CAJ | 학술논문

目的比较酸性膨润土和有机膨润土的细胞毒性.方法用CCK-8试验、中性红(NRU)试验、乳酸脱氢酶(LDH)试验、凋亡试验和溶血试验来检测2种土的细胞毒性.溶血试验以人的红细胞为靶细胞,暴露剂量为0,0.3125、0.6250、1.2500、2.5000mg/ml,暴露10min.其余4个试验均以人B淋巴细胞系(HMy2.CIR)为靶细胞,暴露剂量为0、10、20、30、60、120、180 μg/ml,暴露4 h.结果所有剂量2种膨润土的溶血率均明显高于对照组,差异有统计学意义(P＜0.05).CCK-8试验结果表明,酸性土和有机土剂量分别≥30、20μg/ml时,细胞活性明显低于对照组,差异有统计学意义(P＜0.01);NRU试验和LDH试验出现类似结果,并均呈剂量-效应关系.180μg/ml酸性土组与120、180μg/ml有机土组的早期细胞凋亡率明显高于对照组,差异有统计学意义(P＜0.05).5个体外试验的结果均表明,有机土毒性明显高于酸性土.结论 2种膨润土均可诱发细胞凋亡、细胞膜损伤等细胞毒性.有机土细胞毒性明显高于酸性土.
목적 비교산성팽윤토화유궤팽윤토적세포독성.방법 용CCK-8시험、중성홍(NRU)시험、유산탈경매(LDH)시험、조망시험화용혈시험래검측2충토적세포독성.용혈시험이인적홍세포위파세포,폭로제량위0,0.3125、0.6250、1.2500、2.5000mg/ml,폭로10min.기여4개시험균이인B림파세포계(HMy2.CIR)위파세포,폭로제량위0、10、20、30、60、120、180 μg/ml,폭로4 h.결과 소유제량2충팽윤토적용혈솔균명현고우대조조,차이유통계학의의(P＜0.05).CCK-8시험결과표명,산성토화유궤토제량분별≥30、20μg/ml시,세포활성명현저우대조조,차이유통계학의의(P＜0.01);NRU시험화LDH시험출현유사결과,병균정제량-효응관계.180μg/ml산성토조여120、180μg/ml유궤토조적조기세포조망솔명현고우대조조,차이유통계학의의(P＜0.05).5개체외시험적결과균표명,유궤토독성명현고우산성토.결론 2충팽윤토균가유발세포조망、세포막손상등세포독성.유궤토세포독성명현고우산성토.
Objective To study comparatively the cytotoxicity induced by acid bentonite and organic bentonite.Methods The cytotoxicity of two kinds of bentonite was detected using CCK8 assay,neutral red uptake(NRU) assay,lactate dehydrogenase(LDH) leakage assay,apoptosis assay and hemolysis assay.In hemolysis assay human erythrocytes served as target cells and were exposed to the two kinds of bentonite at the doses of 0,0.3125,0.6250,1.2500 and 2.5000 mg/ml for ten min.In other four assays,human B lymphoblast cells (HMy2.CIR) served as target cells and were exposed to the two kinds of bentonite at the doses of 0,10,20,30,60,120 and 180 μg/ml for four h.Results In hemolysis assay,the hemolysis rates induced by two kinds of bentonite at all doses were significantly higher than that of control (P＜0.05);in CCK-8 assay,the cellular activities in acid bentonite group at the doses ≥30 μg/ml and in organic bentonite group at the doses ≥20μg/ml were significantly lower than that of control (P＜0.01);the similar results appeared in NRU assay and LDH assay,and the dose-effect relationship was observed in above 4 assays.In apeptosis assay,the early apoptosis cell rates in acid bentonite group at the dose of 180 μg/ml and in organic bentonite group at the doses of 120,180 μg/ml were significantly higher than that of control (P＜0.05).Moreover,the results of five in vitro assays indicated the eytotoxicity induced by organic bentonite was higher than that induced by acid bentonite.Conclusion Two kinds of bentonite could induce cytotoxicity,such as apoptosis and damage of cell membrane.The cytotoxicity of organic bentonite is higher than that of acid bentonite due to the different industrial treatment and characteristics of two kinds of bentonite particles.