中国行为医学科学
中國行為醫學科學
중국행위의학과학
2008年
9期
789-791
,共3页
陆佳静%王晓岚%姚文杰%袁耀萍
陸佳靜%王曉嵐%姚文傑%袁耀萍
륙가정%왕효람%요문걸%원요평
白首乌%血管性痴呆%Morris水迷宫%学习记忆%抗氧化
白首烏%血管性癡呆%Morris水迷宮%學習記憶%抗氧化
백수오%혈관성치태%Morris수미궁%학습기억%항양화
Cynanchum auriculatum%Vascular dementia%Morris water-maze%Learning-memoy%Anti-oxidant
目的 研究自首乌提取物对血管性痴呆模型小鼠学习记忆功能和抗氧化水平的影响.方法 小鼠反复脑缺血再灌注合并尾部放血降压建立痴呆模型;Morris水迷宫观察各组小鼠的学习记忆功能;快速断头处死,观测张口喘气时间;测定海马区及大脑皮质组织匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)水平.结果 与模型组相比,药物高剂量组逃避潜伏期缩短,差异具有显著性(P<0.01),正确探索时间延长(P<0.01);张口喘气时间延长[(19.3±1.6)s vs(15.7±0.7)s,P<0.01];SOD活性升高[(121.0±14.2)U·mgprot-1 vs(101.5±9.4)U·mgprot-1,P<0.05];MDA含量降低[(5.12±1.09)nmol·mgprot-1和(6.77±0.96)nmol·mgprot-1,P<0.01].结论 白首乌提取物对小鼠脑缺血再灌注损伤有保护作用,其机制可能与抗自由基损伤有关.
目的 研究自首烏提取物對血管性癡呆模型小鼠學習記憶功能和抗氧化水平的影響.方法 小鼠反複腦缺血再灌註閤併尾部放血降壓建立癡呆模型;Morris水迷宮觀察各組小鼠的學習記憶功能;快速斷頭處死,觀測張口喘氣時間;測定海馬區及大腦皮質組織勻漿中超氧化物歧化酶(SOD)、丙二醛(MDA)水平.結果 與模型組相比,藥物高劑量組逃避潛伏期縮短,差異具有顯著性(P<0.01),正確探索時間延長(P<0.01);張口喘氣時間延長[(19.3±1.6)s vs(15.7±0.7)s,P<0.01];SOD活性升高[(121.0±14.2)U·mgprot-1 vs(101.5±9.4)U·mgprot-1,P<0.05];MDA含量降低[(5.12±1.09)nmol·mgprot-1和(6.77±0.96)nmol·mgprot-1,P<0.01].結論 白首烏提取物對小鼠腦缺血再灌註損傷有保護作用,其機製可能與抗自由基損傷有關.
목적 연구자수오제취물대혈관성치태모형소서학습기억공능화항양화수평적영향.방법 소서반복뇌결혈재관주합병미부방혈강압건립치태모형;Morris수미궁관찰각조소서적학습기억공능;쾌속단두처사,관측장구천기시간;측정해마구급대뇌피질조직균장중초양화물기화매(SOD)、병이철(MDA)수평.결과 여모형조상비,약물고제량조도피잠복기축단,차이구유현저성(P<0.01),정학탐색시간연장(P<0.01);장구천기시간연장[(19.3±1.6)s vs(15.7±0.7)s,P<0.01];SOD활성승고[(121.0±14.2)U·mgprot-1 vs(101.5±9.4)U·mgprot-1,P<0.05];MDA함량강저[(5.12±1.09)nmol·mgprot-1화(6.77±0.96)nmol·mgprot-1,P<0.01].결론 백수오제취물대소서뇌결혈재관주손상유보호작용,기궤제가능여항자유기손상유관.
Objective To observe the effects of Radix Cynanchum auriculatum extract in mice with vascular dementia.Methods An animal model was established through repeated cerebral ischemia-reperfusion in mice with tail bleeding hypotension.Morris water-mase was used to determine the learning and memory ability；gasping time of mice by decapitation were evaluated.Changes in superoxide dimutase(SOD)，malonaldehyde(MDA)were observed by means of spectro photometry.Results To contrast high dose drug group with model group，the escape latencies were significantly shorter(P＜0.01)，contrarily the time in correct zone were prolonged(P＜0.01).And the gasping time of mice by decapitation were also protracted[(19.3±1.6)s vs(15.7±0.7)s](P＜0.01).The high dose group had less content of MDA[(5.12±1.09)nmol·mgprot-1 vs(6.77±0.96)nmol·mgprot-1](P＜0.01)，but more activity of SOD in hippocampus and cerebral cortex[(121.0±14.2)U·mgprot-1 vs(101.5±9.4)U·mgprot-1](P＜0.05).Conclusion Radix Cynanchum auriculatum extract has protective effect on cerebral ischemia-reperfusion injury.The mechanism may be related with anti-oxidation and free radical.
