CAJ | 학술논문

管家基因GAPDH(glyceraldehyde-3-phosphate dehydrogenase)常被用作定量、半定量PCR试验的内参,以确定目标基因的相对表达量.基于前期从全长cDNA文库中获得GAPDH基因的EST单克隆,进行PrimerWalking测序,获得一个GAPDH的全长cDNA序列,序列长1 790 bp,编码421个蛋白氨基酸,命名为GoGAP-DH1.采用Blast,ExPASy,ProtParam,SPOMA,Find Conserved Domains(NCBI),ClustalX,MEGA等生物信息学软件对cDNA序列、氨基酸序列、保守区、亲缘关系等特征进行分析.结果表明,GoGAPDH1与玉米的细胞质GAPDH基因源性最高,为82%.初步推断GoGAPDH1可能是一个胞质型GAPDH.
관가기인GAPDH(glyceraldehyde-3-phosphate dehydrogenase)상피용작정량、반정량PCR시험적내삼,이학정목표기인적상대표체량.기우전기종전장cDNA문고중획득GAPDH기인적EST단극륭,진행PrimerWalking측서,획득일개GAPDH적전장cDNA서렬,서렬장1 790 bp,편마421개단백안기산,명명위GoGAP-DH1.채용Blast,ExPASy,ProtParam,SPOMA,Find Conserved Domains(NCBI),ClustalX,MEGA등생물신식학연건대cDNA서렬、안기산서렬、보수구、친연관계등특정진행분석.결과표명,GoGAPDH1여옥미적세포질GAPDH기인원성최고,위82%.초보추단GoGAPDH1가능시일개포질형GAPDH.