中华结核和呼吸杂志
中華結覈和呼吸雜誌
중화결핵화호흡잡지
Chinese Journal of Tuberculosis and Respiratory Diseases
2011年
12期
923-928
,共6页
刘轾彬%郑瑞娟%肖和平%沙巍%张青%吴福蓉%孙华%张忠顺%崔海燕%刘一典%唐神结
劉輊彬%鄭瑞娟%肖和平%沙巍%張青%吳福蓉%孫華%張忠順%崔海燕%劉一典%唐神結
류지빈%정서연%초화평%사외%장청%오복용%손화%장충순%최해연%류일전%당신결
疾病遗传易感性%多态性,单核苷酸%结核%疾病特征
疾病遺傳易感性%多態性,單覈苷痠%結覈%疾病特徵
질병유전역감성%다태성,단핵감산%결핵%질병특정
Genetic predisposition to disease%Polymorphism,single nucleotide%Tuberculosis%Disease attributes
目的 探讨汉族人群结核病易感基因多态性与临床特征的相关性.方法 收集2007年1月至2008年12月上海市肺科医院收治的459例汉族结核病住院患者,分析结核病患者的临床特征(性别、发热、病灶范围、空洞、咯血、初治或复治),采用多种单核苷酸多态性分型技术,检测其维生素D受体(VDR)基因Fok Ⅰ位点和Taq Ⅰ位点,自然抗性相关巨噬细胞蛋白1(NRAMP1)基因D543N、INT4和3'UTR位点,甘露糖结合凝集素(MBL)基因HL、YX和QP位点,以及γ-干扰素编码(IFNG)基因874AT位点,并对上述基因进行分型.采用单因素方差分析检验基因多态性与临床特征的相关性.结果 结核病发热患者VDR基因Fok Ⅰ位点CC、CT和TT基因型的变异率分别为54.7％ (29/53)、13.2％ (7/53)和32.1％ (17/53),不发热患者分别为40.6％( 52/128)、30.5％ (39/128)和28.9％ (37/128),差异有统计学意义(x2 =6.183,P＜0.05);CT基因型患者出现发热症状的比例为15.2％ (7/46),非CT基因型患者出现发热症状的比例为34.1％ (46/135),差异有统计学意义(x2 =5.891,P＜0.05),提示CT基因型患者更少出现发热症状；MBL基因QP位点的TT+TC和CC基因型变异率,结核病初治患者分别为28.3％ (60/212)和71.7％( 152/212),复治患者分别为19.1％(41/215)和80.9％ (174/215),差异有统计学意义(x2=5.038,P＜0.05).其余各位点基因型与结核病临床特征均无相关性(x2值为0.001～2.732,均P＞0.05).结论 VDR基因Fok Ⅰ位点多态性可能与结核病发热症状具有相关性,其中CT变异对结核病患者发热可能具有保护作用,MBL基因QP位点多态性可能与结核病复发有相关性.
目的 探討漢族人群結覈病易感基因多態性與臨床特徵的相關性.方法 收集2007年1月至2008年12月上海市肺科醫院收治的459例漢族結覈病住院患者,分析結覈病患者的臨床特徵(性彆、髮熱、病竈範圍、空洞、咯血、初治或複治),採用多種單覈苷痠多態性分型技術,檢測其維生素D受體(VDR)基因Fok Ⅰ位點和Taq Ⅰ位點,自然抗性相關巨噬細胞蛋白1(NRAMP1)基因D543N、INT4和3'UTR位點,甘露糖結閤凝集素(MBL)基因HL、YX和QP位點,以及γ-榦擾素編碼(IFNG)基因874AT位點,併對上述基因進行分型.採用單因素方差分析檢驗基因多態性與臨床特徵的相關性.結果 結覈病髮熱患者VDR基因Fok Ⅰ位點CC、CT和TT基因型的變異率分彆為54.7％ (29/53)、13.2％ (7/53)和32.1％ (17/53),不髮熱患者分彆為40.6％( 52/128)、30.5％ (39/128)和28.9％ (37/128),差異有統計學意義(x2 =6.183,P＜0.05);CT基因型患者齣現髮熱癥狀的比例為15.2％ (7/46),非CT基因型患者齣現髮熱癥狀的比例為34.1％ (46/135),差異有統計學意義(x2 =5.891,P＜0.05),提示CT基因型患者更少齣現髮熱癥狀；MBL基因QP位點的TT+TC和CC基因型變異率,結覈病初治患者分彆為28.3％ (60/212)和71.7％( 152/212),複治患者分彆為19.1％(41/215)和80.9％ (174/215),差異有統計學意義(x2=5.038,P＜0.05).其餘各位點基因型與結覈病臨床特徵均無相關性(x2值為0.001～2.732,均P＞0.05).結論 VDR基因Fok Ⅰ位點多態性可能與結覈病髮熱癥狀具有相關性,其中CT變異對結覈病患者髮熱可能具有保護作用,MBL基因QP位點多態性可能與結覈病複髮有相關性.
