CAJ | 학술논문

背景:有关磁性附着体外包裹不锈钢材料引起的细胞凋亡,经检索CNKI数据库未见报道.目的:观察磁性附着体的3种外包裹牙科金属材料钴铬合金、钛合金及奥氏体不锈钢对L-929细胞凋亡的影响.设计、时间及地点:对比观察,实验于2007-06/12在中国医科大学中心实验室完成.材料:选用对数生长期的小鼠成纤维细胞L-g29,由中国医科大学附属第一医院肿瘤研究所提供.钴铬合金由贺立氏古莎齿科有限公司提供:钛合会由日本森田公司提供;奥氏体不锈钢由中国科学院金属研究所提供.方法:将钛合金、奥氏体不锈钢和钴铬合金3种金属材料制成圆片形,放置于24孔板中,按浸提液体积与试件表面积比值为0.1 mL/cm2放入RPMI-1640培养液,即得到材料浸提液.实验分为5组:钛合金组、奥氏体不锈钢组和钴铬合金组分别将L-929细胞接种于含不同质量浓度(250,500 g/L,1 kg/L)钛金属、奥氏体不锈钢和钴铬金属的浸提液中;阴性对照组:RPMI1640培养的L-929细胞:阳性对照组:1 00 mg/L丝列霉素处理的L-929细胞.主要观察指标:流式细胞仪检测各组不同质量浓度浸提液干预24 h对L-929细胞凋亡的影响.结果:除250 g/L质量浓度钛合金与阴性对照组之间细胞凋亡率无显著差异(P>0.05)外,其他各组材料同一质量浓度或同一材料不同质量浓度之间细胞凋亡率相比,差异均有显著件意义(P<0.01).结论:3种金属材料对细胞凋亡率均有显著影响,凋亡率大小依次为:钛合金组<奥氏体不锈钢组<钴铬合金组
배경:유관자성부착체외포과불수강재료인기적세포조망,경검색CNKI수거고미견보도.목적:관찰자성부착체적3충외포과아과금속재료고락합금、태합금급오씨체불수강대L-929세포조망적영향.설계、시간급지점:대비관찰,실험우2007-06/12재중국의과대학중심실험실완성.재료:선용대수생장기적소서성섬유세포L-g29,유중국의과대학부속제일의원종류연구소제공.고락합금유하립씨고사치과유한공사제공:태합회유일본삼전공사제공;오씨체불수강유중국과학원금속연구소제공.방법:장태합금、오씨체불수강화고락합금3충금속재료제성원편형,방치우24공판중,안침제액체적여시건표면적비치위0.1 mL/cm2방입RPMI-1640배양액,즉득도재료침제액.실험분위5조:태합금조、오씨체불수강조화고락합금조분별장L-929세포접충우함불동질량농도(250,500 g/L,1 kg/L)태금속、오씨체불수강화고락금속적침제액중;음성대조조:RPMI1640배양적L-929세포:양성대조조:1 00 mg/L사렬매소처리적L-929세포.주요관찰지표:류식세포의검측각조불동질량농도침제액간예24 h대L-929세포조망적영향.결과:제250 g/L질량농도태합금여음성대조조지간세포조망솔무현저차이(P>0.05)외,기타각조재료동일질량농도혹동일재료불동질량농도지간세포조망솔상비,차이균유현저건의의(P<0.01).결론:3충금속재료대세포조망솔균유현저영향,조망솔대소의차위:태합금조<오씨체불수강조<고락합금조
BACKGROUND: There have been no reports in CNKI database addressing the cellular apoptosis caused by stainless steel materials used in the dental magnetic attachment. OBJECTIVE: To observe the effects of 3 stainless steel materials used in the dental magnetic attachment, cobalt-chromium alloy, titanium alloy, and austenitic stainless steel on L-929 cell apoptosis. DESIGN, TIME AND SETTING: A comparative observation was performed at the Central Laboratory of China Medical University between June and December 2007. MATERIALS: Mouse L-929 fibroblasts in logarithmic growth phase were provided by Institute of Tumor, First Affiliated Hospital of China Medical University, China. Cobalt-chromium (Co-Cr) alloy was purchased from Heraeus-Kulzer Corporation, China. Titanium alloy was obtained from Morita Company, Japan. Austenitio stainless steel was provided by Institute of Mental Research Chinese Academy of Sciences, China. METHODS: The leaching liquor of Co-Cr alloy, titanium alloy, and austenitic stainless steel was made by culturing 3 kinds of round-slice mental materials in 24-well plate with RPMI-1640 medium at 0.1 mL/cm2 (leaching liquor volume: specimen surface area). L-929 fibroblasts were divided into 5 groups: Co-Cr alloy, titanium alloy, austenitic stainless steel, negative control, and positive control. The Co-Cr alloy, titanium alloy, and austenitic stainless steel groups were cultured with corresponding leaching liquor (250, 500, and 1 000 g/L). Cells from the negative control and positive control groups were cultured with simple RPMI 1640 medium and 100 mg/L mitomycin-treated L-929 cells. MAIN OUTCOME MEASURES: Following 24-hour culture, the effects of 3 kinds of leaching liquor (at different concentrations) on L-929 fibroblasts were examined through the use of flow cytometer. RESULTS: There was significant difference in cellular apoptosis between Co-Cr alloy, titanium alloy, and austenitic stainless steel groups at the same concentration or between different concentrations for the same dental material (P < 0.01), but no significant difference existed between titanium-alloy (250 g/L) and negative control groups (P > 0.05). CONCLUSION: Co-Cr alloy, titanium alloy, and austenitic stainless steel have apparent influence on cellular apoptosis. The apoptosis rate is the greatest in the Co-Cr alloy group, followed by austenitic stainless steel group, and lastly titanium alloy group.