CAJ | 학술논문

24只Wistar大鼠分为正常对照组、限食组、追赶生长组,检测所有大鼠血糖、血脂、血清胃泌素,内脏脂肪体脂比、脂肪细胞CCK2R mRNA和蛋白水平.结果显示限食组和正常组相比,血清胃泌素水平降低54%(P＜0.05),内脏脂肪体脂比减少55%(P＜0.05),脂肪细胞CCK2R mRNA和蛋白表达下降(2.19±0.18对3.2±0.24,0.11±0.03对0.15±0.04,P＜0.05).追赶生长组血清胃泌素水平分别高于限食组72%和正常组31%(P＜0.05),内脏脂肪体脂比高于限食组114%(P＜0.05),达到正常对照组水平;同时脂肪细胞CCK2R mRNA和蛋白表达高于正常对照组(4.09±0.59对3.2±0.24,0.25±0.05对0.15±0.04,P＜0.05).追赶生长大鼠内脏脂肪优先沉积的机制可能和胃泌素分泌增加及脂肪细胞CCK2R表达增加相关.
24지Wistar대서분위정상대조조、한식조、추간생장조,검측소유대서혈당、혈지、혈청위비소,내장지방체지비、지방세포CCK2R mRNA화단백수평.결과 현시한식조화정상조상비,혈청위비소수평강저54%(P＜0.05),내장지방체지비감소55%(P＜0.05),지방세포CCK2R mRNA화단백표체하강(2.19±0.18대3.2±0.24,0.11±0.03대0.15±0.04,P＜0.05).추간생장조혈청위비소수평분별고우한식조72%화정상조31%(P＜0.05),내장지방체지비고우한식조114%(P＜0.05),체도정상대조조수평;동시지방세포CCK2R mRNA화단백표체고우정상대조조(4.09±0.59대3.2±0.24,0.25±0.05대0.15±0.04,P＜0.05).추간생장대서내장지방우선침적적궤제가능화위비소분비증가급지방세포CCK2R표체증가상관.
Wistar rats(n=24) were divided into normal control group(NC), food restriction group(FR), and catch-up group(RN). Serum glucose,lipids, gastrin, the ratio of visceral fat to body fat, adipocyte CCK2R mRNA and protein levels were determined. Compared with NC group, FR rats had lower serum gastrin and visceral fat formation. The adipocyte CCK2R mRNA and protein levels of FR rats were lower than those of NC rats. Serum gastrin level of RN rats was higher than those of FR and NC rats(P＜0.05). The ratio of visceral fat to body fat in RN rats increased compared with FR rats and was close to that of NC rats. The adipocyte CCK2R mRNA and protein levels of RN rats were higher than those of FR and NC rats. Gastrin and its receptor pathway possibly play a role in the mechanism of visceral fat accumulation in catch-up rats.