中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2012年
3期
141-145
,共5页
中枢神经系统感染%基质金属蛋白酶9%脑脊髓液%酶联免疫吸附测定
中樞神經繫統感染%基質金屬蛋白酶9%腦脊髓液%酶聯免疫吸附測定
중추신경계통감염%기질금속단백매9%뇌척수액%매련면역흡부측정
Central nervous system infections%Matrix metalloproteinase 9%Cerebrospinal fluid%Enzyme-linked immunosorbent assay
目的 分析不同病原导致的中枢神经系统感染患者脑脊液(CSF)中金属基质蛋白酶-9(MMP-9)的水平及意义.方法 应用ELISA法检测10例结核性脑膜炎患者急性期及缓解期、10例化脓性脑膜炎、10例真菌性脑膜炎、10例病毒性脑炎患者CSF中的MMP-9水平,并选择10例非肿瘤非感染性头痛患者的CSF作为对照,采用t检验比较各组间的差异,采用Spearman等级相关法分析MMP-9与CSF中细胞数、糖、氯化物、蛋白的相关性.结果 结核性脑膜炎组、化脓性脑膜炎组、真菌性脑膜炎组和病毒性脑炎组CSF中MMP-9水平分别为(569.46±162.42)、(182.79±99.06)、(54.69±19.93)和(18.52±10.31) ng/mL,高于对照组的(3.51±1.53) ng/mL,结核性脑膜炎组与以上其他感染组及对照组比较,差异均有统计学意义(t=2.925、3.041、3.237、3.454,P=0.0340、0.0270、0.0080、0.0001).结核性脑膜炎组急性期MMP 9水平为(569.46±162.42) ng/mL,明显高于缓解期的(294.30±89.06) ng/mL.结核性脑膜炎组的MMP-9水平与CSF中蛋白呈正相关(r=0.509,P=0.044),与糖(r=-0.451,P-0.008)和氯化物(r=-0.637,P=0.007)均呈负相关,与细胞数无相关性(r=0.308,P=0.246),而其他组则不存在相关性.结论 MMP-9水平在结核性脑膜炎、化脓性脑膜炎患者CSF中升高尤其明显,可作为中枢神经系统感染的标志物,动态监测其含量对病因的鉴别及判断治疗效果有一定意义.
目的 分析不同病原導緻的中樞神經繫統感染患者腦脊液(CSF)中金屬基質蛋白酶-9(MMP-9)的水平及意義.方法 應用ELISA法檢測10例結覈性腦膜炎患者急性期及緩解期、10例化膿性腦膜炎、10例真菌性腦膜炎、10例病毒性腦炎患者CSF中的MMP-9水平,併選擇10例非腫瘤非感染性頭痛患者的CSF作為對照,採用t檢驗比較各組間的差異,採用Spearman等級相關法分析MMP-9與CSF中細胞數、糖、氯化物、蛋白的相關性.結果 結覈性腦膜炎組、化膿性腦膜炎組、真菌性腦膜炎組和病毒性腦炎組CSF中MMP-9水平分彆為(569.46±162.42)、(182.79±99.06)、(54.69±19.93)和(18.52±10.31) ng/mL,高于對照組的(3.51±1.53) ng/mL,結覈性腦膜炎組與以上其他感染組及對照組比較,差異均有統計學意義(t=2.925、3.041、3.237、3.454,P=0.0340、0.0270、0.0080、0.0001).結覈性腦膜炎組急性期MMP 9水平為(569.46±162.42) ng/mL,明顯高于緩解期的(294.30±89.06) ng/mL.結覈性腦膜炎組的MMP-9水平與CSF中蛋白呈正相關(r=0.509,P=0.044),與糖(r=-0.451,P-0.008)和氯化物(r=-0.637,P=0.007)均呈負相關,與細胞數無相關性(r=0.308,P=0.246),而其他組則不存在相關性.結論 MMP-9水平在結覈性腦膜炎、化膿性腦膜炎患者CSF中升高尤其明顯,可作為中樞神經繫統感染的標誌物,動態鑑測其含量對病因的鑒彆及判斷治療效果有一定意義.
목적 분석불동병원도치적중추신경계통감염환자뇌척액(CSF)중금속기질단백매-9(MMP-9)적수평급의의.방법 응용ELISA법검측10례결핵성뇌막염환자급성기급완해기、10례화농성뇌막염、10례진균성뇌막염、10례병독성뇌염환자CSF중적MMP-9수평,병선택10례비종류비감염성두통환자적CSF작위대조,채용t검험비교각조간적차이,채용Spearman등급상관법분석MMP-9여CSF중세포수、당、록화물、단백적상관성.결과 결핵성뇌막염조、화농성뇌막염조、진균성뇌막염조화병독성뇌염조CSF중MMP-9수평분별위(569.46±162.42)、(182.79±99.06)、(54.69±19.93)화(18.52±10.31) ng/mL,고우대조조적(3.51±1.53) ng/mL,결핵성뇌막염조여이상기타감염조급대조조비교,차이균유통계학의의(t=2.925、3.041、3.237、3.454,P=0.0340、0.0270、0.0080、0.0001).결핵성뇌막염조급성기MMP 9수평위(569.46±162.42) ng/mL,명현고우완해기적(294.30±89.06) ng/mL.결핵성뇌막염조적MMP-9수평여CSF중단백정정상관(r=0.509,P=0.044),여당(r=-0.451,P-0.008)화록화물(r=-0.637,P=0.007)균정부상관,여세포수무상관성(r=0.308,P=0.246),이기타조칙불존재상관성.결론 MMP-9수평재결핵성뇌막염、화농성뇌막염환자CSF중승고우기명현,가작위중추신경계통감염적표지물,동태감측기함량대병인적감별급판단치료효과유일정의의.
Objective To analyze the levels of matrix metalloproteinase 9 (MMP-9 ) in cerebrospinal fluid (CSF) of patients with central nervous system (CNS) infection caused by different pathogens.Methods The levels of MMP 9 in CSF were detected by enzyme linked immunosorbent assay (ELISA) in 10 patients with tuberculous meningitis in both acute phase and recovery phase,10 purulent meningitis,10 cryptococeal meningitis,10 viral encephalitis and 10 controls.The differences among the groups were compared by t test. The correlations between MMP-9 levels and the cell count,glucose, chloride, and protein in CSF were analyzed by Spearman rank correlation.Results The CSF MMP-9 levels in groups of tuberculous meningitis, purulent meningitis,cryptoeoccal meningitis and viral encephalitis were (569.46±162.42),(182.79±99.06),(54.69±19.93) and (18.52±10.31) ng/mL,respectively,which were all significantly higher than that in controls (3.51± 1.53) ng/mL. There were significant differences between tuberculous meningitis group and other groups (t=2.925,3.041,3.237,3.454;P 0.0340,0.0270,0.0080 and0.0001,respectively). Moreover,in tuberculous meningitis group,the MMP-9 level in acute phase was (569.46±162.42) ng/mL,which was significantly higher than that in recovry phase (294.30+89.06) ng/mL.The CSF level of MMP-9 in tuberculous meningitis group was positively correlated with CSF protein (r=0.509,P=0.044),negative correlated with CSF glucose (r=-0.451,P=0.008) and chloride (r=-0.637,P=0.007),but no correlation with CSF cell count (r=0.308,P=0.246). And there were no correlations in other groups. Conclusions MMP-9 level in CSF increases significantly in patients with tuberculous meningitis and purulent meningitis,which can be used as a marker of CNS infection.Dynamic monitoring of thc CSF level of MMP-9 may be meaningful for the diagnosis and treatment.
