中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2010年
4期
248-251
,共4页
增生性瘢痕%成纤维细胞%水蛭素
增生性瘢痕%成纖維細胞%水蛭素
증생성반흔%성섬유세포%수질소
Hypertrophic scar%Fibroblasts%Hirudin
目的 探讨水蛭素对人增生性瘢痕成纤维细胞细胞周期的影响,为临床应用水蛭素治疗增生性瘢痕提供理论基础.方法 用消化法进行人增生性瘢痕和正常皮肤成纤维细胞的体外培养,用流式细胞仪检测不同浓度水蛭素对不同来源的成纤维细胞细胞周期的影响,用Western印迹法检测部分与细胞周期相关的蛋白(细胞周期蛋白依赖性激酶抑制蛋白p27及细胞周期蛋白E(cyclin-E)的表达.结果 随着水蛭素浓度的增加,人体正常皮肤及增生性瘢痕组织成纤维细胞G1期细胞增加而S期细胞则减少,周期蛋白依赖性激酶抑制蛋白p27的表达上调,细胞周期蛋白E的表达下调.结论 水蛭素通过影响细胞周期调控蛋白的表达,使正常皮肤和增生性瘢痕成纤维细胞的周期分布发生改变,导致细胞周期G1期阻滞(G1 phase arrest),从而抑制成纤维细胞增生,因此水蛭素对于瘢痕的防治有一定的作用.
目的 探討水蛭素對人增生性瘢痕成纖維細胞細胞週期的影響,為臨床應用水蛭素治療增生性瘢痕提供理論基礎.方法 用消化法進行人增生性瘢痕和正常皮膚成纖維細胞的體外培養,用流式細胞儀檢測不同濃度水蛭素對不同來源的成纖維細胞細胞週期的影響,用Western印跡法檢測部分與細胞週期相關的蛋白(細胞週期蛋白依賴性激酶抑製蛋白p27及細胞週期蛋白E(cyclin-E)的錶達.結果 隨著水蛭素濃度的增加,人體正常皮膚及增生性瘢痕組織成纖維細胞G1期細胞增加而S期細胞則減少,週期蛋白依賴性激酶抑製蛋白p27的錶達上調,細胞週期蛋白E的錶達下調.結論 水蛭素通過影響細胞週期調控蛋白的錶達,使正常皮膚和增生性瘢痕成纖維細胞的週期分佈髮生改變,導緻細胞週期G1期阻滯(G1 phase arrest),從而抑製成纖維細胞增生,因此水蛭素對于瘢痕的防治有一定的作用.
목적 탐토수질소대인증생성반흔성섬유세포세포주기적영향,위림상응용수질소치료증생성반흔제공이론기출.방법 용소화법진행인증생성반흔화정상피부성섬유세포적체외배양,용류식세포의검측불동농도수질소대불동래원적성섬유세포세포주기적영향,용Western인적법검측부분여세포주기상관적단백(세포주기단백의뢰성격매억제단백p27급세포주기단백E(cyclin-E)적표체.결과 수착수질소농도적증가,인체정상피부급증생성반흔조직성섬유세포G1기세포증가이S기세포칙감소,주기단백의뢰성격매억제단백p27적표체상조,세포주기단백E적표체하조.결론 수질소통과영향세포주기조공단백적표체,사정상피부화증생성반흔성섬유세포적주기분포발생개변,도치세포주기G1기조체(G1 phase arrest),종이억제성섬유세포증생,인차수질소대우반흔적방치유일정적작용.
Objective To study the biological effects of hirudin on the cell circle of fibroblasts in hypertrophic scar.Methods Fibroblasts were taken from normal skin and hypertrophic scar,and cultured in vitro with various concentrations of hirudin.Then,flow cytometry was used for observing cellcycles,and Western blot for some proteins (p27 and cyclin E) related with cell circle.Results With the increasing of the concentrations of hirudin,fibroblasts on G1 phase increased and on S phase decreased,and the expression of protein p27 increased,but that of protein cyclin E decreased.Conclusion Hirudin can influence the cell circle of fibroblasts derived from normal skin and hypertrophic scar by means of the expression of some proteins related with celI circle.Hirudin causes G1 phase arrest and inhibits the production of fibroblasts.Therefore,it seems that hypertrophic scar could be prevented and treated with hirudin.
