中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2011年
11期
1019-1024
,共6页
准分子激光原位角膜磨镶术%眼压%基因芯片
準分子激光原位角膜磨鑲術%眼壓%基因芯片
준분자격광원위각막마양술%안압%기인심편
Laser in situ keratomileusis%Transient high intraocular pressure%Gene chip
目的 应用基因芯片技术研究负压吸引后兔视网膜的基因表达谱,探讨负压吸引引起RGCs凋亡可能的分子学机制.方法 12只健康新西兰大耳白兔随机分为实验对照组和负压吸引3min组.负压吸引3min组分别于术后即刻(0 d)、7d和10 d处死,取视网膜组织提取总RNA,利用Agilent公司兔单标芯片检测视网膜组织的基因表达谱,并分析差异表达基因.结果 负压吸引3min术后即刻差异表达基因共704条.上调基因485条,下调基因219条,其中凋亡相关基因共筛选出6条,上调5条,下调1条,上调表达较高的基因是CRYAA、CRYAB和TLR3,下调表达较高的基因是KRT18.负压吸引3min术后7d差异表达基因共482条.上调基因178条,下调基因304条,其中凋亡相关基因共筛选出4条,上调1条,下调3条,上调表达较高的基因是CRYAB,下调表达较高的基因是IL1 -BETA和IL1 R1.负压吸引3min术后10 d差异表达基因共402条.上调基因213条,下调基因189条,其中凋亡相关基因共筛选出6条,上调4条,下调2条,上调表达较高的基因是CRYAB、CRYBA3和CRYBB2,下调表达较高的基因是IL1 -BETA和IL1R1.在差异表达基因中除凋亡相关基因外还有能量代谢、物质转运、信号传导、发育、解毒抗氧化、免疫反应、细胞运动、细胞组织生物发生、分化和细胞黏附等相关基因.结论 负压吸引可使视网膜一些基因表达水平发生变化,其中基因差异倍数较高的基因多数对RG Cs凋亡起保护作用.
目的 應用基因芯片技術研究負壓吸引後兔視網膜的基因錶達譜,探討負壓吸引引起RGCs凋亡可能的分子學機製.方法 12隻健康新西蘭大耳白兔隨機分為實驗對照組和負壓吸引3min組.負壓吸引3min組分彆于術後即刻(0 d)、7d和10 d處死,取視網膜組織提取總RNA,利用Agilent公司兔單標芯片檢測視網膜組織的基因錶達譜,併分析差異錶達基因.結果 負壓吸引3min術後即刻差異錶達基因共704條.上調基因485條,下調基因219條,其中凋亡相關基因共篩選齣6條,上調5條,下調1條,上調錶達較高的基因是CRYAA、CRYAB和TLR3,下調錶達較高的基因是KRT18.負壓吸引3min術後7d差異錶達基因共482條.上調基因178條,下調基因304條,其中凋亡相關基因共篩選齣4條,上調1條,下調3條,上調錶達較高的基因是CRYAB,下調錶達較高的基因是IL1 -BETA和IL1 R1.負壓吸引3min術後10 d差異錶達基因共402條.上調基因213條,下調基因189條,其中凋亡相關基因共篩選齣6條,上調4條,下調2條,上調錶達較高的基因是CRYAB、CRYBA3和CRYBB2,下調錶達較高的基因是IL1 -BETA和IL1R1.在差異錶達基因中除凋亡相關基因外還有能量代謝、物質轉運、信號傳導、髮育、解毒抗氧化、免疫反應、細胞運動、細胞組織生物髮生、分化和細胞黏附等相關基因.結論 負壓吸引可使視網膜一些基因錶達水平髮生變化,其中基因差異倍數較高的基因多數對RG Cs凋亡起保護作用.
목적 응용기인심편기술연구부압흡인후토시망막적기인표체보,탐토부압흡인인기RGCs조망가능적분자학궤제.방법 12지건강신서란대이백토수궤분위실험대조조화부압흡인3min조.부압흡인3min조분별우술후즉각(0 d)、7d화10 d처사,취시망막조직제취총RNA,이용Agilent공사토단표심편검측시망막조직적기인표체보,병분석차이표체기인.결과 부압흡인3min술후즉각차이표체기인공704조.상조기인485조,하조기인219조,기중조망상관기인공사선출6조,상조5조,하조1조,상조표체교고적기인시CRYAA、CRYAB화TLR3,하조표체교고적기인시KRT18.부압흡인3min술후7d차이표체기인공482조.상조기인178조,하조기인304조,기중조망상관기인공사선출4조,상조1조,하조3조,상조표체교고적기인시CRYAB,하조표체교고적기인시IL1 -BETA화IL1 R1.부압흡인3min술후10 d차이표체기인공402조.상조기인213조,하조기인189조,기중조망상관기인공사선출6조,상조4조,하조2조,상조표체교고적기인시CRYAB、CRYBA3화CRYBB2,하조표체교고적기인시IL1 -BETA화IL1R1.재차이표체기인중제조망상관기인외환유능량대사、물질전운、신호전도、발육、해독항양화、면역반응、세포운동、세포조직생물발생、분화화세포점부등상관기인.결론 부압흡인가사시망막일사기인표체수평발생변화,기중기인차이배수교고적기인다수대RG Cs조망기보호작용.
Objective To detect the changes of gene expression profiles of retina by way of gene chip technology,and to investigate possible molecular pathogenesis of RGCs apoptosis caused by transient high IOP.Methods Twenty-two new Zealand white rabbits were randomized to experimental control and negative pressure suction 3 win groups.The rabbit were executed at different time points of post-LASIK,such as postoperative instant,7 d,10 d,then the total RNA of retinal tissue was extracted to detect gene expression profiles by the way of Agilent rabbit one-way gene chip.Results Instant after negative pressure suction,the genes differential expressed had 704 genes.Of these,the expression of 485 genes were unregulated and 219 genes down regulated.The higher expression of apoptosis genes were CRYAA,CRYAB,TLR3 and KRT18.7 d after negative pressure suction,the genes differential expressed had 482 genes.Of these,the expression of 178 genes were unregulated and 304 genes down regulated.The higher expression of apoptosis genes were CRYAB,IL1-BETA and IL1R1.10 d after negative pressure suction,the genes differential expressed had 402 genes.Of these,the expression of 213 genes were unregulated and 189 genes down regulated.The higher expression of apoptosis genes were CRYAB,CRYBA3,CRYBB2,IL1-BETA and IL1 R1.Conclusion The gene expression had changed after negative pressure suction.The genes with higher fold change protected RGCs from apoptosis.
