CAJ | 학술논문

目的观察非肾七腺素能非碱胆能神经在冷刺激豚鼠食道诱导气道血浆渗出中的作用.方法豚鼠麻醉后,食管上下端插管,食管灌注4℃生理盐水.根据分组需要静脉注射阿托品及心得安阻断胆碱能及肾上腺素能通路,观察气管、主支气管、细支气管中微血管血浆渗出情况,以及SP受体拮抗剂FK888干预或预先切断迷走神经对微血管血浆渗出的影响.气道血浆渗出采用伊文思蓝法.结果冷刺激豚鼠食道显著增加气管、主支气管、细支气管的血浆渗出(P<0.01).SP受体拮抗剂FK888显著抑制冷刺激诱导的气管和主支气管血浆渗出(P<0.05).双侧迷走神经切除后,冷刺激豚鼠食道引起的气管、主支气管血浆渗出被显著抑制(P<0.05).结论冷刺激食道可以诱导气道血浆渗出,其机制可能与非肾上腺素能非胆碱能通路有关.
목적 관찰비신칠선소능비감담능신경재랭자격돈서식도유도기도혈장삼출중적작용.방법 돈서마취후,식관상하단삽관,식관관주4℃생리염수.근거분조수요정맥주사아탁품급심득안조단담감능급신상선소능통로,관찰기관、주지기관、세지기관중미혈관혈장삼출정황,이급SP수체길항제FK888간예혹예선절단미주신경대미혈관혈장삼출적영향.기도혈장삼출채용이문사람법.결과 랭자격돈서식도현저증가기관、주지기관、세지기관적혈장삼출(P<0.01).SP수체길항제FK888현저억제랭자격유도적기관화주지기관혈장삼출(P<0.05).쌍측미주신경절제후,랭자격돈서식도인기적기관、주지기관혈장삼출피현저억제(P<0.05).결론 랭자격식도가이유도기도혈장삼출,기궤제가능여비신상선소능비담감능통로유관.
Objective To observe the role of nonadrenergic noncholinergic nerve in airway microvascular leakage induced by cold sodium chloride intra-oesophageal instillation in guinea pigs. Methods Guinea pigs were anaesthetised with pentobarbital sodium, a catheter was placed in the midoesophagus and distal esophagus, infusion of 4℃ NS into the esophagus. Introvenous injection of atropine and propanolol to block the cholinergic and adrenergic nerves according to the groups. The microvascular leakage of trachea,main bronchus,bronchiole was examined in the presence and absence of bilateral vagus nerve or NK-1-receptor antagonist FK888. The airway plasma leakage was evaluated by measuring extravasated Evans blue dye. Results Infusion of 4℃ NS into the esophagus significantly increased the tracheal,mainstem bronchial and bronchiolar Evans blue dye concentration ( P<0.01). The tracheal and mainstem bronchial plasma extravasation induced by intra-oesophageal 4℃ NS instillation were significantly inhibited by bilateral vagotomy and FK888 ( P<0.05). Conclusions Cold stimulation in oesophagus can induce airway microvascular leakage, which may be mediated by nonadrenergic noncholinergic nerve.