CAJ | 학술논문

目的探讨血管紧张素Ⅱ(AngⅡ)在体外培养的人眼小梁细胞(TMC)中的表达情况.方法原代和传代培养人眼TMC株,收集第3代人眼TMC用于制作细胞爬片.利用免疫组织化学染色法和Western blot法检测体外培养人眼TMC中AngⅡ蛋白的表达.结果传代培养的人眼TMC呈典型梭形,免疫组织化学检测显示体外培养的人眼TMC细胞膜和细胞质中可见AngⅡ的阳性颗粒,阴形对照片中未见有AngⅡ蛋白表达.Western blot法在相对分子质量为64 000处可见一明显蛋白条带,为AngⅡ蛋白的阳性表达.结论 AngⅡ在体外培养的人眼TMC中呈阳性表达,提示AngⅡ可能参与青光眼眼压和房水循环的调节.
목적 탐토혈관긴장소Ⅱ(AngⅡ)재체외배양적인안소량세포(TMC)중적표체정황.방법 원대화전대배양인안TMC주,수집제3대인안TMC용우제작세포파편.이용면역조직화학염색법화Western blot법검측체외배양인안TMC중AngⅡ단백적표체.결과 전대배양적인안TMC정전형사형,면역조직화학검측현시체외배양적인안TMC세포막화세포질중가견AngⅡ적양성과립,음형대조편중미견유AngⅡ단백표체.Western blot법재상대분자질량위64 000처가견일명현단백조대,위AngⅡ단백적양성표체.결론 AngⅡ재체외배양적인안TMC중정양성표체,제시AngⅡ가능삼여청광안안압화방수순배적조절.
Objective Research showed that angiotensin converting enzyme inhibitor and angiotensinⅡ(AngⅡ)receptor antagonist has good role of lowing-intraocular pressure.This study was to explore whether cultured human trabecular meshwork cells express AngⅡin vitro.MethodsThe human trabecular meshwork cells strains were cultured in DMEM+F12 medium containing 25% fetal bovine serum in vitro and passaged at the climbing sheet was prepared.The expression of AngⅡ in human trabecular meshwork cells was examined by immunohistochemistry,and AngⅡ protein was localized by Western blot.ResultsSubcultured cells showed spindle shape.AngⅡwas positively expressed in human trabecular meshwork cells by immunochemistry,showing the yellow-brown granule in cellular membrane and cytoplasm.A absence of response for AngⅡwas found in negative control sample.The band of AngⅡ protein was found at the relative molecular weight of 64 000 by Western blot.ConclusionThe result implies that human trabecular meshwork cells have the ability of synthesizing AngⅡ.It suggests that AngⅡ participates in the regulation of intraocular tension in glaucomous eye.