中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2009年
24期
1-3
,共3页
CIK细胞%免疫表型%杀伤活性%流式细胞术%3H-TdR释放法
CIK細胞%免疫錶型%殺傷活性%流式細胞術%3H-TdR釋放法
CIK세포%면역표형%살상활성%류식세포술%3H-TdR석방법
CIK cell%Immune phenotypes%NK activities FACS%3H-TdR release assay
目的 观察自体CIK细胞治疗恶性肿瘤前后免疫表型和杀伤活性变化.方法 采用流式细胞术检测50例肿瘤患者CIK细胞治疗前、后免疫表型的变化,采用3H-TdR释放法检测杀伤活性的变化.结果 CIK治疗后,CD3+、CD4+细胞百分率上升,CD8+细胞百分率下调,CD4+/CD8+比值上升,CD19细胞百分率下降,与治疗前比较差异有统计学意义(P＜0.05),但CD3+、CD4+、CD8+细胞绝对值未达到健康者水平(P＞0.05).治疗后NK细胞百分率明显升高(P＜0.05),达到健康者水平(P＞0.05).在杀伤活性的研究中,与CIK细胞治疗前(19±6)%相比,治疗后杀伤活性百分率(22±5)%明显升高,达17.5%(P＜0.01),但未达到健康者水平(P＜0.05).结论 自体CIK细胞治疗能明显提高肿瘤患者细胞免疫功能.
目的 觀察自體CIK細胞治療噁性腫瘤前後免疫錶型和殺傷活性變化.方法 採用流式細胞術檢測50例腫瘤患者CIK細胞治療前、後免疫錶型的變化,採用3H-TdR釋放法檢測殺傷活性的變化.結果 CIK治療後,CD3+、CD4+細胞百分率上升,CD8+細胞百分率下調,CD4+/CD8+比值上升,CD19細胞百分率下降,與治療前比較差異有統計學意義(P＜0.05),但CD3+、CD4+、CD8+細胞絕對值未達到健康者水平(P＞0.05).治療後NK細胞百分率明顯升高(P＜0.05),達到健康者水平(P＞0.05).在殺傷活性的研究中,與CIK細胞治療前(19±6)%相比,治療後殺傷活性百分率(22±5)%明顯升高,達17.5%(P＜0.01),但未達到健康者水平(P＜0.05).結論 自體CIK細胞治療能明顯提高腫瘤患者細胞免疫功能.
목적 관찰자체CIK세포치료악성종류전후면역표형화살상활성변화.방법 채용류식세포술검측50례종류환자CIK세포치료전、후면역표형적변화,채용3H-TdR석방법검측살상활성적변화.결과 CIK치료후,CD3+、CD4+세포백분솔상승,CD8+세포백분솔하조,CD4+/CD8+비치상승,CD19세포백분솔하강,여치료전비교차이유통계학의의(P＜0.05),단CD3+、CD4+、CD8+세포절대치미체도건강자수평(P＞0.05).치료후NK세포백분솔명현승고(P＜0.05),체도건강자수평(P＞0.05).재살상활성적연구중,여CIK세포치료전(19±6)%상비,치료후살상활성백분솔(22±5)%명현승고,체17.5%(P＜0.01),단미체도건강자수평(P＜0.05).결론 자체CIK세포치료능명현제고종류환자세포면역공능.
Objective To investigate the difference of immune phenotypes and NK cell activity after treatment of autologous CIK cell on malignant tumor.Methods In the 50 patients with malignant tumor infused with CIK cell,the differences of immune phenotypes and activity of NK cells were respcetitively measured using FACS and 3H-TdR release assay.Results Compared with pre-treatment of autologous CIK cell,CD3+ and CD4+ expression percentage rise were up-regulated significantly,CD8+ cell expression percentage rise decline;The CD4+/CD8+ ratio rise,CD19 cell expression percentage rise decline,the comparison has significanct difference (P ＜ 0.05).After treatment of autologous CIK cell CD3-CD56+ cell expression percentage increase remarkablely.In the study of NK activity,NK activity (22 ±5)%,was higher obviously than pre-treatment(19± 6)%.Conclusions Treatment ofautologous C1K cell can enhance recent immunity function of the tumor patients obviously.
