中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
10期
893-897
,共5页
陆军%叶松%邓云%胡忠昌%秦蕊
陸軍%葉鬆%鄧雲%鬍忠昌%秦蕊
륙군%협송%산운%호충창%진예
猫爪草%苦参%夏枯草%狼毒%细胞因子
貓爪草%苦參%夏枯草%狼毒%細胞因子
묘조초%고삼%하고초%랑독%세포인자
Radix Ranunculi Ternati%Radix Sophorae Flavescentis%Prunella vulgaris L.%Stellera chamaejasme L.%Cytokine
目的 探讨猫爪草、苦参、夏枯草及狼毒4种中药提取物对尘肺结核患者感染的耐多药结核分枝杆菌(multiple drugs resistant bacillus tuberculosis,MDR-TB)感染小鼠免疫功能的调节作用.方法 采用MDR-TB菌液灌胃制备小鼠MDR-TB感染模型,将60只健康雄性小鼠随机分为6组,每组10只.正常组标准饲料喂养,模型组灌服生理盐水,其他4组分别灌服4种中药提取物,采用ELISA法检测小鼠血清中与结核免疫密切相关的细胞因子IFN-γ、IL-4、IL-10和IL-12含量的变化,同时分离出外周血单个核细胞( PBMC),抽提全部RNA,采用RT-PCR法检测PBMC中IFN-γ、IL-4、IL-10、IL-12及颗粒裂解肽(GLS)的mRNA变化.结果 猫爪草、苦参、夏枯草及狼毒4组小鼠血清中的IFN-γ含量依次为(2.01 ±0.73) pg/ml、(1.92±0.56) pg/ml、(1.98±0.67) pg/ml和(1.94±0.59) pg/ml,IL-4为(6.01±1.46) pg/ml、(6.12±1.35) pg/ml、( 6.47± 1.46) pg/ml和(6.15±1.44)pg/ml,IL-10为(12.09±3.07) pg/ml、( 12.45±4.01) pg/ml、(12.13±3.43) pg/ml和(12.54±3.78)pg/ml,IL-12为(2.99±0.89) pg/ml、(2.75±0.84) pg/ml、(3.02±0.86) pg/ml和(2.89±0.75) pg/ml.与模型组比较,IFN-γ、IL-12含量明显升高,IL-4、IL-10含量明显下降,差异有统计学意义(P＜0.05),尤其以猫爪草提取物的调节作用最为显著；在mRNA表达水平上,4种中药提取物组小鼠PBMC中IFN-γ、IL-12、GLS表达明显升高,IL-4、IL-10 mRNA表达明显下降,差异有统计学意义(P＜O.05或P＜0.01).结论 4种中药提取物均可明显增强小鼠的细胞免疫功能,其对免疫的调节作用是在基因转录水平上发挥作用的,从而为临床应用治疗尘肺结核提供了理论依据.
目的 探討貓爪草、苦參、夏枯草及狼毒4種中藥提取物對塵肺結覈患者感染的耐多藥結覈分枝桿菌(multiple drugs resistant bacillus tuberculosis,MDR-TB)感染小鼠免疫功能的調節作用.方法 採用MDR-TB菌液灌胃製備小鼠MDR-TB感染模型,將60隻健康雄性小鼠隨機分為6組,每組10隻.正常組標準飼料餵養,模型組灌服生理鹽水,其他4組分彆灌服4種中藥提取物,採用ELISA法檢測小鼠血清中與結覈免疫密切相關的細胞因子IFN-γ、IL-4、IL-10和IL-12含量的變化,同時分離齣外週血單箇覈細胞( PBMC),抽提全部RNA,採用RT-PCR法檢測PBMC中IFN-γ、IL-4、IL-10、IL-12及顆粒裂解肽(GLS)的mRNA變化.結果 貓爪草、苦參、夏枯草及狼毒4組小鼠血清中的IFN-γ含量依次為(2.01 ±0.73) pg/ml、(1.92±0.56) pg/ml、(1.98±0.67) pg/ml和(1.94±0.59) pg/ml,IL-4為(6.01±1.46) pg/ml、(6.12±1.35) pg/ml、( 6.47± 1.46) pg/ml和(6.15±1.44)pg/ml,IL-10為(12.09±3.07) pg/ml、( 12.45±4.01) pg/ml、(12.13±3.43) pg/ml和(12.54±3.78)pg/ml,IL-12為(2.99±0.89) pg/ml、(2.75±0.84) pg/ml、(3.02±0.86) pg/ml和(2.89±0.75) pg/ml.與模型組比較,IFN-γ、IL-12含量明顯升高,IL-4、IL-10含量明顯下降,差異有統計學意義(P＜0.05),尤其以貓爪草提取物的調節作用最為顯著；在mRNA錶達水平上,4種中藥提取物組小鼠PBMC中IFN-γ、IL-12、GLS錶達明顯升高,IL-4、IL-10 mRNA錶達明顯下降,差異有統計學意義(P＜O.05或P＜0.01).結論 4種中藥提取物均可明顯增彊小鼠的細胞免疫功能,其對免疫的調節作用是在基因轉錄水平上髮揮作用的,從而為臨床應用治療塵肺結覈提供瞭理論依據.
