CAJ | 학술논문

目的　探讨严重烧伤对大鼠肠三叶因子（ITF）及其mRNA表达的影响及意义。　方法　48只健康成年Wistar大鼠，制成30%TBSAⅢ度烧伤模型并随机分成正常对照组及伤后1，2，3，5，7 d共6组，每组8只。采用原位杂交、免疫组化等手段观察伤后ITF 及ITF mRNA表达的变化，并使用高效液相色谱仪对肠黏膜ITF含量进行定量检测。　结果　正常对照组大鼠小肠中ITF及ITF mRNA均有一定表达，它们主要分布于肠绒毛杯状细胞中。烧伤后肠黏膜组织结构受损，ITF mRNA表达明显减少，肠杯状细胞合成和分泌ITF的能力大幅下降（P<0.01），特别是ITF二聚体的含量远远低于伤前［(369.33±65.56) ng/g］，伤后7 d［(15.83±4.40) ng/g］仅为伤前的4%。　结论　严重烧伤后肠黏膜组织结构受损是ITF合成下降的主要原因，而ITF特别是ITF二聚体含量的大幅下降又可加重肠黏膜损伤，延缓肠黏膜修复。
목적　탐토엄중소상대대서장삼협인자（ITF）급기mRNA표체적영향급의의。　방법　48지건강성년Wistar대서，제성30%TBSAⅢ도소상모형병수궤분성정상대조조급상후1，2，3，5，7 d공6조，매조8지。채용원위잡교、면역조화등수단관찰상후ITF 급ITF mRNA표체적변화，병사용고효액상색보의대장점막ITF함량진행정량검측。　결과　정상대조조대서소장중ITF급ITF mRNA균유일정표체，타문주요분포우장융모배상세포중。소상후장점막조직결구수손，ITF mRNA표체명현감소，장배상세포합성화분비ITF적능력대폭하강（P<0.01），특별시ITF이취체적함량원원저우상전［(369.33±65.56) ng/g］，상후7 d［(15.83±4.40) ng/g］부위상전적4%。　결론　엄중소상후장점막조직결구수손시ITF합성하강적주요원인，이ITF특별시ITF이취체함량적대폭하강우가가중장점막손상，연완장점막수복。
Objective　To explore the changes of intestinal trefoil factor(ITF) as well as ITF mRNA expression in rat small intestine after severe burn injury . 　Methods　The distribution and the content of ITF in intestine were quantitatively determined with in situ hybridization (ISH), immunohistochemistry and high performance liquid chromatography (HPLC). 　Results　ITF and ITF mRNA were distributed in whole small intestine and most of them localized in goblet cell of intestinal villus. After burn injury, the structure of intestinal mucosa was severely damaged and ITF mRNA expression was lessened. Moreover, the ability of goblet cell synthesis and ITF secretion, especially ITF dimmer were significantly decreased, i.e., from (369.33±65.56) ng/g before burn injury to (15.83±4.40) ng/g 7 days after burn injury, only accounting for 4% of that before injury.　Conclusions　The post burn damage to intestinal structure is the main cause for the declination in ability of goblet cell synthesis and ITF secretion; and ITF drop especially ITF dimmer can enhance intestinal mucosa injury and delay intestinal mucosa repair.