CAJ | 학술논문

利用籼粳交"密阳23/吉冷1号"的F2:3代200个家系作为作图群体,在14℃条件下鉴定萌发7 d、11 d、14 d和17d时低温发芽势,并利用由SSR标记构建的分子连锁图谱为基础,对不同萌发阶段的低温发芽势进行了数量性状基因座(QTLs)检测,同时进行了低温发芽势与其他耐冷性状间的相关分析.结果表明,萌发7 d时低温发芽势及其低温反应指数呈现向低发芽势和低的低温反应指数的偏态分布,而萌发11d、14 d和17 d时低温发芽势及其低温反应指数均呈现接近正态的单峰连续分布.萌发14 d时低温发芽势与其他耐冷性的相关性较萌发7 d、11d和17d时低温发芽势更为密切,与芽期耐冷性、幼苗期耐冷性、低温下幼苗生长能力和孕穗期耐冷性表现为显著或极显著的正相关.位于第2染色体RM29-RM262区间的qLVG2和第7染色体RM336-RM118区间的qLVG7-2、qCIVG7-2在萌发11d、14 d和17 d时均检测到;位于RM29-RM262区间的qCIVG2在萌发11d和14 d时均检测到,并对表型变异的贡献率随着萌发进程而逐渐增加.与低温发芽势相关的qLVG2贡献率从6.9%增加到14.2%,qLVG7-2贡献率从9.9%增加到11.2%,而与发芽势的低温反应指数相关的qCIVG2贡献率从63%增加到9.0%,qCIVG7-2贡献率从8.3%增加12.9%.这些QTL的增效等位基因均来自强耐冷亲本吉冷1号,基因作用方式主要为部分显性.
이용선갱교"밀양23/길랭1호"적F2:3대200개가계작위작도군체,재14℃조건하감정맹발7 d、11 d、14 d화17d시저온발아세,병이용유SSR표기구건적분자련쇄도보위기출,대불동맹발계단적저온발아세진행료수량성상기인좌(QTLs)검측,동시진행료저온발아세여기타내랭성상간적상관분석.결과표명,맹발7 d시저온발아세급기저온반응지수정현향저발아세화저적저온반응지수적편태분포,이맹발11d、14 d화17 d시저온발아세급기저온반응지수균정현접근정태적단봉련속분포.맹발14 d시저온발아세여기타내랭성적상관성교맹발7 d、11d화17d시저온발아세경위밀절,여아기내랭성、유묘기내랭성、저온하유묘생장능력화잉수기내랭성표현위현저혹겁현저적정상관.위우제2염색체RM29-RM262구간적qLVG2화제7염색체RM336-RM118구간적qLVG7-2、qCIVG7-2재맹발11d、14 d화17 d시균검측도;위우RM29-RM262구간적qCIVG2재맹발11d화14 d시균검측도,병대표형변이적공헌솔수착맹발진정이축점증가.여저온발아세상관적qLVG2공헌솔종6.9%증가도14.2%,qLVG7-2공헌솔종9.9%증가도11.2%,이여발아세적저온반응지수상관적qCIVG2공헌솔종63%증가도9.0%,qCIVG7-2공헌솔종8.3%증가12.9%.저사QTL적증효등위기인균래자강내랭친본길랭1호,기인작용방식주요위부분현성.
The quantitative trait loci (QTLs) for low-temperature vigor of germination (LVG) with a germination period of 7 d, 11 d,14 d, and 17 d at 14℃ was identified using F2:3 population, which included 200 individuals and lines derived from a cross of indica and japonica "Milyang 23/Jileng 1" with microsatellite markers. The correlation coefficient between LVG and other cold tolerance traits was analyzed. LVG and the cold response index for vigor of germination (CIVG) detected when the germination period was 7 d showed a continuous distribution, which was partial to lower LVG and lower CIVG in F3 lines. LVG and CIVG detected when the germination periods were 11 d, 14 d, and 17 d showed a continuous distribution near normal, which were quantitative traits controlled by multiple genes. LVG detected when the germination period was 14 d was more correlated with other cold tolerance traits than LVG detected when the germination periods were 7 d, 11 d, and 17 d, which was significantly associated with cold tolerance during the bud bursting period, the seedling stage, the booting stage, and the growing ability under cold conditions.qLVG2 located in RM29-RM262 on chromosome 2, qLVG7-2 and qCIVG7-2 located in RM336-RM118 on chromosome 7 were detected when the germination periods were 11 d, 14 d, and 17 d. qCIVG2 located in RM29-RM262 on chromosome 2 was detected when the germination periods were 11 d and 14 d. The variation is due to the observed phenotypic variation by the above QTLs,which was increased following the germination. The variation of qLVG2 related to LVG was increased from 6.9% to 14.2%. The variation ofqLVG7-2 associated with LVG was increased from 9.9% to 11.2%. The variation ofqCIVG2 correlated with CIVG was increased from 6.3% to 9.0%. The variation of qCIVG7-2 associated with CIVG was increased from 8.3% to 12.9%. These QTL alleles were obtained from the tolerant parent Jileng 1, and the gene action was most likely to be partially dominant.