CAJ | 학술논문

报道细胞分裂素和硝酸铵对欧洲云杉不定芽伸长过程的影响.采用5年生的欧洲云杉1个无性系不定芽丛来研究玉米素和硝酸铵对不定芽生长的影响.试验3种诱导类型(玉米素浓度分别为6.84、13.68、20.52 μmol·L-1,激动素浓度0.69 μmol·L-1,培养4周),玉米素最高浓度处理诱导的伸长芽数量最多,在4个无生长调节剂继代培养过程中,加入了1个硝酸铵浓度逐渐增加的继代培养过程.经不含生长调节剂的低浓度的硝酸铵(2.5 mmol·L-1)培养基的继代培养后转入含有细胞分裂素的诱导培养基,下一个继代培养基补充的硝酸铵浓度分别为2.5、7.5、12.5、17.5 mmol·L-1,8周(2个继代培养)后转入含有硝酸铵浓度为2.5 mmol·L-1的培养基培养.硝酸铵浓度为7.5 mmol·L-1的处理可形成数量最多的不定芽,这对于采用不同浓度的玉米素进行诱导的处理结果是一致的,但随着硝酸铵的浓度增至12.5 mmol·L-1和17.5 mmol·L-1时则不定芽的数量减少.欧洲云杉的胚轴外植体经1次或多次诱导后,细胞分裂素(BA、激动素、玉米素、2iP)促进了不定芽的分化和发育,其中BA由于可诱导产生大量的不定芽而成为最有效的细胞分裂素.玉米素虽然诱导不定芽相对弱一些,却在BA多次诱导后可促进芽的伸长.这对获得具有形成不定芽能力并可扩繁的芽丛非常重要.
보도세포분렬소화초산안대구주운삼불정아신장과정적영향.채용5년생적구주운삼1개무성계불정아총래연구옥미소화초산안대불정아생장적영향.시험3충유도류형(옥미소농도분별위6.84、13.68、20.52 μmol·L-1,격동소농도0.69 μmol·L-1,배양4주),옥미소최고농도처리유도적신장아수량최다,재4개무생장조절제계대배양과정중,가입료1개초산안농도축점증가적계대배양과정.경불함생장조절제적저농도적초산안(2.5 mmol·L-1)배양기적계대배양후전입함유세포분렬소적유도배양기,하일개계대배양기보충적초산안농도분별위2.5、7.5、12.5、17.5 mmol·L-1,8주(2개계대배양)후전입함유초산안농도위2.5 mmol·L-1적배양기배양.초산안농도위7.5 mmol·L-1적처리가형성수량최다적불정아,저대우채용불동농도적옥미소진행유도적처리결과시일치적,단수착초산안적농도증지12.5 mmol·L-1화17.5 mmol·L-1시칙불정아적수량감소.구주운삼적배축외식체경1차혹다차유도후,세포분렬소(BA、격동소、옥미소、2iP)촉진료불정아적분화화발육,기중BA유우가유도산생대량적불정아이성위최유효적세포분렬소.옥미소수연유도불정아상대약일사,각재BA다차유도후가촉진아적신장.저대획득구유형성불정아능력병가확번적아총비상중요.
The capability of different cytokinins (BA, kinetin, zeatin, 2iP) to stimulate the induction and development of adventitious buds was determined after single and repeated induction steps with hypocotyl explants. BA was the most effective cytokinin as far as it concerned the number of induced adventitious buds. Zeatin, although less effective for this pattern,promoted the elongation of buds even after repeated induction cycles contrary to BA. This is important to get propagating bud clusters with the ability to form elongating shoots. A five-year-old propagating clone line of adventitious bud clusters was used to study the influence of zeatin and ammonium nitrate on the shoot elongation. Three induction variants (6.84, 13.68,20.52 μmol· L-1 zeatin, each with 0.69 μmol· L-1 kinetin for 4 weeks) were tested. The highest zeatin concentration induced the largest number of elongating buds. The influence of one subculture step with an enhanced ammonium nitrate concentration during the growth regulator free shoot development period (4 subcultures) was tested. A subculture on a medium free of growth regulators with a low ammonium nitrate concentration (2.5 mmol· L-1) followed the cytokinin containing induction medium. The next subculture medium was supplemented with 2.5, 7.5, 12.5 and 17.5 mmol· L-1 ammonium nitrate. An 8-week period (2subcultures) followed with 2.5 mmol· L-1 ammonium nitrate. The treatment with 7.5 mmol· L-1 ammonium nitrate formed the highest number of elongating buds. This was identical for all zeatin concentrations used for induction. Increased ammonium nitrate concentrations during this treatment (12.5 mmol· L-1 and 17.5 mmol·L-1) decreased the number of elongating buds.