CAJ | 학술논문

目的研究唾液作为日本血吸虫病诊断与筛查标本的可行性.方法建立日本血吸虫兔感染模型,收集兔和慢性血吸虫病人成对的唾液与血清标本,以间接ELIS A检测唾液、血清标本中的抗血吸虫特异性抗体.结果①兔唾液与血清样本抗体检测的特异性分别为93.33%(28/30)和96.67% (29/30),感染兔血清与唾液样本的抗体检测敏感性分别为100%(24/24)和87.5% (21/24).血清与唾液中的抗SEA (可溶性虫卵抗原) IgG水平具显著相关性(r=0.5307, P=0.0038<0.05), 与血清标本相同,唾液中抗SEA IgG水平可反映兔感染和治疗状况.②病人唾液抗体检测的敏感性为90.62% (29/32),血清抗体检测的敏感性为100% (32/32).正常人唾液140份,阳性8份(即假阳性率5.75%),正常人血清156份,阳性6份(假阳性率3.84%).病人唾液与血清中的特异性抗体含量具显著相关性(r=0.4227, P=0.008<0.05).结论唾液中的抗日本血吸虫特异性抗体的检测可作为非损伤性的血吸虫病免疫诊断和筛查方法.
목적연구타액작위일본혈흡충병진단여사사표본적가행성.방법건립일본혈흡충토감염모형,수집토화만성혈흡충병인성대적타액여혈청표본,이간접ELIS A검측타액、혈청표본중적항혈흡충특이성항체.결과①토타액여혈청양본항체검측적특이성분별위93.33%(28/30)화96.67% (29/30),감염토혈청여타액양본적항체검측민감성분별위100%(24/24)화87.5% (21/24).혈청여타액중적항SEA (가용성충란항원) IgG수평구현저상관성(r=0.5307, P=0.0038<0.05), 여혈청표본상동,타액중항SEA IgG수평가반영토감염화치료상황.②병인타액항체검측적민감성위90.62% (29/32),혈청항체검측적민감성위100% (32/32).정상인타액140빈,양성8빈(즉가양성솔5.75%),정상인혈청156빈,양성6빈(가양성솔3.84%).병인타액여혈청중적특이성항체함량구현저상관성(r=0.4227, P=0.008<0.05).결론타액중적항일본혈흡충특이성항체적검측가작위비손상성적혈흡충병면역진단화사사방법.
Objective To assess the feasibility of using saliva for Schistosomiasis japonica diagnosis.Methods Schistosoma japonicum infected animal model was established. Pairs of saliv a and serum samples from rabbits and chronic schistosomiasis patients were colle cted. Anti-schistosoma specific antibodies in saliva and serum were detected by indirect ELISA.Results The specificities of antibody detection of rabbit saliva and serum were 93% (28/30) and 97% (29/30), respectively, and the sensitivities of antibody detection of rabbit serum and saliva were 100% (24/24) and 88% (21/24), respectively. A significant correlation (r=0.5307, P=0.0038<0.05) existed between anti -SEA IgG levels in serum and saliva. As with those in serum, anti-SEA IgG levels in saliva could reflect the state of infection and treatment. The sensitivity of antibody detection was 91% (29/32) for patient saliva samples and 100% (32 /32) for their sera. 8 samples were positive in 140 normal saliva samples (I.e . 6% false positive rate) and 6 samples were positive in 156 normal serum sampl es (4% false positive rate). There was a significant correlation (r=0.4227 , P=0.008<0.05) between specific antibodies in saliva and serum.Conclusion The detection of specific antibodies in saliva can be used as a non-invasive im munodiagnosis method of Schistosomiasis japonica.