CAJ | 학술논문

[目的]观察低氧对体外培养的人骨髓间充质干细胞(hBMSCs)向胆碱能神经元分化的影响.[方法]将对照组和常氧分化组hBMSCs置于常氧(20%O2环境中培养,低氧分化组细胞置于低氧(3%O2环境中.采用免疫细胞化学法和高效液相色谱-电化学法,分别检测胆碱能神经元的数量和诱导分化后乙酰胆碱(ACh)的含量.[结果]对照组未见胆碱乙酰化酶(CHAT)阳性神经元,低氧分化组ChAT阳性神经元数量明显增多,约为常氧分化组的4倍(P<0.01),并且该组诱导分化后的细胞合成的ACh含量也高于常氧分化组(P<0.05).[结论]低氧可促进hBMSCs向胆碱能神经元方向分化,这为临床应用hBMSCs治疗阿尔茨海默病提供了新的思路.
[목적]관찰저양대체외배양적인골수간충질간세포(hBMSCs)향담감능신경원분화적영향.[방법]장대조조화상양분화조hBMSCs치우상양(20%O2배경중배양,저양분화조세포치우저양(3%O2배경중.채용면역세포화학법화고효액상색보-전화학법,분별검측담감능신경원적수량화유도분화후을선담감(ACh)적함량.[결과]대조조미견담감을선화매(CHAT)양성신경원,저양분화조ChAT양성신경원수량명현증다,약위상양분화조적4배(P<0.01),병차해조유도분화후적세포합성적ACh함량야고우상양분화조(P<0.05).[결론]저양가촉진hBMSCs향담감능신경원방향분화,저위림상응용hBMSCs치료아이자해묵병제공료신적사로.
[Objective] To investigate the effect of hypoxia on the differentiation of human bone marrow mesenchymal stem cells (hBMSCs) into cholinergic neurons in vitro. [Methods] hBMSCs in the control group and the normoxic differentiation group were cultured under normoxic condition (20% O2, while cells in the hypoxic differentiation group were in hypoxic environment (3% O2. The number of differentiated cells was counted by immunocytochemical method and the content of acetylcholine (Ach) was measured by the electro-chemical detection of high-performance liquid chromatography (HPLC-ECD). [Results] Choline acetyltransferase (ChAT)-positive cells were not observed in the control group. ChAT-positive cells differentiated from the induced hBMSCs under hypoxic condition increased about 3 times as compared with that under normoxic condition (P<0.01). Furthermore, the content of Ach of the differentiated cells in hypoxia group was significantly higher than that of normoxic group (P<0.05). [Conclusions] Hypoxia improves the differentiation of hBMSCs into cholinergic neurons, which provides a new idea for clinical treatment of Alzheimer's disease with hBMSCs.