中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2012年
7期
689-694
,共6页
李世军%余春%王定明%唐青%陶晓燕%李浩%庄妍%周敬祝%王月%田克诚%唐光鹏
李世軍%餘春%王定明%唐青%陶曉燕%李浩%莊妍%週敬祝%王月%田剋誠%唐光鵬
리세군%여춘%왕정명%당청%도효연%리호%장연%주경축%왕월%전극성%당광붕
狂犬病病毒%糖蛋白基因%同源性%进化树%贵州省
狂犬病病毒%糖蛋白基因%同源性%進化樹%貴州省
광견병병독%당단백기인%동원성%진화수%귀주성
rabies virus%glycoprotein gene%homogeny%phylogenetic tree%Guizhou Province
目的 分析贵州省狂犬病病毒的糖蛋白基因(G基因)序列特征,为狂犬病的有效预防和控制提供科学依据.方法 以RT- PCR扩增贵州省近年来不同地区的人和犬狂犬病病毒阳性脑组织标本狂犬病病毒G基因序列,对扩增产物测序后采用生物信息学软件进行序列分析.结果 贵州省狂犬病毒阳性标本经RT-PCR扩增、测序与拼接后得到8份狂犬病病毒阳性样本的G基因序列.同源性分析显示.8份狂犬病病毒阳性样本的G基因序列在核苷酸及氩基酸水平上彼此的同源性分别为87.4％～99.9％和83.3.％～100％,与狂犬病毒基因1型病毒株G基因的核苷酸和氨基酸序列同源性最高(分别为86.5％～87.O％和83.3％～100％).进化树分析显示,贵州省狂犬病病毒G基因的亲缘进化关系较近,且与狂犬病毒基因1～7型中的基因1型代表毒株的亲缘进化关系最近；除GZ01和GZ09外,其余狂犬病毒G基因与国内湖北、湖南、安徽、广西、江苏和上海毒株相距较近.除GZ09与国外的马来西亚和泰国代表毒株亲缘进化关系最近外,其与毒株与印度尼西亚毒株亲缘进化关系最近.结论 本研究从狂犬病病毒G基因水平证明贵州省狂犬病毒株属于基因l型,揭示了我省贵州省狂犬病毒与国内外已报道毒株的亲缘进化关系,该研究结果将为贵州省狂犬病的有效预防和控制提供科学依据.
目的 分析貴州省狂犬病病毒的糖蛋白基因(G基因)序列特徵,為狂犬病的有效預防和控製提供科學依據.方法 以RT- PCR擴增貴州省近年來不同地區的人和犬狂犬病病毒暘性腦組織標本狂犬病病毒G基因序列,對擴增產物測序後採用生物信息學軟件進行序列分析.結果 貴州省狂犬病毒暘性標本經RT-PCR擴增、測序與拼接後得到8份狂犬病病毒暘性樣本的G基因序列.同源性分析顯示.8份狂犬病病毒暘性樣本的G基因序列在覈苷痠及氬基痠水平上彼此的同源性分彆為87.4％～99.9％和83.3.％～100％,與狂犬病毒基因1型病毒株G基因的覈苷痠和氨基痠序列同源性最高(分彆為86.5％～87.O％和83.3％～100％).進化樹分析顯示,貴州省狂犬病病毒G基因的親緣進化關繫較近,且與狂犬病毒基因1～7型中的基因1型代錶毒株的親緣進化關繫最近；除GZ01和GZ09外,其餘狂犬病毒G基因與國內湖北、湖南、安徽、廣西、江囌和上海毒株相距較近.除GZ09與國外的馬來西亞和泰國代錶毒株親緣進化關繫最近外,其與毒株與印度尼西亞毒株親緣進化關繫最近.結論 本研究從狂犬病病毒G基因水平證明貴州省狂犬病毒株屬于基因l型,揭示瞭我省貴州省狂犬病毒與國內外已報道毒株的親緣進化關繫,該研究結果將為貴州省狂犬病的有效預防和控製提供科學依據.
목적 분석귀주성광견병병독적당단백기인(G기인)서렬특정,위광견병적유효예방화공제제공과학의거.방법 이RT- PCR확증귀주성근년래불동지구적인화견광견병병독양성뇌조직표본광견병병독G기인서렬,대확증산물측서후채용생물신식학연건진행서렬분석.결과 귀주성광견병독양성표본경RT-PCR확증、측서여병접후득도8빈광견병병독양성양본적G기인서렬.동원성분석현시.8빈광견병병독양성양본적G기인서렬재핵감산급아기산수평상피차적동원성분별위87.4％～99.9％화83.3.％～100％,여광견병독기인1형병독주G기인적핵감산화안기산서렬동원성최고(분별위86.5％～87.O％화83.3％～100％).진화수분석현시,귀주성광견병병독G기인적친연진화관계교근,차여광견병독기인1～7형중적기인1형대표독주적친연진화관계최근；제GZ01화GZ09외,기여광견병독G기인여국내호북、호남、안휘、엄서、강소화상해독주상거교근.제GZ09여국외적마래서아화태국대표독주친연진화관계최근외,기여독주여인도니서아독주친연진화관계최근.결론 본연구종광견병병독G기인수평증명귀주성광견병독주속우기인l형,게시료아성귀주성광견병독여국내외이보도독주적친연진화관계,해연구결과장위귀주성광견병적유효예방화공제제공과학의거.
To study the characteristic of glycoprotein gene sequence of rabies virus in Guizhou Province in recent years and provide scientific basis for effective control and prevention of rabies,RT-PCR assay were used to detect human and dog brain tissues derived from different prefectures of Guizhou.The amplification products were sequenced and analyzed with bioinformatics software.The results showed that the full-length G gene sequences of 8 positive samples were obtained by RT-PCR amplification,sequencing and splicing.The homology of eight G gene sequences from Guizhou Province were between 87.4％ -99.9％ and 83.3.％- 100％ on nucleotide and deduced amino acid level,respectively,and the highest homology were found with the genotype 1 strains ( 86.5 ％- 87.0％ for nucleotide and 83.3 ％- 100 ％ for amino acid) among genotype 1- 7 representative strains.Besides,phylogenetic analysis based on the G gene indicated that the relationship of 8 strains derived from Guizhou were closest to genotype 1 Lyssavirus,and the strains of Guizhou were very close to the strains come from Hubei,Hunan,Anhui,Guangxi,Jiangsu provinces and Shanghai,except for GZ01 and GZ09.Moreover,GZ09 were evolutionarily closed to the strains come from Malaysia and Thailand,while the remaining sequences were closed to the strain of Indonesia.This study confirmed on the G gene level that rabies virus epidemic strains circulated in Guizhou Province in recent years belonged to rabies virus genotype 1 and the evolutionary relationship with reported strains come from different provinces of China and different countries were revealed in this study.It will provide scientific basis for effective control and prevention of human and animal rabies in Guizhou Province.
