CAJ | 학술논문

目的探讨JAZF1过表达对鼠肝细胞IAR-20及Hepal-6糖脂代谢相关基因的影响.方法克隆鼠JAZF1编码区并构建pIRES2-EGFP-JAZF1表达载体,利用脂质体法将目的基因转染到鼠肝细胞IAR-20及Hepal-6中,采用实时荧光定量PCR测定各处理组JAZF1 mRNA表达情况,以及糖脂代谢相关基因SREBP1、AOC、FAS、HSL、PPARα、ATGL、GluT-1、GluT-4和细胞因子FGF-21mRNA的变化情况.结果 JAZF1基因在鼠肝细胞中过表达导致脂代谢基因SREBP1,AOC,FAS表达降低(P<0.01),PPARα和HSL表达增加(P<0.01),对ATGL无影响(P>0.05),使糖转运相关基因GluT-1表达增加(P<0.01),GluT-4无显著变化(P>0.05),对FGF-21无影响(P>0.05).结论 JAZF1过表达可以抑制肝细胞的脂肪生成,促进脂肪分解,并可能增加基础葡萄糖转运.
목적 탐토JAZF1과표체대서간세포IAR-20급Hepal-6당지대사상관기인적영향.방법 극륭서JAZF1편마구병구건pIRES2-EGFP-JAZF1표체재체,이용지질체법장목적 기인전염도서간세포IAR-20급Hepal-6중,채용실시형광정량PCR측정각처리조JAZF1 mRNA표체정황,이급당지대사상관기인SREBP1、AOC、FAS、HSL、PPARα、ATGL、GluT-1、GluT-4화세포인자FGF-21mRNA적변화정황.결과 JAZF1기인재서간세포중과표체도치지대사기인SREBP1,AOC,FAS표체강저(P<0.01),PPARα화HSL표체증가(P<0.01),대ATGL무영향(P>0.05),사당전운상관기인GluT-1표체증가(P<0.01),GluT-4무현저변화(P>0.05),대FGF-21무영향(P>0.05).결론 JAZF1과표체가이억제간세포적지방생성,촉진지방분해,병가능증가기출포도당전운.
Objective To investigate the role of JAZF1(juxtaposed with another zinc finger gene 1) in the regulation of glucose and lipid metabolism in hepatic cells. Methods The JAZF1 gene was transfected into hepatic IAR-20 cells and hepatoma carcinoma Hepa1-6 cells using vector-mediated pIRES2-EGFP-JAZF1. Expression of several genes which implicated in metabolism was measured using fluorescent quantitative PCR. Results In JAZF1-overexpressing hepatic cells, cellular SREBP1, ACC and FAS levels were down-regulated as compared with the control cells (P<0.01). The levels of PPARα and HSL mRNA were significantly increased (all P<0.01). But there was no significant difference in the expression of ATGL gene between JAZF1-overexpressing cells and control cells (P>0.05). In addition, the transcriptions of GluT-1 was significantly higher as compared with the control (P<0.001), but there was no statistical difference in the expression of GluT-4(P>0.05). Compared with the controls, JAZF1 had no effects on FGF-21 mRNA in cells. Conclusion Overexpression of JAZF1 promotes lipolysis, inhibits adipogenesis and increases basal glucose transport in hepatic cells.