CAJ | 학술논문

测定和分析了连香树科(Cercidiphyllaceae)、交让木科(Daphniphyllaceae)、金缕梅科(Hamamelidaceae)代表植物的叶绿体matK序列(5'端3lbps除外),以木兰属作为外类群,应用邻接法构建分子系统树,结果表明:连香树科与水青树科的亲缘关系较远.连香树科、交让木科和金缕梅科形成了一个自展数据支持率(bootstrap)为100%的单系类群,其中金缕梅科枫香属(Liquidambar)、红花荷属(Rhodoleia)和金缕梅属(Hamamelis)虽构成了一个单系类群,但自展数据支持率仅为68%;连香树科与交让木科构成的单系分支自展数据支持率仅为53%.由于连香树科、交让木科、金缕梅科之间的进化距离相当短,表明这3个科之间亲缘关系密切,内部分支的自展数据支持率不高,表明它们之间准确的亲缘关系有待进一步研究.本研究结果与rbcL、aptB、18S rDNA序列分析结果相似,但自展数据支持率更高,表明matK序列分析可应用于较高等级分类群系统发育关系的研究.
측정화분석료련향수과(Cercidiphyllaceae)、교양목과(Daphniphyllaceae)、금루매과(Hamamelidaceae)대표식물적협록체matK서렬(5'단3lbps제외),이목란속작위외류군,응용린접법구건분자계통수,결과표명:련향수과여수청수과적친연관계교원.련향수과、교양목과화금루매과형성료일개자전수거지지솔(bootstrap)위100%적단계류군,기중금루매과풍향속(Liquidambar)、홍화하속(Rhodoleia)화금루매속(Hamamelis)수구성료일개단계류군,단자전수거지지솔부위68%;련향수과여교양목과구성적단계분지자전수거지지솔부위53%.유우련향수과、교양목과、금루매과지간적진화거리상당단,표명저3개과지간친연관계밀절,내부분지적자전수거지지솔불고,표명타문지간준학적친연관계유대진일보연구.본연구결과여rbcL、aptB、18S rDNA서렬분석결과상사,단자전수거지지솔경고,표명matK서렬분석가응용우교고등급분류군계통발육관계적연구.
To further resolve the phylogenetic affinities of Cercidiphyllaceae, matK gene sequences generated forTetracentraceae, Daphniphyllaceae, Cercidiphyllaceae and Liquidambar, Rhodoleia and Hamamelis of Hamamelidaceae, wefeanalyzed using Neighbor-Joining method with Magnolia as an outgroup. The matK consensus tree displays similar topology tothose of rbcL, atpB, and 18S rDNA sequences analyses respectively and combined with greater phylogenetic resolution. Itshows that Cercidiphyllaceae, Daphniphylliaceae and Hamamelidaceae formed a robust clade, but relationships among them arenot resolved; the phylogenetic relationships of Tetracentraceae to Cercidiphyllaceae are not so closed as traditional treatment.Thus, several lines of evidence, derived from both the chloroplast genome and 18S rDNA sequences, suggest the presence ofconsiderably phylogenetic affinities between Cercidiphyllaceae, Daphniphyllaceae, and Hamamelidaceae, further informationon some other quickly-evolved genes are needed to resolve phylogenetic relationships among them. The study corroborates theidea that matK sequences have potential in resolving phylogeny at family level.