CAJ | 학술논문

采用胶原酶消化法分离原代培养人网膜基质前脂肪细胞进行诱导分化.在诱导分化早期,前脂肪细胞因子1表达迅速降低,而脂蛋白脂酶、PPARγ2表达迅速增加,CAAT/增强子结合蛋白α(C/EBPα)在成熟阶段表达丰度最高.瘦素mRNA表达和分泌随分化逐渐增高,抵抗素mRNA仅在前脂肪细胞表达和分泌而脂联素mRNA仅在成熟脂肪细胞表达和分泌,胰岛素样生长因子I(IGF-I)在前脂肪细胞诱导分化前后均有分泌.
채용효원매소화법분리원대배양인망막기질전지방세포진행유도분화.재유도분화조기,전지방세포인자1표체신속강저,이지단백지매、PPARγ2표체신속증가,CAAT/증강자결합단백α(C/EBPα)재성숙계단표체봉도최고.수소mRNA표체화분비수분화축점증고,저항소mRNA부재전지방세포표체화분비이지련소mRNA부재성숙지방세포표체화분비,이도소양생장인자I(IGF-I)재전지방세포유도분화전후균유분비.
Stromal preadipocytes from human omental adipose tissues were isolated with enzyme-digesting method,and cultured for inducing differentiation.Preadipocyte factor-1 was abundant during preadipoeyte stage,whereas lipoprotein lipase and PPARγ2 mRNA were rapidly up-regulated at early stage of differentiation and CCAAT/enhancer binding protein expression was increased rather late.In addition,resistin expression and secretion was primarily detectable in preadipocytes,leptin was gradually increased during adipogenesis,whereas adiponectin appeared specifically in mature adipocytes.Insulin-like growth factor-Ⅰ secretion was remarkably detected in both preadipocytes and mature adipocytes.