CAJ | 학술논문

目的:观察携带人IL-10基因的慢病毒(LV-hIL-10)对激活的星形胶质细胞的干预作用.方法:确定脂多糖(LPS)诱导DI TNC1细胞的最佳浓度和时间,利用表达白介素-10(IL-10)的慢病毒感染DI TNC1细胞,使用RT-PCR和ELISA法检测LPS诱导下DI TNC1细胞促炎因子TNF-α,IL-1β的表达改变.结果:LPS激活的DI TNC1细胞中,TNF-α和IL-1β的表达增加.LV-hIL-10感染DI TNC1细胞后,IL-10表达明显上调,LV-hIL-10感染LPS诱导的DI TNC1细胞后,明显抑制TNF-α,IL-1β mRNA的表达及其蛋白分泌.结论:LPS能激活星形胶质细胞产生免疫细胞样作用分泌TNF-α,IL-1β,而LV-hIL-10能抑制其分泌作用.
목적:관찰휴대인IL-10기인적만병독(LV-hIL-10)대격활적성형효질세포적간예작용.방법:학정지다당(LPS)유도DI TNC1세포적최가농도화시간,이용표체백개소-10(IL-10)적만병독감염DI TNC1세포,사용RT-PCR화ELISA법검측LPS유도하DI TNC1세포촉염인자TNF-α,IL-1β적표체개변.결과:LPS격활적DI TNC1세포중,TNF-α화IL-1β적표체증가.LV-hIL-10감염DI TNC1세포후,IL-10표체명현상조,LV-hIL-10감염LPS유도적DI TNC1세포후,명현억제TNF-α,IL-1β mRNA적표체급기단백분비.결론:LPS능격활성형효질세포산생면역세포양작용분비TNF-α,IL-1β,이LV-hIL-10능억제기분비작용.
Objective To investigate the intervention effect of lentivirus expressing human IL-10 (LV-hIL-10) on activated astrocytes.Methods DI TNC1 cell line was treated with different concentrations of lipopolysaccharide (LPS) and time points.The expression of proinflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukinb-1β (IL-1β) was detected by RT-PCR and ELISA.Moreover,the effect on the expression of proinflammatory cytokines TNF-α and IL-1β was analyzed in DI TNC1 cell lines infected with and without LV-hIL-10.Results The expression of TNF-α and IL-1β was increased in DI TNC1 induced by LPS.The expression of IL-10 was upregulated in DI TNC1 infected with LV-hIL-10.TNF-α and IL-1β were inhibited by IL-10 overexpression in DI TNC1 actived by LPS.Conclusion DI TNC1 is activated by LPS and secretes proinflammatory cytokines TNF-α and IL-1β as immune-like cells,and these activation is inhibited by hIL-10 overexpression.