中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2008年
11期
821-824
,共4页
肾肿瘤%免疫组织化学%Western blot%RT-PCR%ZNRD1
腎腫瘤%免疫組織化學%Western blot%RT-PCR%ZNRD1
신종류%면역조직화학%Western blot%RT-PCR%ZNRD1
Renal neoplasms%Immunohistochemistry%Western blot%RT-PCR%ZNRD1
目的 研究转录相关锌带蛋白基因(ZNRD1)在肾癌及肾脏正常组织巾的表达.方法 应用免疫组化法,检测71例肾癌组织和24例癌旁正常肾脏组织中ZNRD1蛋白的表达,并分析ZNRD1蛋白表达与肾癌Gleason分级和临床分期的关系.应用逆转录聚合酶链反应(RT-PCR)和Western blot法,分别检测20例肾癌及痛旁正常肾脏组织中ZNRD1 mRNA和蛋白的表达.结果 免疫组化染色结果 显示,ZNRD1蛋白主要定位于细胞核.ZNRD1蛋白在24例肾癌及其相应的癌旁正常肾脏组织中的阳性表达率分别为91.7%(22/24)和20.8%(5/24),差异具有明显的统计学意义(P＜0.01).ZNRD1蛋白的表达与肾癌Gleason分级和临床分期均无显著的相关性(均P＞0.05).RT-PCR检测结果 显示,ZNRD1 mRNA在肾癌组织中的相对表达量为0.6186,明显高于癌旁正常肾脏组织(0.4273,P＜0.01).Western blot检测结果 湿示,ZRND1蛋白在肾癌组织中的相对表达量为0.5623,亦明显高于癌旁正常肾脏组织(0.3885,P＜0.01).结论 ZNRD1可能在肾癌的发生发展中发挥着重要作用.
目的 研究轉錄相關鋅帶蛋白基因(ZNRD1)在腎癌及腎髒正常組織巾的錶達.方法 應用免疫組化法,檢測71例腎癌組織和24例癌徬正常腎髒組織中ZNRD1蛋白的錶達,併分析ZNRD1蛋白錶達與腎癌Gleason分級和臨床分期的關繫.應用逆轉錄聚閤酶鏈反應(RT-PCR)和Western blot法,分彆檢測20例腎癌及痛徬正常腎髒組織中ZNRD1 mRNA和蛋白的錶達.結果 免疫組化染色結果 顯示,ZNRD1蛋白主要定位于細胞覈.ZNRD1蛋白在24例腎癌及其相應的癌徬正常腎髒組織中的暘性錶達率分彆為91.7%(22/24)和20.8%(5/24),差異具有明顯的統計學意義(P＜0.01).ZNRD1蛋白的錶達與腎癌Gleason分級和臨床分期均無顯著的相關性(均P＞0.05).RT-PCR檢測結果 顯示,ZNRD1 mRNA在腎癌組織中的相對錶達量為0.6186,明顯高于癌徬正常腎髒組織(0.4273,P＜0.01).Western blot檢測結果 濕示,ZRND1蛋白在腎癌組織中的相對錶達量為0.5623,亦明顯高于癌徬正常腎髒組織(0.3885,P＜0.01).結論 ZNRD1可能在腎癌的髮生髮展中髮揮著重要作用.
목적 연구전록상관자대단백기인(ZNRD1)재신암급신장정상조직건적표체.방법 응용면역조화법,검측71례신암조직화24례암방정상신장조직중ZNRD1단백적표체,병분석ZNRD1단백표체여신암Gleason분급화림상분기적관계.응용역전록취합매련반응(RT-PCR)화Western blot법,분별검측20례신암급통방정상신장조직중ZNRD1 mRNA화단백적표체.결과 면역조화염색결과 현시,ZNRD1단백주요정위우세포핵.ZNRD1단백재24례신암급기상응적암방정상신장조직중적양성표체솔분별위91.7%(22/24)화20.8%(5/24),차이구유명현적통계학의의(P＜0.01).ZNRD1단백적표체여신암Gleason분급화림상분기균무현저적상관성(균P＞0.05).RT-PCR검측결과 현시,ZNRD1 mRNA재신암조직중적상대표체량위0.6186,명현고우암방정상신장조직(0.4273,P＜0.01).Western blot검측결과 습시,ZRND1단백재신암조직중적상대표체량위0.5623,역명현고우암방정상신장조직(0.3885,P＜0.01).결론 ZNRD1가능재신암적발생발전중발휘착중요작용.
Objective To investigate the expression of zinc ribbon domain-containing1(ZNRD1)in human renal cell carcinonla and normal kidney tissues.Methods ThC expression of ZNRD1 protein was examined by immunohistochemical staining in 71 renal cell carcinomas and 24 samples of normal kidney tissue.The correlation between the expressions of ZNRD1 protein and clinicopathologic features was analyzed.The expression of ZNRD1 mRNA and ZNRD1 protein Was detected by quantitative reverse transeriptase-polymerase chain reaction(PT-PCR)and Western blot in 20 renal cell carcinomas and corresponding adjacent non-cancerous tissues.Results ZNRD1 protein Was detected mostly in the cell nuclei by immunohistochemistry.The positive expression rate of ZNRD1 protein was 91.7%(22/24)in renal cell carcinomas and 20.8%(5/24)in the normal kidney tissues,with a statistically significant difference between cancer and normal kidney tissue(P＜0.01).However,no significant correlation was observed between ZNRD1 protein expression level and clinicopathologic features(P＞0.05).ZNRD1 mRNA expression level was significantly higher in renal cell carcinomas(0.6186)than that in the normal kidney tissues(0.4273)assessed by RT-PCR(P＜0.01).The expression level of ZNRDl protein by Western blot was 0.5623 in renal cell carcinomas.significantly higher than that in normal kidney tissues (0.3885,P＜0.01).Conclusion ZNRD1 gene and ZNRD1 protein may play an important role in the carcinogenesis of renal cell carcinoma.Further investigation is still needed.
