中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
2期
261-263
,共3页
邢细红%王雄伟%毛峰%郭东生%汪雷%吴胜梅
邢細紅%王雄偉%毛峰%郭東生%汪雷%吳勝梅
형세홍%왕웅위%모봉%곽동생%왕뢰%오성매
胶质瘤%表皮生长因子受体%增殖%细胞周期%脱噬作用
膠質瘤%錶皮生長因子受體%增殖%細胞週期%脫噬作用
효질류%표피생장인자수체%증식%세포주기%탈서작용
Glioma%Epidermal growth factor receptor%Proliferation%Cell cycle%Apoptosis
目的 观察表皮生长因子受体抑制剂Tyrphostin AG1478对人脑胶质瘤U87细胞增殖、细胞周期和细胞凋亡的影响.方法 噻唑蓝(MTT)比色法检测不同浓度Tyrphostin AG1478作用于体外培养的人脑胶质瘤U87细胞24 h后的细胞生存率,流式细胞仪检测不同浓度Tyrphostin AG1478作用于体外培养的人脑胶质瘤U87细胞24 h后细胞周期分布和细胞凋亡.结果 5、10、15、20 μmol/L的Tyrphostin AG1478作用人脑胶质瘤U87细胞24 h后细胞生存率分别为(7 8.93±11.95)%、(46.42±4.12)%、(42.13±7.54)%和(37.48±4.69)%;0、10、20 μmol/L的Tyrphostin AG1478作用人脑胶质瘤U87细胞24 h后细胞凋亡率分别为(10.19±3.15)%、(32.02±1.60)%和(54.35±2.80)%;0、10、20 μmol/L的Tyrphostin AG1478作用人脑胶质瘤U87细胞24 h后细胞主要分布在G0～G1期,分别为(51.20±1.21)%、(78.61±1.57)%和(82.73±0.77)%,实验组与对照组比较差异均有统计学意义(P＜0.05).结论 Tyrphostin AG1478抑制体外人脑胶质瘤细胞增殖,阻滞细胞周期在G0～G1期,诱导细胞凋亡,均呈浓度依赖性.
目的 觀察錶皮生長因子受體抑製劑Tyrphostin AG1478對人腦膠質瘤U87細胞增殖、細胞週期和細胞凋亡的影響.方法 噻唑藍(MTT)比色法檢測不同濃度Tyrphostin AG1478作用于體外培養的人腦膠質瘤U87細胞24 h後的細胞生存率,流式細胞儀檢測不同濃度Tyrphostin AG1478作用于體外培養的人腦膠質瘤U87細胞24 h後細胞週期分佈和細胞凋亡.結果 5、10、15、20 μmol/L的Tyrphostin AG1478作用人腦膠質瘤U87細胞24 h後細胞生存率分彆為(7 8.93±11.95)%、(46.42±4.12)%、(42.13±7.54)%和(37.48±4.69)%;0、10、20 μmol/L的Tyrphostin AG1478作用人腦膠質瘤U87細胞24 h後細胞凋亡率分彆為(10.19±3.15)%、(32.02±1.60)%和(54.35±2.80)%;0、10、20 μmol/L的Tyrphostin AG1478作用人腦膠質瘤U87細胞24 h後細胞主要分佈在G0～G1期,分彆為(51.20±1.21)%、(78.61±1.57)%和(82.73±0.77)%,實驗組與對照組比較差異均有統計學意義(P＜0.05).結論 Tyrphostin AG1478抑製體外人腦膠質瘤細胞增殖,阻滯細胞週期在G0～G1期,誘導細胞凋亡,均呈濃度依賴性.
목적 관찰표피생장인자수체억제제Tyrphostin AG1478대인뇌효질류U87세포증식、세포주기화세포조망적영향.방법 새서람(MTT)비색법검측불동농도Tyrphostin AG1478작용우체외배양적인뇌효질류U87세포24 h후적세포생존솔,류식세포의검측불동농도Tyrphostin AG1478작용우체외배양적인뇌효질류U87세포24 h후세포주기분포화세포조망.결과 5、10、15、20 μmol/L적Tyrphostin AG1478작용인뇌효질류U87세포24 h후세포생존솔분별위(7 8.93±11.95)%、(46.42±4.12)%、(42.13±7.54)%화(37.48±4.69)%;0、10、20 μmol/L적Tyrphostin AG1478작용인뇌효질류U87세포24 h후세포조망솔분별위(10.19±3.15)%、(32.02±1.60)%화(54.35±2.80)%;0、10、20 μmol/L적Tyrphostin AG1478작용인뇌효질류U87세포24 h후세포주요분포재G0～G1기,분별위(51.20±1.21)%、(78.61±1.57)%화(82.73±0.77)%,실험조여대조조비교차이균유통계학의의(P＜0.05).결론 Tyrphostin AG1478억제체외인뇌효질류세포증식,조체세포주기재G0～G1기,유도세포조망,균정농도의뢰성.
Objective To investigate the effects of Tyrphostin AG1478 on glioma U87 cells proliferation, cells cycle and apoptosis. Methods U87 cells were cultured for 24 h in the medium which contained AG1478 with different concentrations (0, 5, 10, 15, 20 μmol/L). The methyl thiazolyl tetrazolium(MTT) assay was used to detect the survival rate, and the cells cycle and apoptosis of the cells were examined by using flow cytometry. Results The cells proliferation was obviously inhibited by AG1478 in a dose-dependent manner. The survival rate of the cells in 5, 10, 15, 20 μmol/L AG1478 groups was (78.93 ±11.95)%, (46.42 ±4. 12)%, (42. 13 ±7.54)% and (37.48 ±4.69)% respectively, which was all significantly higher than that in 0 μ mol/L AG1478 group (P ＜0. 05 ). The apoptotic rate in 10, 25μmol/L AG1478 groups was (32.02 ± 1.60)% and (54. 35 ± 2. 80)% respectively, significantly higher than that in 0 μmol/L AG1478 group ( P ＜ 0. 05 ). The cells cycle was obviously inhibited by AG1478 in a dose-dependent manner. The percentage of cells treated with 10 and 20 μmol/L AG1478 in Go-G1 phase was ( 78. 61 ± 1.57 ) % and ( 82. 73 ± 0. 77 ) % respectively, significantly higher than that in 0 μmol/L AG1478 group (P ＜ 0. 05 ). Conclusion The growth inhibition of glioma U87 cells caused by AG1478 may be associated with apoptotsis induction and the G0-G1 arrest. AG1478 was expected to become a new anti-tumor drug in human glioma.