목적 탐토한족인군결핵병역감기인다태성여림상특정적상관성.방법 수집2007년1월지2008년12월상해시폐과의원수치적459례한족결핵병주원환자,분석결핵병환자적림상특정(성별、발열、병조범위、공동、각혈、초치혹복치),채용다충단핵감산다태성분형기술,검측기유생소D수체(VDR)기인Fok Ⅰ위점화Taq Ⅰ위점,자연항성상관거서세포단백1(NRAMP1)기인D543N、INT4화3'UTR위점,감로당결합응집소(MBL)기인HL、YX화QP위점,이급γ-간우소편마(IFNG)기인874AT위점,병대상술기인진행분형.채용단인소방차분석검험기인다태성여림상특정적상관성.결과 결핵병발열환자VDR기인Fok Ⅰ위점CC、CT화TT기인형적변이솔분별위54.7％ (29/53)、13.2％ (7/53)화32.1％ (17/53),불발열환자분별위40.6％( 52/128)、30.5％ (39/128)화28.9％ (37/128),차이유통계학의의(x2 =6.183,P＜0.05);CT기인형환자출현발열증상적비례위15.2％ (7/46),비CT기인형환자출현발열증상적비례위34.1％ (46/135),차이유통계학의의(x2 =5.891,P＜0.05),제시CT기인형환자경소출현발열증상；MBL기인QP위점적TT+TC화CC기인형변이솔,결핵병초치환자분별위28.3％ (60/212)화71.7％( 152/212),복치환자분별위19.1％(41/215)화80.9％ (174/215),차이유통계학의의(x2=5.038,P＜0.05).기여각위점기인형여결핵병림상특정균무상관성(x2치위0.001～2.732,균P＞0.05).결론 VDR기인Fok Ⅰ위점다태성가능여결핵병발열증상구유상관성,기중CT변이대결핵병환자발열가능구유보호작용,MBL기인QP위점다태성가능여결핵병복발유상관성.
Objective To study the correlation between polymorphisms of genes with susceptibility to tuberculosis and the clinical characteristics of tuberculosis in Han population.Methods Four hundred and fifty-nine tuberculosis inpatients of Han population in Shanghai Pulmonary Hospital from Jan 2007 to Dec 2008 were recruited.The clinical characteristics of tuberculosis ( gender,fever,extent of lesions,cavity formation,hemoptysis,initial treatment and retreatment) were observed.The polymorphisms of VDR gene (variants in Fok Ⅰ and Taq 1 ),NRAMP1 gene (variants in INT4,D543N and 3 UTR),MBL gene (variants in HL,YX and QP) and IFNG gene (variants in 874AT) were genotyped by a variety of SNP genotyping techniques.The correlation between polymorphisms of genes with susceptibility to tuberculosis and the clinical characteristics of the disease was analyzed by ANOVAs.Results The frequency of CC,CT and TT variants of Fok Ⅰ in VDR gene in cases with fever were 54.7％ (29/53),13.2％ (7/53) and 32.1％ ( 17/53),respectively,compared to40.6％ (52/128),30.5％ (39/128) and 28.9％ (37/128)in cases without fever,the difference being significant ( x2 =6.183,P ＜ 0.05 ).In patients with CT variants,15.2％ (7/46) had fever,while in patients with non-CT variants,34.1％ (46/135) had fever (x2 =5.891,P ＜ 0.05),suggesting that patients with CT variants were less likely to have fever.The frequencies of TT +TC and CC variants of QP in the MBL gene in initial treatment cases were 28.3％ (60/212) and 71.7％ ( 152/212),respectively,compared to 19.1％ (41/215) and 80.9％ (174/215) in retreatment cases,the difference being significant ( x2 =5.038,P ＜ 0.05 ).No significant correlation was observed between the other variants and the clinical characteristics of tuberculosis ( x2 =0.001 - 2.732,P ＞0.05 ).Conclusions The polymorphisms of Fok Ⅰ in VDR gene was associated with fever among the clinical characteristics of tuberculosis,and patients with CT variants might be protected from fever.The polymorphisms of QP in MBL gene might be associated with recurrence of tuberculosis.
