中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2011年
5期
295-299
,共5页
肝炎,丙型%基因型,多态性%单核苷酸%白细胞介素-12亚单位p40%3'非翻译区
肝炎,丙型%基因型,多態性%單覈苷痠%白細胞介素-12亞單位p40%3'非翻譯區
간염,병형%기인형,다태성%단핵감산%백세포개소-12아단위p40%3'비번역구
Hepatitis C%Genotype%Polymorphism,single nucleotide%Interleukin-12 subunit p40%3'untranslated regions
目的 探讨丙型肝炎患者IL-12p40 3'非翻译区rs3212227位点基因多态性与HCV感染的关系.方法 应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)和DNA测序的方法榆测127例丙型肝炎患者IL-12 p40 3'非翻译区rs3212227位点的基因型,采用荧光定量PCR技术测定丙型肝炎患者血清HCV RNA水平.组间基因型及等位基因分布频率比较采用χ2检验.结果 丙型肝炎患者IL-12 p40 3'非翻译区rs3212227位点AA、AC、CC基因型分布频率分别为34.6%、40.9%和24.4%.等位基因A、C分布频率分别为55.1%、44.9%.丙型肝炎患者中HCVRNA≥2.0×106拷贝/mL组IL-12 p40 rs3212227位点C等位基因携带者分布频率明显高于HCVRNA<2.0×106拷贝/mL组(χ2=7.367,P=0.007).IFN无应答组IL-12 p40 rs3212227位点C等位基因分布频率明显高于IFN应答组(χ2=4.942,P=0.026).结论 IL-12p40 3'非翻译区rs3212227位点基因多态性与HCV感染具有相关性,携带C等位基因的个体可能更利于HCV复制,而不利于IFN治疗.
目的 探討丙型肝炎患者IL-12p40 3'非翻譯區rs3212227位點基因多態性與HCV感染的關繫.方法 應用聚閤酶鏈反應-限製性片段長度多態性分析(PCR-RFLP)和DNA測序的方法榆測127例丙型肝炎患者IL-12 p40 3'非翻譯區rs3212227位點的基因型,採用熒光定量PCR技術測定丙型肝炎患者血清HCV RNA水平.組間基因型及等位基因分佈頻率比較採用χ2檢驗.結果 丙型肝炎患者IL-12 p40 3'非翻譯區rs3212227位點AA、AC、CC基因型分佈頻率分彆為34.6%、40.9%和24.4%.等位基因A、C分佈頻率分彆為55.1%、44.9%.丙型肝炎患者中HCVRNA≥2.0×106拷貝/mL組IL-12 p40 rs3212227位點C等位基因攜帶者分佈頻率明顯高于HCVRNA<2.0×106拷貝/mL組(χ2=7.367,P=0.007).IFN無應答組IL-12 p40 rs3212227位點C等位基因分佈頻率明顯高于IFN應答組(χ2=4.942,P=0.026).結論 IL-12p40 3'非翻譯區rs3212227位點基因多態性與HCV感染具有相關性,攜帶C等位基因的箇體可能更利于HCV複製,而不利于IFN治療.
목적 탐토병형간염환자IL-12p40 3'비번역구rs3212227위점기인다태성여HCV감염적관계.방법 응용취합매련반응-한제성편단장도다태성분석(PCR-RFLP)화DNA측서적방법유측127례병형간염환자IL-12 p40 3'비번역구rs3212227위점적기인형,채용형광정량PCR기술측정병형간염환자혈청HCV RNA수평.조간기인형급등위기인분포빈솔비교채용χ2검험.결과 병형간염환자IL-12 p40 3'비번역구rs3212227위점AA、AC、CC기인형분포빈솔분별위34.6%、40.9%화24.4%.등위기인A、C분포빈솔분별위55.1%、44.9%.병형간염환자중HCVRNA≥2.0×106고패/mL조IL-12 p40 rs3212227위점C등위기인휴대자분포빈솔명현고우HCVRNA<2.0×106고패/mL조(χ2=7.367,P=0.007).IFN무응답조IL-12 p40 rs3212227위점C등위기인분포빈솔명현고우IFN응답조(χ2=4.942,P=0.026).결론 IL-12p40 3'비번역구rs3212227위점기인다태성여HCV감염구유상관성,휴대C등위기인적개체가능경리우HCV복제,이불리우IFN치료.
Objective To investigate the association between single nucleotide polymorphism (SNP) of interleukin-12 (IL-12) p40 3'untranslated region rs3212227 site and hepatitis C virus (HCV) infection. Methods Patients with hepatitis C (n=127) were genotyped and analyzed for the SNP of IL-12 p40 rs3212227 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing. The serum HCV RNA levels of patients with hepatitis C were detected using real time fluorescence quantitative-polymerase chain reaction (FQPCR). Inter-group comparisons of genotype and allele frequency were analyzed using chi-square test.Results In patients with hepatitis C, the frequencies of AA, AC and CC genotypes of IL-12 p40 rs3212227 site were 34. 6% ,40. 9% and 24. 4% , respectively,and the frequencies of allele A, C of IL12 p40 rs3212227 site were 55.1% and 44. 9%, respectively. The frequency of rs3212227 C allele in patients with HCV RNA ≥2. 0× 106 copy/mL was higher than that in patients with HCV RNA <2. 0 ×106 copy/mL (χ2 =7. 367, P = 0. 007). The frequency of rs3212227 C allele in responders to interferon (IFN) therapy was lower than that in patients with nonresponse to IFN therapy (χ2 =4. 942,P=0. 026). Conclusions The SNP of rs3212227 is correlated with HCV infection. The carriers with C allele may be susceptible to HCV infection, while resistant to IFN therapy.
