中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2012年
2期
46-49
,共4页
罗敏%林万飞%余倩莹%陈磊平
囉敏%林萬飛%餘倩瑩%陳磊平
라민%림만비%여천형%진뢰평
慢性荨麻疹%实时定量PCR%T-bet%GATA-3
慢性蕁痳疹%實時定量PCR%T-bet%GATA-3
만성담마진%실시정량PCR%T-bet%GATA-3
Chronic urticaria%Real-time quantitative PCR%T-bet%GATA-3
目的 检测慢性荨麻疹患者外周血单-核细胞(PBMC)T-bet、GATA-3 mRNA的表达,计算T-bet/GATA-3比值,探讨Th1/Th2失衡与慢性荨麻疹的关系.方法 采用实时定量多聚合酶链反应(RT-PCR)方法检测30例慢性荨麻疹患者(CU)和30例健康对照者PBMC中T-bet和GATA-3 mRNA的表达水平,并计算T-bet/GATA-3比值.结果 CU组PBMC中T-bet mRNA表达水平较对照组降低,而GATA-3 mRNA的水平较对照组升高,CU组T-bet/GATA-3比值较对照组显著下降.结论 慢性荨麻疹患者T-bet/GATA-3表达异常,患者存在显著的Th1/Th2免疫失衡.
目的 檢測慢性蕁痳疹患者外週血單-覈細胞(PBMC)T-bet、GATA-3 mRNA的錶達,計算T-bet/GATA-3比值,探討Th1/Th2失衡與慢性蕁痳疹的關繫.方法 採用實時定量多聚閤酶鏈反應(RT-PCR)方法檢測30例慢性蕁痳疹患者(CU)和30例健康對照者PBMC中T-bet和GATA-3 mRNA的錶達水平,併計算T-bet/GATA-3比值.結果 CU組PBMC中T-bet mRNA錶達水平較對照組降低,而GATA-3 mRNA的水平較對照組升高,CU組T-bet/GATA-3比值較對照組顯著下降.結論 慢性蕁痳疹患者T-bet/GATA-3錶達異常,患者存在顯著的Th1/Th2免疫失衡.
목적 검측만성담마진환자외주혈단-핵세포(PBMC)T-bet、GATA-3 mRNA적표체,계산T-bet/GATA-3비치,탐토Th1/Th2실형여만성담마진적관계.방법 채용실시정량다취합매련반응(RT-PCR)방법검측30례만성담마진환자(CU)화30례건강대조자PBMC중T-bet화GATA-3 mRNA적표체수평,병계산T-bet/GATA-3비치.결과 CU조PBMC중T-bet mRNA표체수평교대조조강저,이GATA-3 mRNA적수평교대조조승고,CU조T-bet/GATA-3비치교대조조현저하강.결론 만성담마진환자T-bet/GATA-3표체이상,환자존재현저적Th1/Th2면역실형.
Objective To detect the T-bet and GATA-3 expression in chronic urticaria patients,and calculate the ratio of T-bet/GATA-3,then to investigate the relationship between the immune-imbalance and chronic urticaria.Methods Real-time quantitative PCR technology was used to detect the expression of T-bet and GATA-3 in peripheral blood mononuclear ceils from 30 chronic urticaria patients(CU group)and 30 normal in dividuals(control group).And then the ratio of T-bet / GATA-3 was calculated and compared.Results Tbet expression in CU group was lower than control group(P =0.0001),while GATA-3 was higher than that in control group(P =0.0001),and the ratio of T-bet / GATA-3 in CU group was obviously lower than that in control group (P =0.0001).Conclusions T-bet / GATA-3 decreased in chronic urticaria patients,there is prominent immune imbalance of Th1/Th2 in the chronic urticaria.
