CAJ | 학술논문

目的千金藤属植物具有良好药用价值,许多研究表明其含有丰富的生物碱成分,但对该属植物其他成分的研究很少.对千金藤属植物非生物碱成分的组成和活性的研究,有利于更加深入地了解该属植物的药效和药理作用.方法从防己科千金藤属植物地不容Stephania epigeae H .S .Lo及云南地不容Stephania yunnanensis H.S.Lo中,采用苯酚硫酸测定法、铜试剂沉淀测定法分别测定地不容和云南地不容中的总多糖和葡聚糖的含量.结果地不容和云南地不容中分别含有25.14%,13.66%的总多糖及1.00%,0.71%的葡聚糖.结论苯酚硫酸法和铜试剂沉淀法联合使用,为该植物多糖的分离纯化及多糖成分的结构、活性研究提供可行手段.千金藤属植物的葡聚糖具有免疫促进作用,提示可对地不容的多糖成分做活性筛选研究.千金藤属植物的药用价值较高,目前对地不容的生物碱成分研究比较透彻,但对其非生物碱成分研究仍处于空白,此项研究将有助于推动其药用价值的开发.
목적 천금등속식물구유량호약용개치,허다연구표명기함유봉부적생물감성분,단대해속식물기타성분적연구흔소.대천금등속식물비생물감성분적조성화활성적연구,유리우경가심입지료해해속식물적약효화약리작용.방법 종방기과천금등속식물지불용Stephania epigeae H .S .Lo급운남지불용Stephania yunnanensis H.S.Lo중,채용분분류산측정법、동시제침정측정법분별측정지불용화운남지불용중적총다당화포취당적함량.결과 지불용화운남지불용중분별함유25.14%,13.66%적총다당급1.00%,0.71%적포취당.결론 분분류산법화동시제침정법연합사용,위해식물다당적분리순화급다당성분적결구、활성연구제공가행수단.천금등속식물적포취당구유면역촉진작용,제시가대지불용적다당성분주활성사선연구.천금등속식물적약용개치교고,목전대지불용적생물감성분연구비교투철,단대기비생물감성분연구잉처우공백,차항연구장유조우추동기약용개치적개발.
Objective Stephania is valuable in officinal using. It was indicated in many researches that Stephania contents is plenty of alkaloid. Other compositions have scarcely been studied. The study on non-alkaloid compositions would help us to comprehend its pharmic effects. Methods Extracted polysaccharides from Menispermaceae Stephania epigeae H .S .Lo and Stephania yunnanensis H.S.Lo. Determined the contents of total polysaccharides and glucans.Results The results of experiments indicated that there were 25.14% total polysaccharides in Stephania epigeae H .S .Lo and 13.66% in Stephania yunnanensis H.S.Lo., while 1.00% glucans in Stephania epigeae H .S .Lo and 0.71% in Stephania yunnanensis H.S.Lo..Conclusion An effective technique is provided to purify and separate the polysaccharides of Stephania in these experiments. Stephania is a kind of officinal herb. The study on alkaloid is sufficient, but it is void on the research of non-alkaloid compositions. This study is helpful to develop the officinal using of the Stephania.