中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2008年
10期
749-753
,共5页
毕晔%魏玲%毛海婷%张玲%左文述
畢曄%魏玲%毛海婷%張玲%左文述
필엽%위령%모해정%장령%좌문술
乳腺肿瘤%Fas%CTLA-4%RhoBTB2%RT-PCB
乳腺腫瘤%Fas%CTLA-4%RhoBTB2%RT-PCB
유선종류%Fas%CTLA-4%RhoBTB2%RT-PCB
Breast neoplasms%Fas%CTLA-4%RhoBTB2%RT-PCR
目的 探讨乳腺癌组织中Fas、CTLA-4和RhoBTW2基因表达的临床意义.方法 应用半定量逆转录聚合酶链反应(RT-PCR),检测60例原发乳腺癌患者癌组织和30例乳腺正常腺体组织中Fas、CTLA-4和RhoBTB2基因的mRNA表达,分析其与患者临床病理因素的关系.结果 乳腺癌组织中Fas、CTLA-4及RhoBTB2基因的相对表达量分别为0.699±0.285、1.045±0.302和0.625±0.160,而在乳腺正常腺体组织中分别为0.502±0.178、0.418±0.140和0.843±0.218,差异有统计学意义(P<0.01).腋淋巴结转移患者乳腺癌组织中Fas基因的表达(0.782±0.313)显著高于无转移患者(0.557±0.146,P<0.01),Ⅱ期患者乳腺癌组织中CTLA-4基因的表达(0.978±0.330)低于Ⅲ期患者(1.134±0.240,P<0.05),浸润性导管癌患者乳腺癌组织RhoBTB2基因的表达(0.597±0.157)低于浸润性小叶癌(0.717±0.145,P<0.05).Fas、CTLA-4和RhoBTB2的mRNA表达均与乳腺癌患者年龄、雌激素受体(ER)、孕激素受体(PR)、人表皮生长因子受体2(Her-2)状态和生存时间无关,且3者间亦无显著相关(P>0.05).结论 Fas、CTLA-4和RhoBTB2的mRNA表达均与乳腺癌发生、发展有关.Fas mRNA表达与腋淋巴结转移有关,CTLA-4 mRNA表达与乳腺癌分期有关,RhoBTB2 mRNA表达与乳腺癌病理类型有关.
目的 探討乳腺癌組織中Fas、CTLA-4和RhoBTW2基因錶達的臨床意義.方法 應用半定量逆轉錄聚閤酶鏈反應(RT-PCR),檢測60例原髮乳腺癌患者癌組織和30例乳腺正常腺體組織中Fas、CTLA-4和RhoBTB2基因的mRNA錶達,分析其與患者臨床病理因素的關繫.結果 乳腺癌組織中Fas、CTLA-4及RhoBTB2基因的相對錶達量分彆為0.699±0.285、1.045±0.302和0.625±0.160,而在乳腺正常腺體組織中分彆為0.502±0.178、0.418±0.140和0.843±0.218,差異有統計學意義(P<0.01).腋淋巴結轉移患者乳腺癌組織中Fas基因的錶達(0.782±0.313)顯著高于無轉移患者(0.557±0.146,P<0.01),Ⅱ期患者乳腺癌組織中CTLA-4基因的錶達(0.978±0.330)低于Ⅲ期患者(1.134±0.240,P<0.05),浸潤性導管癌患者乳腺癌組織RhoBTB2基因的錶達(0.597±0.157)低于浸潤性小葉癌(0.717±0.145,P<0.05).Fas、CTLA-4和RhoBTB2的mRNA錶達均與乳腺癌患者年齡、雌激素受體(ER)、孕激素受體(PR)、人錶皮生長因子受體2(Her-2)狀態和生存時間無關,且3者間亦無顯著相關(P>0.05).結論 Fas、CTLA-4和RhoBTB2的mRNA錶達均與乳腺癌髮生、髮展有關.Fas mRNA錶達與腋淋巴結轉移有關,CTLA-4 mRNA錶達與乳腺癌分期有關,RhoBTB2 mRNA錶達與乳腺癌病理類型有關.
목적 탐토유선암조직중Fas、CTLA-4화RhoBTW2기인표체적림상의의.방법 응용반정량역전록취합매련반응(RT-PCR),검측60례원발유선암환자암조직화30례유선정상선체조직중Fas、CTLA-4화RhoBTB2기인적mRNA표체,분석기여환자림상병리인소적관계.결과 유선암조직중Fas、CTLA-4급RhoBTB2기인적상대표체량분별위0.699±0.285、1.045±0.302화0.625±0.160,이재유선정상선체조직중분별위0.502±0.178、0.418±0.140화0.843±0.218,차이유통계학의의(P<0.01).액림파결전이환자유선암조직중Fas기인적표체(0.782±0.313)현저고우무전이환자(0.557±0.146,P<0.01),Ⅱ기환자유선암조직중CTLA-4기인적표체(0.978±0.330)저우Ⅲ기환자(1.134±0.240,P<0.05),침윤성도관암환자유선암조직RhoBTB2기인적표체(0.597±0.157)저우침윤성소협암(0.717±0.145,P<0.05).Fas、CTLA-4화RhoBTB2적mRNA표체균여유선암환자년령、자격소수체(ER)、잉격소수체(PR)、인표피생장인자수체2(Her-2)상태화생존시간무관,차3자간역무현저상관(P>0.05).결론 Fas、CTLA-4화RhoBTB2적mRNA표체균여유선암발생、발전유관.Fas mRNA표체여액림파결전이유관,CTLA-4 mRNA표체여유선암분기유관,RhoBTB2 mRNA표체여유선암병리류형유관.
Objective The purpose of this study was to explore the clinical significance of expression of Fas, CTLA-4 and RhoBTB2 genes in breast carcinoma. Methods Semi-quantitative reverse transcriptive polymerase chain reaction (RT-PCR) was used to analyze the mRNA expression levels of the three genes in tumor tissues from 60 patients with primary breast cancer and normal breast tissues of 30 cases. The relationship between gene expression and clinicopathological factors were analyzed and determined. Results The relative expression levels (gray scale ratio between target gene and internal reference gene) of Fas, CTLA-4 and RhoBTB2 genes in breast carcinoma tissues were 0.699±0.285, 1.045±0.302 and 0.625±0.160, respectively. In the normal breast tissues, they were 0.502±0.178, 0.418±0.140 and 0.843±0.218, respectively. There were statistically significant differences of the expression of those three genes between carcinoma tissues and normal breast tissues (P<0.01 ). The expression level of Fas in carcinoma tissues was significantly higher in lymph node matastasis positive patients (0.782±0.313) than that in node-negative patients (0.557±0.146,P<0.01 ). The expression level of CTLA-4 gone in carcinoma tissues was lower in Ⅱ stage patients (0.978±0.330) than that in Ⅲ stage patients (1.134 ±0.240,P<0.05). The expression level of RhoBTB2 gene was lower in invasive duetal carcinoma (0. 597±0.157) than that in invasive lobular carcinoma (0.717±0.145,P< 0.05). There were no correlations of expression of the three genes at mRNA level and age, ER, PR, HER2 status and survival time. Furthermore, no correlation was seen among the three genes expression (P>0.05). Conclusion The expression of all the three genes at mRNA level is involved in genesis and progression of breast cancer. There exist correlations between Fas expression and axillary lymph node mataatasis, CTLA-4 expression and disease stage, and RhoBTB2 expression and pathological type of breast cancer.
