国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2010年
2期
79-82,前插2
,共5页
徐新宇%翟文倩%王国林%田心
徐新宇%翟文倩%王國林%田心
서신우%적문천%왕국림%전심
丙泊酚麻醉程度%大鼠前额叶皮层%多通道局部场电位%相位同步模式
丙泊酚痳醉程度%大鼠前額葉皮層%多通道跼部場電位%相位同步模式
병박분마취정도%대서전액협피층%다통도국부장전위%상위동보모식
Prefrontal cortex of rats%Anesthetic degree of propofol%Local field potentials%Synchronization
目的 研究在清醒、丙泊酚麻醉过程中、翻正反射恢复后3种状态下,大鼠前额叶皮层多通道局部场电位(LFPs)的同步模式,作为界定麻醉程度的客观指标.方法 利用成年SD大鼠5只,在大鼠前额叶皮层植入16通道微电极阵列10 d后,应用Cerebus多通道信号采集分析系统,记录大鼠在清醒、丙泊酚麻醉过程中、翻正反射恢复后3种状态下前额叶皮层的16通道信号.丙泊酚麻醉按0.1 mg/(kg体重·min)的剂量,恒速静脉输注.对记录的原始信号进行预处理,获取LFPs,应用Hilbert变换计算LFPs相位系列.选择参考通道,分别计算3种状态下4s时间段内其它15通道LFPs相位与参考通道LFPs相位的相关动态值,计算窗口200 ms,窗口移动步长50 ms.结果 大鼠在清醒状态下LFPs相位的相关动态值比在丙泊酚麻醉状态下小(P<0.05);恢复翻正反射后的LFPs相位相关动态值比清醒状态大(P<0.05),比麻醉状态下小(P<0.05).结论 大鼠在清醒状态下,其前额叶皮层LFPs相位未呈现同步性;在丙泊酚麻醉状态下,呈现高度的同步性;在麻醉复苏期间,同步性在2者之间.多通道LFPs相位的同步程度可以界定不同的麻醉程度.
目的 研究在清醒、丙泊酚痳醉過程中、翻正反射恢複後3種狀態下,大鼠前額葉皮層多通道跼部場電位(LFPs)的同步模式,作為界定痳醉程度的客觀指標.方法 利用成年SD大鼠5隻,在大鼠前額葉皮層植入16通道微電極陣列10 d後,應用Cerebus多通道信號採集分析繫統,記錄大鼠在清醒、丙泊酚痳醉過程中、翻正反射恢複後3種狀態下前額葉皮層的16通道信號.丙泊酚痳醉按0.1 mg/(kg體重·min)的劑量,恆速靜脈輸註.對記錄的原始信號進行預處理,穫取LFPs,應用Hilbert變換計算LFPs相位繫列.選擇參攷通道,分彆計算3種狀態下4s時間段內其它15通道LFPs相位與參攷通道LFPs相位的相關動態值,計算窗口200 ms,窗口移動步長50 ms.結果 大鼠在清醒狀態下LFPs相位的相關動態值比在丙泊酚痳醉狀態下小(P<0.05);恢複翻正反射後的LFPs相位相關動態值比清醒狀態大(P<0.05),比痳醉狀態下小(P<0.05).結論 大鼠在清醒狀態下,其前額葉皮層LFPs相位未呈現同步性;在丙泊酚痳醉狀態下,呈現高度的同步性;在痳醉複囌期間,同步性在2者之間.多通道LFPs相位的同步程度可以界定不同的痳醉程度.
목적 연구재청성、병박분마취과정중、번정반사회복후3충상태하,대서전액협피층다통도국부장전위(LFPs)적동보모식,작위계정마취정도적객관지표.방법 이용성년SD대서5지,재대서전액협피층식입16통도미전겁진렬10 d후,응용Cerebus다통도신호채집분석계통,기록대서재청성、병박분마취과정중、번정반사회복후3충상태하전액협피층적16통도신호.병박분마취안0.1 mg/(kg체중·min)적제량,항속정맥수주.대기록적원시신호진행예처리,획취LFPs,응용Hilbert변환계산LFPs상위계렬.선택삼고통도,분별계산3충상태하4s시간단내기타15통도LFPs상위여삼고통도LFPs상위적상관동태치,계산창구200 ms,창구이동보장50 ms.결과 대서재청성상태하LFPs상위적상관동태치비재병박분마취상태하소(P<0.05);회복번정반사후적LFPs상위상관동태치비청성상태대(P<0.05),비마취상태하소(P<0.05).결론 대서재청성상태하,기전액협피층LFPs상위미정현동보성;재병박분마취상태하,정현고도적동보성;재마취복소기간,동보성재2자지간.다통도LFPs상위적동보정도가이계정불동적마취정도.
Objective To study the phase coherence mode of multi-channel local field potentials (LFPs) of rats cortex under waking, propofol anesthetized and recovery of righting reflex situations and to apply it to estimate the anesthetic degree. Methods Five male Sprague-Dawley rats were selected and 16-channel micro-electrode matrix were implanted in the prefrontal cortex of the rats. Ten days later, 16-channel electric signals were recorded from waking rats using Cerebus 128 Data Acquisition System. Then the rats were anesthetized with propofol intravenously through the tail vein, with the induction dose of propofol of 10 mg/kg and anesthesia maintenance dose of put and the rat regained consciousness, 16-channel electric signals from anaesthetic rats were recorded. After preprocessing, phase coherence of LFPs was calculated among the data from these three situations. Phase coherences were calculated in each window between each signal channel LFP and reference channel LFP with 200-ms multitaper window sliding from the beginning. Time-varying phase coherence dynamic distribution was available between each channel LFP and reference channel LFP with 25-ms overlapping. Results The results shows that the phase coherence average number in waking situation is less than the number in anesthesia (P<0.05); the phase coherence average number in recovery situation is less than that of anesthesia (P<0.05), it is larger than the number in anesthetic situation on the contrary (P<0.05). Conclusion The result indicates that the no synchronization of the LFPs during the waking-up time was found. High synchronization of the LFPs during the anesthetic period was found, while the degree of synchronization of the LFPs during the recovery time was between that of waking-up time period and anesthetic period. It can be applied to identify anesthetic degree of propofol.
