CAJ | 학술논문

根据拟南芥基因组数据库提供的信息,首次以特异引物经PCR技术克隆到拟南芥硫肽激素-α的一个前体基因--AtPSK3,并对其进行了测序.序列分析表明,所获得的AtPSK3基因全长为505 bp,含有一个内含子和两个没有3'-或5'-非转译区的外显子,与数据库提供的序列比较,同源性为100%.
근거의남개기인조수거고제공적신식,수차이특이인물경PCR기술극륭도의남개류태격소-α적일개전체기인--AtPSK3,병대기진행료측서.서렬분석표명,소획득적AtPSK3기인전장위505 bp,함유일개내함자화량개몰유3'-혹5'-비전역구적외현자,여수거고제공적서렬비교,동원성위100%.
A Phytosulfokine-α gene(AtPSK3)was amplified from genomic DNA of Arabidopsis by PCR,based on the sequence information from Arabidopdis genome database, and its complete DNA sequence was analyzed. Results indicated that the AtPSK3 gene contained 505 base pairs,consisting of one large intron and two exons without 3'-or 5'-UTR sequence, the sequence of which has a homology of 100 percent as compared with the reported sequence.