CAJ | 학술논문

目的通过对食管癌组织及癌旁组织中早期生长反应基因1(Egr-1)表达的研究,探讨Egr-1mRNA与食管癌患者预后的关系.方法应用RT-PCR技术检测68例食管癌患者Egr-1mRNA表达情况,并对68例患者进行生存期随访,分析其与患者预后的关系.结果 Egr-1mRNA在正常食管组织表达最高为1.201±0.347,在癌旁组织表达次之,为0.945±0.336,在癌灶中心组织表达最低,为0.567±0.404(F=12.709,P＜0.01,P＜0.05);68例癌组织和癌旁组织标本中Egr-1mRNA均阳性表达的21例,单一表达阳性的21例,均阴性表达的26例.随访68例术后患者,术后1年生存组60例、术后3年生存组35例;2组Egr-1mRNA均阳性表达率分别为30.00%和65.71%(P＜0.01);2组阳性表达者生存率分别是94.44%和86.96%(P＞0.05).结论从癌旁组织到癌组织Egr-1mRNA表达减弱可能与食管癌的癌变有一定关系;食管癌组织Egr-1mRNA表达水平与患者生存期有关系,对食管癌预后判断有重要参考价值.
목적 통과대식관암조직급암방조직중조기생장반응기인1(Egr-1)표체적연구,탐토Egr-1mRNA여식관암환자예후적관계.방법 응용RT-PCR기술검측68례식관암환자Egr-1mRNA표체정황,병대68례환자진행생존기수방,분석기여환자예후적관계.결과 Egr-1mRNA재정상식관조직표체최고위1.201±0.347,재암방조직표체차지,위0.945±0.336,재암조중심조직표체최저,위0.567±0.404(F=12.709,P＜0.01,P＜0.05);68례암조직화암방조직표본중Egr-1mRNA균양성표체적21례,단일표체양성적21례,균음성표체적26례.수방68례술후환자,술후1년생존조60례、술후3년생존조35례;2조Egr-1mRNA균양성표체솔분별위30.00%화65.71%(P＜0.01);2조양성표체자생존솔분별시94.44%화86.96%(P＞0.05).결론 종암방조직도암조직Egr-1mRNA표체감약가능여식관암적암변유일정관계;식관암조직Egr-1mRNA표체수평여환자생존기유관계,대식관암예후판단유중요삼고개치.
Objective To study the expression of early growth response gene-1(Egr-1)in esophageal carcinoma tissue,and to explore the relationship between Egr-1 and the survival time of the patients with esophageal carcinoma.Methods RT-PCR was performed to detect the expression of Egr-1 mRNA in68 cases of esophageal carcinoma.The relationship between survival time and prognosis was analyzed.Results The expression of Egr-1 mRNA was the lowest(0.567±0.404),(0.945±0.336)and(1.201±0.347)in esophageal carcinoma tissue,para-cancerous tissue and normal esophageal mucosa tissue,respectively(F=12.709,P＜0.05,P＜0.00).21 cases showed the positive expression of Egr-1 mRNA of both the esophageal carcinoma tissue and the para-cancerous tissue.21 cases showed the positive expression of Egr-1 mRNA in a single esophageal carcinoma tissue or the para-cancerous tissue.26 cases showed the negative expression of Egr-1 mRNA both in the esophageal carcinoma tissue and the paracancerous tissue.The positive rate of Egr-1mRNA expression was 65.71%and 30.00%in the groups of the survival time for three years and the groups of the survival time for one year(P＜0.05).The survival rates in the two groups with positive expression of Egr-1 mRNA were 94.44%and 86.96%,respectively(P＞0.05).Conclusion Decreased level of EGR1 expression may be related to esophageal carcinogenesis.The expression level of Egr-1 mRNA might be associated with the survival time of the patients with esophageal carcinoma.Egr-1 expression in esophageal carcinoma tissue may be of great value for determining prognosis of esophageal carcinoma.