国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2009年
2期
73-77
,共5页
饶华祥%侯玉英%于娟%刘智深%赵晋英%杨瑞%赵云鹤%郭剑津%张淑萍%张杰%侯丽萍
饒華祥%侯玉英%于娟%劉智深%趙晉英%楊瑞%趙雲鶴%郭劍津%張淑萍%張傑%侯麗萍
요화상%후옥영%우연%류지심%조진영%양서%조운학%곽검진%장숙평%장걸%후려평
刚地弓形虫%胎盘%细胞凋亡%半胱氨酸天冬氨酸蛋白酶
剛地弓形蟲%胎盤%細胞凋亡%半胱氨痠天鼕氨痠蛋白酶
강지궁형충%태반%세포조망%반광안산천동안산단백매
Toxoplasma gondii%Placenta%Apoptosis%Caspase
目的探讨孕中期弓形虫感染对BALB/c孕鼠胎盘细胞凋亡的影响.方法在小鼠妊娠第8天采用腹腔注射速殖子法建立弓形虫感染的动物模型,于妊娠第12、14、16、18天分批处死孕鼠,采用流式细胞技术检测胎盘细胞凋亡率,分光光度法测定半胱氨酸天冬氨酸蛋白酶-3(caspase-3)的活性.结果随着妊娠天数的增加,实验组和正常埘照组胎盘细胞凋亡率及caspase-3的活力均在升高,差异均具有统计学意义(P＜0.01);在相同时间,实验组的凋亡率以及酶活力均高于正常对照组,除第18天组胎盘caspase-3活力单位外,其余各组比较差异均具有统计学意义(P＜0.05).结论孕中期弓形虫感染可导致孕鼠胎盘细胞捌亡明显增加.
目的探討孕中期弓形蟲感染對BALB/c孕鼠胎盤細胞凋亡的影響.方法在小鼠妊娠第8天採用腹腔註射速殖子法建立弓形蟲感染的動物模型,于妊娠第12、14、16、18天分批處死孕鼠,採用流式細胞技術檢測胎盤細胞凋亡率,分光光度法測定半胱氨痠天鼕氨痠蛋白酶-3(caspase-3)的活性.結果隨著妊娠天數的增加,實驗組和正常塒照組胎盤細胞凋亡率及caspase-3的活力均在升高,差異均具有統計學意義(P＜0.01);在相同時間,實驗組的凋亡率以及酶活力均高于正常對照組,除第18天組胎盤caspase-3活力單位外,其餘各組比較差異均具有統計學意義(P＜0.05).結論孕中期弓形蟲感染可導緻孕鼠胎盤細胞捌亡明顯增加.
목적탐토잉중기궁형충감염대BALB/c잉서태반세포조망적영향.방법재소서임신제8천채용복강주사속식자법건립궁형충감염적동물모형,우임신제12、14、16、18천분비처사잉서,채용류식세포기술검측태반세포조망솔,분광광도법측정반광안산천동안산단백매-3(caspase-3)적활성.결과수착임신천수적증가,실험조화정상시조조태반세포조망솔급caspase-3적활력균재승고,차이균구유통계학의의(P＜0.01);재상동시간,실험조적조망솔이급매활력균고우정상대조조,제제18천조태반caspase-3활력단위외,기여각조비교차이균구유통계학의의(P＜0.05).결론잉중기궁형충감염가도치잉서태반세포팔망명현증가.
Objective To investigate the placental apoptosis of BALB/c mice infected with Toxoplasma gondii during the second trimester of pregnancy.Methods The animal infection model was established by intraperitoneal injection(i.p.)of T.gondii tachyzoites at the 8th day of pregnancy in mice,then the placentas were collected at the 12th,14th,16th,18th day of pregnancy respectively.The apoptosis rate and the caspase-3 activity were determined by flow cytometry(FCM)and spectrophotometry respectively.Results Both the apoptosis rate and caspase-3 activity elevated along with the increase of the pregnant days in both the experimental group and the normal control group with significant differences(P＜0.01),both the apoptosis rate and casptrse-3 activity of the experimental group were higher than that of the normal control group at the same pregnant days,with significant differences(P＜0.05)except that of the caspase-3 activity units at the 18th day.Conclusion The placental apoptosis of pregnant mice infected with T. gondii during the second trimester of pregnancy increased significantly compared with that of normal control mice.
