CAJ | 학술논문

讨论了皮肤外用益智油对紫外线（UV）照射引起的小鼠光老化皮肤的影响.实验采用清洁级雌性昆明种小鼠28只,随机分成4组：正常对照组（I组）、模型组（II组）、模型＋外涂5%益智雪花膏组（III组）、模型＋外涂雪花膏组（IV组）,每组7只.模拟日光紫外线长期照射,形成皮肤光老化小鼠模型.用组织切片HE染色,观察皮肤结构改变.以生化分析方法测定羟脯氨酸（HYP）含量、超氧化物歧化酶（SOD）活力以及丙二醛（MDA）的含量.结果表明：与II组相比,III组和IV组的HYP含量、SOD活力明显升高（P〈0.05）,MDA含量明显降低（P〈0.05）;与IV组相比,III组的HYP含量、SOD活力较高,MDA含量较低.显微镜观察真皮层,III组和IV组均有不同程度的胶原纤维组织增生及多少不等的炎细胞浸润,但III组纤维细胞分化更为成熟,说明细胞修复功能增强.因此,益智油雪花膏对紫外线照射引起的小鼠皮肤光老化具有保护作用.
토론료피부외용익지유대자외선（UV）조사인기적소서광노화피부적영향.실험채용청길급자성곤명충소서28지,수궤분성4조：정상대조조（I조）、모형조（II조）、모형＋외도5%익지설화고조（III조）、모형＋외도설화고조（IV조）,매조7지.모의일광자외선장기조사,형성피부광노화소서모형.용조직절편HE염색,관찰피부결구개변.이생화분석방법측정간포안산（HYP）함량、초양화물기화매（SOD）활력이급병이철（MDA）적함량.결과표명：여II조상비,III조화IV조적HYP함량、SOD활력명현승고（P〈0.05）,MDA함량명현강저（P〈0.05）;여IV조상비,III조적HYP함량、SOD활력교고,MDA함량교저.현미경관찰진피층,III조화IV조균유불동정도적효원섬유조직증생급다소불등적염세포침윤,단III조섬유세포분화경위성숙,설명세포수복공능증강.인차,익지유설화고대자외선조사인기적소서피부광노화구유보호작용.
To investigate the influence of topical use of Miquel oxyphylla cream on the ultraviolet（UV）radiation in duced photoaging skin in mice.Twenty-eight female mice were randomly divided into four group of normal control（group I）,photoaging mode（group Ⅱ）,model plus Miquel oxyphylla-5%cream（group Ⅲ）and model plus cream（group IV）with 7 mice each.The mouse model of photoaging skin was established by long-term UV radiation.Histologi cal sections stained with HE,and the structural changes in skin was observed.HYP,SOD,MDA in the murine skin were detected by biochemical method.The result showed that Compared with group Ⅱ,HYP,SOD in group Ⅲ and group IV increased significantly（P0.05）,MDA decreased significantly（P0.05）.Compared with group IV,HYP,SOD in group Ⅲ were higher than that of in group IV,the content of MDA was lower than that of in group IV.There were obvious fibro blasts hyperplasia in group Ⅲ and group IV,but the fiber cell of group Ⅲ differentiated more mature.That indicates the repair function of group Ⅲ was stronger than group IV.Miquel oxyphylla cream has a protective effect against photoaging.