration NaCl were determined in the experiment.The results showed that plants grew slowly and could not rooting.their leaves turned white when NaCl concentration was above 0.7%.When NaCl concentration was below 0.7%,the plants grew as same as the control.This method could be used in identifying salt tolerance of transgenic plants.