分析化学
分析化學
분석화학
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2009年
7期
1033-1036
,共4页
莫志宏%杨琳玲%杨小超%陈自锋
莫誌宏%楊琳玲%楊小超%陳自鋒
막지굉%양림령%양소초%진자봉
汞离子%寡核苷酸%纳米金%石英晶体微天平
汞離子%寡覈苷痠%納米金%石英晶體微天平
홍리자%과핵감산%납미금%석영정체미천평
Mercury ion%oligonucleotide%gold nanoparticle%quartz crystal microbalance
利用Hg2+与DNA中胸腺嘧啶(T)结合的高度特异性和纳米金在石英晶体微天平(QCM)上的信号放大作用,设计了一种简便灵敏的Hg2+检测方法.纳米金采用柠檬酸钠还原法制备,其表面用末端带巯基的寡核苷酸探针进行自组装修饰,并用6-巯基己-1-醇(MCH)部分取代表面探针,以减少杂交空间位阻.结果表明,寡核苷酸链长为9bp、T个数为7的序列具有较高灵敏度;线性范围为5.0~100 nmol/L;检出限为2.0 nmol/L;Ca2+、Mg2+等其它金属离子无明显干扰.用于环境水样中Hg2+的测定, RSD<2.9%;加标回收率为97.3%~101.2%
利用Hg2+與DNA中胸腺嘧啶(T)結閤的高度特異性和納米金在石英晶體微天平(QCM)上的信號放大作用,設計瞭一種簡便靈敏的Hg2+檢測方法.納米金採用檸檬痠鈉還原法製備,其錶麵用末耑帶巰基的寡覈苷痠探針進行自組裝脩飾,併用6-巰基己-1-醇(MCH)部分取代錶麵探針,以減少雜交空間位阻.結果錶明,寡覈苷痠鏈長為9bp、T箇數為7的序列具有較高靈敏度;線性範圍為5.0~100 nmol/L;檢齣限為2.0 nmol/L;Ca2+、Mg2+等其它金屬離子無明顯榦擾.用于環境水樣中Hg2+的測定, RSD<2.9%;加標迴收率為97.3%~101.2%
이용Hg2+여DNA중흉선밀정(T)결합적고도특이성화납미금재석영정체미천평(QCM)상적신호방대작용,설계료일충간편령민적Hg2+검측방법.납미금채용저몽산납환원법제비,기표면용말단대구기적과핵감산탐침진행자조장수식,병용6-구기기-1-순(MCH)부분취대표면탐침,이감소잡교공간위조.결과표명,과핵감산련장위9bp、T개수위7적서렬구유교고령민도;선성범위위5.0~100 nmol/L;검출한위2.0 nmol/L;Ca2+、Mg2+등기타금속리자무명현간우.용우배경수양중Hg2+적측정, RSD<2.9%;가표회수솔위97.3%~101.2%
A simple and sensitive method was developed for the detection of mercury ions with quartz crystal microbalance(QCM), based on the specific thymine(T)-Hg2+-T structure and gold nanoparticles. The gold nanoparticles were prepared by the citrate reduction method. In a self-assembled way, the particle surface was modified with the probe oligonucleotides which were partially replaced by 6-Mercaptohexan-1-ol to reduce the steric hindrance of hybridization. The sensitivity was optimized by the oligonucleotide with a strand length of 9bp and a T number of 7. The linear range was 5.0-100 nmol/L with a detection limit of 2.0 nmol/L. And Ca2+, Mg2+ and other metal ions had no significant interferences. This method was successfully applied for the detection of Hg2+in environmental water samples, with RSD less than 2.9% and recoveries of 97.3%-101.2%.