목적 탐토묘조초、고삼、하고초급랑독4충중약제취물대진폐결핵환자감염적내다약결핵분지간균(multiple drugs resistant bacillus tuberculosis,MDR-TB)감염소서면역공능적조절작용.방법 채용MDR-TB균액관위제비소서MDR-TB감염모형,장60지건강웅성소서수궤분위6조,매조10지.정상조표준사료위양,모형조관복생리염수,기타4조분별관복4충중약제취물,채용ELISA법검측소서혈청중여결핵면역밀절상관적세포인자IFN-γ、IL-4、IL-10화IL-12함량적변화,동시분리출외주혈단개핵세포( PBMC),추제전부RNA,채용RT-PCR법검측PBMC중IFN-γ、IL-4、IL-10、IL-12급과립렬해태(GLS)적mRNA변화.결과 묘조초、고삼、하고초급랑독4조소서혈청중적IFN-γ함량의차위(2.01 ±0.73) pg/ml、(1.92±0.56) pg/ml、(1.98±0.67) pg/ml화(1.94±0.59) pg/ml,IL-4위(6.01±1.46) pg/ml、(6.12±1.35) pg/ml、( 6.47± 1.46) pg/ml화(6.15±1.44)pg/ml,IL-10위(12.09±3.07) pg/ml、( 12.45±4.01) pg/ml、(12.13±3.43) pg/ml화(12.54±3.78)pg/ml,IL-12위(2.99±0.89) pg/ml、(2.75±0.84) pg/ml、(3.02±0.86) pg/ml화(2.89±0.75) pg/ml.여모형조비교,IFN-γ、IL-12함량명현승고,IL-4、IL-10함량명현하강,차이유통계학의의(P＜0.05),우기이묘조초제취물적조절작용최위현저；재mRNA표체수평상,4충중약제취물조소서PBMC중IFN-γ、IL-12、GLS표체명현승고,IL-4、IL-10 mRNA표체명현하강,차이유통계학의의(P＜O.05혹P＜0.01).결론 4충중약제취물균가명현증강소서적세포면역공능,기대면역적조절작용시재기인전록수평상발휘작용적,종이위림상응용치료진폐결핵제공료이론의거.
Objective To investigate the regulative effect of the extract of Radix Ranunculi Ternati,Radix Sophorae Flavescenti,Prunella vulgaris L.and Stellera chamaejasme L.on cellular immunity induced by multiple drugs resistant bacillus tuberculosis(MDR-TB) from pneumoconiosis patients complicated with tuberculosis in rats.Methods MDR-TB model in rats was induced by MDR-TB.Normal control group were feed by standard feed,model group were irrigated by normal saline,and the other groups were respectively feed by the extract of Chinese herbal medicines.The content of IFN-γ,IL-4,IL-10 and IL-12 were examined by ELISA.RT-PCR was used to detect the mRNA levels of them.Results The content of IFN-γof the four extracts of Chinese herbal medicines were (2.01 ±0.73 ),( 1.92±0.56),( 1.98 ±0.67 ) and (1.94±0.59) pg/ml,IL-4 were (6.01±1.46),(6.12±1.35),(6.47±1.46) and (6.15±1.44) pg/ml,IL-10 were (12.09±3.07),( 12.45±4.01 ),( 12.13±3.43) and (12.54±3.78) pg/ml,IL-12 were (2.99±0.89),(2.75±0.84),(3.02±0.86) and (2.89±0.75) pg/ml.Compared to the model group,they resulted in significant in serum IFN-γ,IL-12,IL-4 and IL-10 (P＜0.05 or P＜0.01 ),the mRNA levels of them were significantly (P＜0.05 or P＜0.01 ).Conclusion The four extracts of Chinese herbal medicines can enhance the cellar immunological function in rats from up-regulation of the level of genetic transcription,accordingly provide the theory base of healing of pneumoconiosis patients complicated with tuberculosis with them.
