光谱学与光谱分析
光譜學與光譜分析
광보학여광보분석
SPECTROSCOPY AND SPECTRAL ANALYSIS
2010年
3期
692-696
,共5页
高泽红%胡波%丁超%于晶宫
高澤紅%鬍波%丁超%于晶宮
고택홍%호파%정초%우정궁
显微拉曼光谱%不饱和度%脂类%结直肠组织%癌症
顯微拉曼光譜%不飽和度%脂類%結直腸組織%癌癥
현미랍만광보%불포화도%지류%결직장조직%암증
Micro-Raman spectroscopy%Unsaturation%Lipid%Colorectal tissue%Cancer
利用共聚焦显微拉曼光谱系统,检测了结直肠癌组织及邻近正常组织的拉曼光谱234例,从分子水平探讨结直肠癌变的原因.实验结果表明:在900~2 100 cm~(-1)频移范围内,与正常组织比较,归属于结直肠癌组织中脂类伸缩振动的1 067和1 133 cm~(-1)拉曼峰强度相对较弱,扭转振动的1 299 cm~(-1)与CH面内变形振动的1 264 cm~(-1)拉曼峰的强度比值减少,应用SPSS16.0统计学软件进行平均值计算与比较,正常组织中的1 299和1 264 cm~(-1)的强度比值平均值为3.17,癌变组织的为1.33;二者的分界值为1.96,以此判别结直肠癌组织和正常组织符合率为94%.研究表明,结直肠癌组织中脂类的链内纵向有序性下降,不饱和度增加.可推测结直肠癌组织中脂类的能量储存增加是引起这些变化的原因,而这些变化将导致细胞膜的流动性增强,从而改变细胞膜的物质运输、能量传递和信号传导,终将引起DNA结构的变化.因此,脂类的能量分布变化可能是导致细胞癌变的原因之一.
利用共聚焦顯微拉曼光譜繫統,檢測瞭結直腸癌組織及鄰近正常組織的拉曼光譜234例,從分子水平探討結直腸癌變的原因.實驗結果錶明:在900~2 100 cm~(-1)頻移範圍內,與正常組織比較,歸屬于結直腸癌組織中脂類伸縮振動的1 067和1 133 cm~(-1)拉曼峰彊度相對較弱,扭轉振動的1 299 cm~(-1)與CH麵內變形振動的1 264 cm~(-1)拉曼峰的彊度比值減少,應用SPSS16.0統計學軟件進行平均值計算與比較,正常組織中的1 299和1 264 cm~(-1)的彊度比值平均值為3.17,癌變組織的為1.33;二者的分界值為1.96,以此判彆結直腸癌組織和正常組織符閤率為94%.研究錶明,結直腸癌組織中脂類的鏈內縱嚮有序性下降,不飽和度增加.可推測結直腸癌組織中脂類的能量儲存增加是引起這些變化的原因,而這些變化將導緻細胞膜的流動性增彊,從而改變細胞膜的物質運輸、能量傳遞和信號傳導,終將引起DNA結構的變化.因此,脂類的能量分佈變化可能是導緻細胞癌變的原因之一.
이용공취초현미랍만광보계통,검측료결직장암조직급린근정상조직적랍만광보234례,종분자수평탐토결직장암변적원인.실험결과표명:재900~2 100 cm~(-1)빈이범위내,여정상조직비교,귀속우결직장암조직중지류신축진동적1 067화1 133 cm~(-1)랍만봉강도상대교약,뉴전진동적1 299 cm~(-1)여CH면내변형진동적1 264 cm~(-1)랍만봉적강도비치감소,응용SPSS16.0통계학연건진행평균치계산여비교,정상조직중적1 299화1 264 cm~(-1)적강도비치평균치위3.17,암변조직적위1.33;이자적분계치위1.96,이차판별결직장암조직화정상조직부합솔위94%.연구표명,결직장암조직중지류적련내종향유서성하강,불포화도증가.가추측결직장암조직중지류적능량저존증가시인기저사변화적원인,이저사변화장도치세포막적류동성증강,종이개변세포막적물질운수、능량전체화신호전도,종장인기DNA결구적변화.인차,지류적능량분포변화가능시도치세포암변적원인지일.
Confocal Raman microscopy system was used to investigate the etiology of colorectal cancer at the molecular level A total of two hundred and thirty four micro-Raman spectra were measured from thirty nine patients for surgically resected colorectal cancer specimens and adjacent normal tissues in the region 900-2 100 cm~(-1).Two bands at 1 064 and 1 134 cm~(-1) whicb are attributed to the all-trans bond stretching vibrations of the C-C lipid skeleton decreased in colorectal cancer tissue samples.The ratio of the Raman band intensity at 1 299 cm~(-1) assigned to in-phase CH_2 twisting vibrations of lipids to that at 1 264 cm~(-1) from CH in-plane deformation was different for colorectal cancerous and normal tissues.The mean values of the (I_(1 299)/I_(1 264)) were 3.17 for normal tissues and 1.33 for cancer tissues using the software function of "Compare Means".Further,the scatter plots were used for grouping the ratio of Raman intensity at 1 299 to 1 264 cm~(-1) according to tissue pathologic types by graph of the SPSS16.00 software.The decision lines (I_(1 299)/I_(1 264)=1.96) separates between cancer and normal tissue with a coincidence rate of 94% (188/200) according to the histological results by graph.The results show that the differences between cancer and normal tissue spectra appear to arise from a decrease in the degree of the longitudinal order and an increase in the degree of unsaturation of lipid,which causes the fluidity of lipid to increases when normal cells are transformed into cancerous cells.