CAJ | 학술논문

在pH 6.3的NaH_2PO_4-Na_2HPO_4缓冲溶液中, 壳聚糖对荧光素的荧光强度具有明显的猝灭作用, 且在一定浓度范围内, 其猝灭程度与加入的壳聚糖浓度成线性关系, 据此建立了一种新的测定壳聚糖含量的荧光猝灭分光光度法. 该方法的回归方程为ΔF=64.02+42.28ρ (mg/L), R~2＝0.9942, 线性范围为0.50～10.0 mg/L, 检出限为0.27 mg/L. 样品测定的RSD为4.5% (n=6), 平均回收率为99.3%. 采用该方法可测定复杂样品中的壳聚糖含量.
재pH 6.3적NaH_2PO_4-Na_2HPO_4완충용액중, 각취당대형광소적형광강도구유명현적졸멸작용, 차재일정농도범위내, 기졸멸정도여가입적각취당농도성선성관계, 거차건립료일충신적측정각취당함량적형광졸멸분광광도법. 해방법적회귀방정위ΔF=64.02+42.28ρ (mg/L), R~2＝0.9942, 선성범위위0.50～10.0 mg/L, 검출한위0.27 mg/L. 양품측정적RSD위4.5% (n=6), 평균회수솔위99.3%. 채용해방법가측정복잡양품중적각취당함량.
A sensitive fluorescence quenching method was developed for the determination of chitosan based on its notable quenching effect on the fluorescence of fluorescein in a sodium phosphate buffer solution (pH 6.3). The quenched degree of fluorescence of fluorescein at a fixed concentration was in linear relationship with the added concentration of chitosan. The linear equation of the method was AF = 64.02 + 42.28,0 (mg/L) (R~2 =0.9942) with the linear range of 0.5～10.0 mg/L and the detection limit of 0.27 mg/L. The relative standard deviation was 4.5% (n=6) and the mean recovery was 99.3%. This method can be applied to the determination of chitosan in complex samples.